FastQCFastQC Report
Fri 17 Jun 2016
SRR1526439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65780
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1940.2949224688355123No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.2782000608087564No Hit
TGTCACGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1790.2721191851626634No Hit
TGTCACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1770.2690787473396169No Hit
TGTCACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1610.24475524475524477No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1540.23411371237458192No Hit
TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.2325934934630587No Hit
TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.21587108543630282No Hit
TGTCACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1300.1976284584980237No Hit
TGTCACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1300.1976284584980237No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1290.19610823958650048No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1260.1915475828519307No Hit
TGTCACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1230.1869869261173609No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1140.17330495591365158No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1130.17178473700212832No Hit
TGTCACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1100.16722408026755853No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1070.16266342353298877No Hit
TGTCACGGGCAGGAGCAGTATGCGGTGTATTTGCATGCTCTCGTGAGAAATCTTTTTAATGAAG1040.15810276679841898No Hit
TGTCACGGGGACCGTGTTTGTGGGCAATTTAGAGGCCCGAGTGCGGGAAGAGATTCTTTACGAG1030.1565825478868957No Hit
TGTCACGGGGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGTAGAGATAT980.14898145332927942No Hit
TGTCACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG910.1383399209486166No Hit
TGTCACGGGACCAGATGGCAAGAAGTCTGCACCCTATCTCTCATTATGAAACCAAAACTGTCTC880.13377926421404682No Hit
TGTCACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG860.13073882639100032No Hit
TGTCACGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC850.12921860747947705No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG850.12921860747947705No Hit
TGTCACGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGACCGCGCCGG840.1276983885679538No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGC830.12617816965643053No Hit
TGTCACGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAATA770.11705685618729096No Hit
TGTCACGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC760.11553663727576771No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC730.11097598054119794No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG720.10945576162967469No Hit
TGTCACGGGTGATAATAAAAATTCCAGCTCCTCAGGTTTAACCTCTAAGTTGTGGATGGAGACT700.10641532380662815No Hit
TGTCACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC700.10641532380662815No Hit
TGTCACGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC700.10641532380662815No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC690.1048951048951049No Hit
TGTCACGGGCAGATAGTAATGGATATCCTGCAAGTCGGCTTCGTAGAGTCTCCCTTGAAAGTGG690.1048951048951049No Hit
TGTCACGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTT690.1048951048951049No Hit
TGTCACGGGGAGAGGGAAGGAGGCAAGATGGTGTTGGAGAGCACTATGGTTTGTGTGGACAACA680.10337488598358163No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC670.10185466707205838No Hit
TGTCACGGGAGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCT660.1003344481605351No Hit
TGTCACGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG660.1003344481605351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACG258.377723E-658.23236556
TTTACGG150.00459416858.2323651
TACGGCA150.00459416858.2323653
CCATCTA150.00459416858.2323656
TTACGGC150.00459416858.2323652
CCCCGTC258.415946E-658.1877450
AAAACTT150.004608148258.18773746
ATGAAAC150.004608148258.18773746
CAATGAG150.004608148258.18773748
TCATTAT150.004622159558.14318541
CATTATG150.004622159558.14318542
CTTGCCC150.004622159558.14318541
TGCGAAA150.004622159558.14318544
CTCATTA150.004622159558.14318540
ATCTGTT150.004622159558.14318545
AGTAAAA150.004622159558.14318543
TATGCGT201.9796024E-458.098732
ATGCGTT201.9796024E-458.098733
CCCTATC150.004636202558.09869432
GCGGTAC150.004636202558.09869430
Produced by FastQC (version 0.11.5)