FastQCFastQC Report
Fri 17 Jun 2016
SRR1526438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63791
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.2398457462651471No Hit
TGTCACGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1520.2382781270085122No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1510.2367105077518772No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1400.21946669592889279No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1300.2037905033625433No Hit
TGTCACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1280.2006552648492734No Hit
TGTCACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1270.19908764559263847No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1230.19281716856609868No Hit
TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.18654669153955886No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1140.17870859525638413No Hit
TGTCACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1140.17870859525638413No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1100.17243811822984434No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1030.1614647834333997No Hit
TGTCACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1030.1614647834333997No Hit
TGTCACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1020.15989716417676475No Hit
TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.15676192566349484No Hit
TGTCACGGGGACCGTGTTTGTGGGCAATTTAGAGGCCCGAGTGCGGGAAGAGATTCTTTACGAG850.13324763681397062No Hit
TGTCACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT840.1316800175573357No Hit
TGTCACGGGCAGGAGCAGTATGCGGTGTATTTGCATGCTCTCGTGAGAAATCTTTTTAATGAAG840.1316800175573357No Hit
TGTCACGGGGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGTAGAGATAT770.12070668276089104No Hit
TGTCACGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG720.1128685864777163No Hit
TGTCACGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTT710.11130096722108133No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG700.10973334796444639No Hit
TGTCACGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC670.10503049019454154No Hit
TGTCACGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG640.1003276324246367No Hit
TGTCACGGGACCAGATGGCAAGAAGTCTGCACCCTATCTCTCATTATGAAACCAAAACTGTCTC640.1003276324246367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAG150.00451941158.47180633
CGCTGAT150.00451941158.47180634
TTACAGT150.00451941158.47180634
AGCATGC150.00454793558.37906635
TGATTCC150.00454793558.37906637
TTGCGAT150.00454793558.37906635
TACAGTA150.00454793558.37906635
ATCGATG150.00454793558.37906635
CGATGAA201.9325262E-458.37906337
TTGCATC258.250565E-658.37906337
CAACACT150.00457659358.2866238
ATTCCCC150.00457659358.2866239
AACACTC150.00457659358.2866239
TGCTCAT201.9477487E-458.28661739
CGTGCTT258.44675E-658.148532
CGACCTG150.004619832658.148554
CCTACCG258.44675E-658.148557
TGAAACA150.004619832658.148558
CGAGGAT150.004619832658.148531
CTACCGC258.44675E-658.148558
Produced by FastQC (version 0.11.5)