##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526438_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63791 Sequences flagged as poor quality 0 Sequence length 64 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.250928814409555 39.0 38.0 40.0 35.0 40.0 2 37.79542568700914 39.0 38.0 40.0 35.0 40.0 3 37.986440093430105 39.0 38.0 40.0 35.0 40.0 4 38.068034675737955 39.0 38.0 40.0 35.0 40.0 5 38.20416673198414 39.0 38.0 40.0 35.0 40.0 6 38.14149331410387 39.0 38.0 40.0 35.0 40.0 7 38.27790754181624 39.0 38.0 40.0 36.0 40.0 8 38.16836230816259 39.0 38.0 40.0 36.0 40.0 9 38.36270006740763 39.0 38.0 40.0 36.0 40.0 10 37.625433054819645 39.0 38.0 40.0 33.0 40.0 11 37.52689250834757 39.0 38.0 40.0 33.0 40.0 12 37.26486494960104 39.0 37.0 40.0 33.0 40.0 13 37.21609631452713 39.0 37.0 40.0 32.0 40.0 14 37.194729664059196 39.0 37.0 40.0 33.0 40.0 15 37.005439638820526 39.0 36.0 40.0 33.0 40.0 16 36.7182047624273 39.0 36.0 40.0 31.0 40.0 17 36.388456051794144 38.0 36.0 40.0 31.0 40.0 18 36.62458654042106 38.0 36.0 40.0 31.0 40.0 19 36.60373720430782 38.0 36.0 40.0 31.0 40.0 20 36.464783433399695 38.0 35.0 39.0 31.0 40.0 21 36.41900895110596 38.0 36.0 39.0 31.0 40.0 22 36.24558323274443 38.0 35.0 39.0 30.0 40.0 23 36.0774717436629 38.0 35.0 39.0 30.0 40.0 24 35.89225752849148 38.0 35.0 39.0 29.0 40.0 25 35.85623363797401 38.0 35.0 39.0 29.0 40.0 26 36.00893542976282 38.0 35.0 40.0 30.0 40.0 27 35.49322004671505 38.0 35.0 40.0 28.0 40.0 28 35.39649793858068 38.0 35.0 39.0 28.0 40.0 29 35.2884576194134 38.0 35.0 39.0 28.0 40.0 30 35.26107131099999 38.0 35.0 39.0 28.0 40.0 31 34.882914517721936 38.0 35.0 39.0 26.0 40.0 32 34.90931322600367 38.0 34.0 39.0 27.0 40.0 33 34.87278769732407 38.0 34.0 39.0 27.0 40.0 34 34.26044426329733 38.0 33.0 39.0 24.0 40.0 35 34.32421501465724 38.0 33.0 39.0 25.0 40.0 36 33.62264269254283 38.0 33.0 39.0 21.0 40.0 37 33.394099481118026 38.0 33.0 39.0 21.0 40.0 38 33.2734555031274 37.0 32.0 39.0 21.0 40.0 39 32.80022260193444 37.0 32.0 39.0 17.0 40.0 40 32.841231521688016 37.0 32.0 39.0 18.0 40.0 41 32.2735025317051 36.0 31.0 39.0 12.0 40.0 42 32.06220313210328 36.0 31.0 39.0 10.0 40.0 43 31.89591008135944 36.0 30.0 39.0 10.0 40.0 44 31.700412283864495 36.0 30.0 39.0 8.0 40.0 45 31.48000501638162 36.0 30.0 39.0 4.0 40.0 46 31.142966876205108 36.0 30.0 39.0 4.0 40.0 47 30.884325375052907 35.0 29.0 39.0 4.0 40.0 48 30.60557131883808 35.0 29.0 39.0 4.0 40.0 49 30.436958191594425 35.0 29.0 39.0 4.0 40.0 50 29.994858208838238 35.0 28.0 39.0 4.0 40.0 51 29.574250286090514 35.0 28.0 38.0 4.0 40.0 52 29.411829254910568 35.0 27.0 38.0 4.0 40.0 53 29.06411562759637 34.0 26.0 38.0 4.0 40.0 54 28.801586430687713 34.0 26.0 38.0 4.0 40.0 55 28.371823611481243 34.0 24.0 38.0 4.0 40.0 56 27.774388236585096 34.0 21.0 38.0 4.0 40.0 57 27.42151714191657 33.0 20.0 38.0 4.0 39.0 58 27.067266542302207 33.0 18.0 38.0 4.0 39.0 59 26.784969666567385 33.0 17.0 37.0 4.0 39.0 60 26.463968271386246 33.0 12.0 37.0 4.0 39.0 61 26.209543666034392 33.0 4.0 37.0 4.0 39.0 62 25.849163675126587 33.0 4.0 37.0 4.0 39.0 63 25.62562116913044 33.0 4.0 37.0 4.0 39.0 64 25.503315514727785 33.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.5469231935645169 2 2 -0.6003970373560463 2 3 -0.8642244206261367 2 4 -0.08509206978848738 2 5 -0.028684593337104047 2 6 0.16590032477746064 2 7 -0.2589565460485872 2 8 0.21109196376824002 2 9 -0.00589993337423067 2 10 -0.1082189427184801 2 11 -0.5241803682995965 2 12 -0.32458867199545693 2 13 0.08890523370415337 2 14 -0.2806233157601241 2 15 -0.4530571087571289 2 16 -0.49878024035276525 2 17 -0.13197500933404172 2 18 0.1021775903414266 2 19 -0.06177378451896942 2 20 -0.5487108457646386 2 21 -0.13957772244708622 2 22 -0.2717061993345027 2 23 0.17759596540536648 2 24 -1.1168054600801796 2 25 -0.22277610777855017 2 26 -0.09956737552013095 2 27 0.20550146989764073 2 28 -0.08192932011012033 2 29 0.2099802881248749 2 30 -0.313839224228289 2 31 0.30322374426729226 2 32 -0.21461859024199725 2 33 0.033753484204638085 2 34 0.3245708813456005 2 35 -0.08804188183820116 2 36 0.671120092810213 2 37 0.22474885613775086 2 38 -0.4662797889989321 2 39 -2.7727331504079693 2 40 -0.9471542262430823 2 41 -1.0158161538557309 2 42 -1.2417081602950972 2 43 -1.1843344850182334 2 44 -1.2489037075545113 2 45 -1.374414538537561 2 46 -0.49586449364944585 2 47 -0.7571102232649167 2 48 -1.1771859039023447 2 49 0.09619174434770983 2 50 -0.24858803059280632 2 51 -0.985025890634514 2 52 -1.5711310824029248 2 53 -0.7443485194614965 2 54 -0.9377877846141693 2 55 -0.34960432170975864 2 56 -1.8585124982851724 2 57 -0.7910720821751838 2 58 -1.153029848841662 2 59 -0.7371086974760885 2 60 -0.9369950008604633 2 61 -1.726539220260591 2 62 -2.2587753741575085 2 63 -1.7688722743728853 2 64 -2.258920191878282 3 1 -0.057064899593967766 3 2 0.16259268201044108 3 3 -0.5980393692229669 3 4 0.04411048647714466 3 5 0.10301865756592576 3 6 0.06809537895917828 3 7 -0.09113215038312461 3 8 -0.17151431575385345 3 9 -0.09509137544146995 3 10 -0.067929973560382 3 11 0.26381629744088997 3 12 -0.828895423870982 3 13 -0.5582189674628353 3 14 -0.7521431823897444 3 15 -0.3866497733861962 3 16 0.4606529355294242 3 17 -0.43650404517732966 3 18 -0.16928670529625123 3 19 -0.11123196734752838 3 20 0.4579576732684316 3 21 0.6903750422097588 3 22 1.1370184464004254 3 23 0.36598162808945034 3 24 -0.616110822680902 3 25 0.6577462595457106 3 26 -0.06344623075769817 3 27 0.6406223368051158 3 28 0.7774760702761014 3 29 0.751241749641963 3 30 0.6058606924152272 3 31 0.36490754532314185 3 32 0.4714053052845344 3 33 0.8086909668387037 3 34 -0.10360361156909903 3 35 -1.7340546631663543 3 36 -4.842633727695485 3 37 -4.4657207187441585 3 38 -4.916960533650226 3 39 -19.005575606645813 3 40 -6.726259533272813 3 41 -5.545407707064957 3 42 -5.642652867158116 3 43 -6.319094140478082 3 44 -5.110254082658706 3 45 -5.194642379604524 3 46 -2.125761687314352 3 47 -1.4664739354071799 3 48 -1.3227819027909256 3 49 -2.182774635202165 3 50 -1.3249981445133372 3 51 -1.841097021504197 3 52 -0.9770771785407426 3 53 -1.510672498344519 3 54 -1.4776599713327023 3 55 -1.6169007929517711 3 56 -1.8924108033699198 3 57 -1.3898495203524526 3 58 -2.2508347946599443 3 59 -2.5759528208436926 3 60 -2.314322036148045 3 61 -2.805450028818523 3 62 -2.669445004610413 3 63 -3.0408922799299845 3 64 -1.8343578134398264 4 1 -0.12348660677316303 4 2 0.5328583688391575 4 3 -0.07316655972608288 4 4 0.4935169307959697 4 5 -0.04621819941542071 4 6 0.5449184428370728 4 7 -0.0023814437692166734 4 8 0.3174625073100259 4 9 -0.0021009853905908926 4 10 0.5861118579828641 4 11 -0.0592475804562298 4 12 -0.43008253523333195 4 13 0.31713433949024505 4 14 0.2000897175537233 4 15 -0.5828057937366751 4 16 0.4535867964678104 4 17 -0.16148991289920644 4 18 0.1673441595991676 4 19 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0.1506415733922708 119 19 -0.36712336079015273 119 20 0.639053984743839 119 21 0.4239815995868099 119 22 -0.18060450441925013 119 23 -0.5453913227302252 119 24 -0.6268690194022142 119 25 0.38315609561128383 119 26 0.1740025397341043 119 27 0.8985629105756061 119 28 0.27056008666954057 119 29 0.7184548643960582 119 30 0.34638323339883215 119 31 -0.2685453235293167 119 32 -0.22838977668267546 119 33 -0.7681532954563792 119 34 0.45274884744729604 119 35 -0.10393171234667875 119 36 0.11100356738648287 119 37 0.7123018222394464 119 38 -0.3526675008633404 119 39 0.4536969343377919 119 40 0.43075890935013916 119 41 0.7691414732629127 119 42 1.2326032803828681 119 43 0.36174602345634455 119 44 0.7455348517675233 119 45 0.5773863089200653 119 46 -0.020228900429106744 119 47 -0.017703443603899416 119 48 -0.2584888700040402 119 49 -0.631032831923477 119 50 -0.4384715051690762 119 51 -0.6889453821599361 119 52 -0.4681120146063158 119 53 -0.6172298753937007 119 54 -0.10701447952942189 119 55 -0.15839669459111505 119 56 -0.14241080336991985 119 57 0.8212160534180377 119 58 1.0045443037007118 119 59 1.4906455398120464 119 60 1.3357804228683499 119 61 1.929181118722461 119 62 2.184550897028931 119 63 1.5907675561355887 119 64 2.0808573504946004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 45.0 5 6.0 6 13.0 7 18.0 8 58.0 9 49.0 10 103.0 11 101.0 12 149.0 13 196.0 14 254.0 15 303.0 16 403.0 17 435.0 18 471.0 19 522.0 20 625.0 21 741.0 22 951.0 23 920.0 24 1050.0 25 994.0 26 1041.0 27 1253.0 28 1411.0 29 1587.0 30 1947.0 31 2253.0 32 2693.0 33 3396.0 34 4272.0 35 5405.0 36 6780.0 37 8350.0 38 9132.0 39 5847.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.36041134329294 0.4593124421940399 0.09562477465473186 0.08465143985828721 8 99.36313725490196 0.33098039215686276 0.2117647058823529 0.09411764705882353 9 98.86190841968303 0.5737486479283911 0.1943847878227336 0.36995814456584786 10 60.80481572635639 25.318618613911053 4.760859682400339 9.115705977332226 11 35.93453621984292 27.596369393801634 15.251367747801414 21.217726638554026 12 32.58923672618395 16.398865043658194 25.981721559467637 25.030176670690224 13 24.82952140584095 17.171701337179226 29.58254299195811 28.41623426502171 14 25.638412942264583 19.712812152184476 31.107836528663917 23.54093837688702 15 27.438890457811667 18.359534343719602 28.896419718221466 25.30515548024726 16 28.84561106407 20.409571298648352 24.05525762850064 26.68956000878101 17 30.906722741824115 20.46324775594752 22.247504864729144 26.382524637499216 18 29.18907055854274 20.8665799250678 22.42479346616294 27.519556050226523 19 30.402407863178194 19.8977912244674 21.77579909391607 27.924001818438338 20 28.410605371674063 22.149923956161118 22.2753570924599 27.164113579704917 21 30.234672602718256 18.831810129955638 24.5536204166732 26.379896850652912 22 29.41637535075481 20.189368406201503 25.16028906899092 25.23396717405277 23 31.782411036212572 20.97037153158802 23.088258347703402 24.158959084496 24 28.17100126981141 23.234413456865603 23.240684130492717 25.35390114283027 25 29.100919158013618 21.179847538977945 23.463625811713776 26.255607491294665 26 28.295467017890463 19.936654279756024 24.174859275287332 27.593019427066185 27 26.433261081267865 18.08186473156787 25.040052775421735 30.444821411742527 28 27.221629089995602 20.10456572253972 25.178986371914842 27.49481881554983 29 27.322533044488402 20.680669366738876 25.702489717748268 26.294307871024458 30 26.26503753494362 18.73920281433552 28.056977730313786 26.938781920407074 31 27.293577981651374 19.94156088978258 27.973796657031546 24.7910644715345 32 28.784048983436687 18.500667242326713 29.54549022686239 23.169793547374205 33 29.470344307614976 18.569364161849713 29.013257099773814 22.947034430761498 34 26.172870583052767 20.329756772914635 32.20634970579906 21.291022938233535 35 26.87019782222711 23.006457897962072 30.36311927470421 19.76022500510661 36 28.72777017783858 20.6377659323553 29.068981241253518 21.565482648552607 37 28.310107269810313 22.139733870206676 27.25628362012017 22.293875239862846 38 28.07290683776813 21.832735736302446 29.15487198842549 20.939485437503933 39 27.229691346335922 21.24597614088241 28.832923057501738 22.691409455279935 40 26.19856549641374 20.5187492135397 29.673147099534415 23.609538190512144 41 23.122433244693404 21.51275313517851 31.134643525876037 24.230170094252042 42 24.669207989414655 18.064394178060613 31.47564740722072 25.790750425304015 43 24.377106498251802 18.41433836267994 32.89129681544713 24.31725832362113 44 24.263270574900826 17.80272023172344 33.65027391222215 24.28373528115358 45 23.75366107139483 17.699115044247787 32.52606052971373 26.021163354643654 46 24.56927777252827 19.438407508897917 30.845065986330276 25.147248732243536 47 24.829519504858496 19.58202749736208 32.902341842921714 22.68611115485771 48 26.805260256713126 20.377982518308528 31.819828332939604 20.996928892038742 49 26.576675907258064 19.896673387096776 30.69241431451613 22.834236391129032 50 21.938067608476285 21.487134207870838 31.186932391523715 25.387865792129162 51 23.639801465374617 21.824627747577406 31.36689513905302 23.168675647994956 52 24.721438590408347 21.378701675308506 29.804100802193815 24.09575893208933 53 23.937645797868985 21.12098858836139 31.418258621776683 23.52310699199294 54 25.821041351487644 20.14151538073626 29.982980332829044 24.05446293494705 55 25.75432733234543 20.56625784279724 29.340732099504997 24.338682725352335 56 23.776411226742354 21.865342163355407 28.79060233364869 25.56764427625355 57 24.176586519511233 22.207331493890422 28.3878596767836 25.22822230981474 58 24.451702483511628 23.238347691628007 28.342642557354285 23.967307267506076 59 26.063149921891025 23.18968645953324 27.944077130639233 22.803086487936504 60 25.72853850282575 22.740188804344395 26.588892747766234 24.94237994506362 61 24.984626058436746 22.728204481306864 26.838959933143062 25.448209527113324 62 22.82469836763662 22.100780695528744 28.207554609257947 26.86696632757669 63 24.040251096179933 22.289517680830258 26.82565218762815 26.84457903536166 64 24.64115965829241 22.616810623884792 25.20646149473385 27.535568223088948 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 2.0 7 1.5 8 1.0 9 7.5 10 39.0 11 64.0 12 55.5 13 70.0 14 93.0 15 157.5 16 347.5 17 473.0 18 481.0 19 489.0 20 526.5 21 682.0 22 800.0 23 877.5 24 1133.0 25 1311.0 26 1311.5 27 1200.0 28 1088.0 29 988.0 30 854.5 31 821.0 32 711.0 33 601.0 34 582.0 35 586.5 36 610.0 37 563.0 38 602.0 39 688.0 40 691.5 41 797.5 42 900.0 43 799.0 44 698.0 45 830.0 46 1020.0 47 1078.0 48 1085.5 49 1286.5 50 1480.0 51 1521.5 52 1724.5 53 1886.0 54 2346.0 55 3080.5 56 3355.0 57 3330.0 58 3305.0 59 3929.0 60 4726.0 61 4899.0 62 4850.5 63 4397.5 64 3993.0 65 3829.5 66 3166.5 67 2667.0 68 2305.0 69 1943.0 70 1772.5 71 1417.0 72 1232.0 73 1131.0 74 808.0 75 586.0 76 468.5 77 285.5 78 220.0 79 178.0 80 100.5 81 65.0 82 56.0 83 47.0 84 40.5 85 24.0 86 14.0 87 8.0 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.06427238952203289 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08308382060165227 16 0.026649527362794127 17 0.10503049019454154 18 0.0 19 0.0 20 0.01881143107961938 21 0.0 22 0.0 23 0.0015676192566349484 24 0.003135238513269897 25 0.058001912495493094 26 0.02194666959288928 27 0.1959524070793686 28 0.1551943064068599 29 0.1395181138405104 30 0.18341145302628897 31 0.21162859964571806 32 0.15049144863695504 33 0.19908764559263847 34 0.3589848097694032 35 0.23200764998197237 36 0.30411813578718 37 0.33390290166324404 38 0.31822670909689454 39 0.6568324685300434 40 0.33703814017651396 41 0.3730933830791177 42 0.4812591117869292 43 0.46558291922057965 44 0.41855434152153126 45 0.44677148814096035 46 0.4593124421940399 47 0.4608800614506749 48 0.46401529996394475 49 0.4749886347603894 50 0.5753162671850262 51 0.5110438776629932 52 0.5329905472558825 53 0.5439638820523272 54 0.5251524509727078 55 0.5596400746186767 56 0.5815867442115659 57 0.5737486479283911 58 0.6474267529902337 59 0.6552648492734084 60 0.6975905692025521 61 0.5831543634682008 62 0.6051010330610901 63 0.609803890830995 64 0.7242400965653462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 63791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46051950902165 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18071723895258 42.330422786913516 2 8.318916224379088 8.894671662146699 3 3.803184470574436 6.099606527566585 4 2.3868867841538868 5.104168299603392 5 1.598099873911386 4.2717624743302345 6 1.0820162449051403 3.470709034189776 7 0.7360056300032255 2.7543070339076046 8 0.5307450957393778 2.2699126836074055 9 0.38999501510131074 1.8764402501920334 >10 1.841480221681377 17.011804173002464 >50 0.08796880039879189 2.986314683889577 >100 0.043984400199395944 2.9298803906507187 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.2398457462651471 No Hit TGTCACGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 152 0.2382781270085122 No Hit TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 151 0.2367105077518772 No Hit TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG 140 0.21946669592889279 No Hit TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 130 0.2037905033625433 No Hit TGTCACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT 128 0.2006552648492734 No Hit TGTCACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC 127 0.19908764559263847 No Hit TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 123 0.19281716856609868 No Hit TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 0.18654669153955886 No Hit TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC 114 0.17870859525638413 No Hit TGTCACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC 114 0.17870859525638413 No Hit TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 110 0.17243811822984434 No Hit TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 103 0.1614647834333997 No Hit TGTCACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA 103 0.1614647834333997 No Hit TGTCACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 102 0.15989716417676475 No Hit TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.15676192566349484 No Hit TGTCACGGGGACCGTGTTTGTGGGCAATTTAGAGGCCCGAGTGCGGGAAGAGATTCTTTACGAG 85 0.13324763681397062 No Hit TGTCACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT 84 0.1316800175573357 No Hit TGTCACGGGCAGGAGCAGTATGCGGTGTATTTGCATGCTCTCGTGAGAAATCTTTTTAATGAAG 84 0.1316800175573357 No Hit TGTCACGGGGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGTAGAGATAT 77 0.12070668276089104 No Hit TGTCACGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG 72 0.1128685864777163 No Hit TGTCACGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTT 71 0.11130096722108133 No Hit TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG 70 0.10973334796444639 No Hit TGTCACGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC 67 0.10503049019454154 No Hit TGTCACGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG 64 0.1003276324246367 No Hit TGTCACGGGACCAGATGGCAAGAAGTCTGCACCCTATCTCTCATTATGAAACCAAAACTGTCTC 64 0.1003276324246367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0015676192566349484 0.0 0.0 0.0 0.0 9 0.0015676192566349484 0.0 0.0 0.0 0.0 10 0.003135238513269897 0.0 0.0 0.0 0.0 11 0.007838096283174743 0.0 0.0 0.0 0.0 12 0.007838096283174743 0.0 0.0 0.0 0.0 13 0.00940571553980969 0.0 0.0 0.0 0.0 14 0.012540954053079588 0.0 0.0 0.0 0.0 15 0.014108573309714536 0.0 0.0 0.0 0.0 16 0.01881143107961938 0.0 0.0 0.0 0.0 17 0.023514288849524227 0.0 0.0 0.0 0.0 18 0.028217146619429073 0.0 0.0 0.0 0.0 19 0.02978476587606402 0.0 0.0 0.0 0.0 20 0.03135238513269897 0.0 0.0 0.0 0.0 21 0.03919048141587371 0.0 0.0 0.0 0.0 22 0.05016381621231835 0.0 0.0 0.0 0.0 23 0.0517314354689533 0.0 0.0 0.0 0.0 24 0.058001912495493094 0.0 0.0 0.0 0.0 25 0.06270477026539795 0.0 0.0 0.0 0.0 26 0.06427238952203289 0.0 0.0 0.0 0.0 27 0.06740762803530279 0.0 0.0 0.0 0.0 28 0.07367810506184258 0.0 0.0 0.0 0.0 29 0.07681334357511248 0.0 0.0 0.0 0.0 30 0.08621905911492217 0.0 0.0 0.0 0.0 31 0.08935429762819207 0.0 0.0 0.0 0.0 32 0.09248953614146196 0.0 0.0 0.0 0.0 33 0.10189525168127166 0.0 0.0 0.0 0.0 34 0.1065981094511765 0.0 0.0 0.0 0.0 35 0.11757144424762114 0.0 0.0 0.0 0.0 36 0.11913906350425608 0.0 0.0 0.0 0.0 37 0.12227430201752598 0.0 0.0 0.0 0.0 38 0.1254095405307959 0.0 0.0 0.0 0.0 39 0.12697715978743082 0.0 0.0 0.0 0.0 40 0.13011239830070073 0.0 0.0 0.0 0.0 41 0.13481525607060557 0.0 0.0 0.0 0.0 42 0.1395181138405104 0.0 0.0 0.0 0.0 43 0.14735621012368516 0.0 0.0 0.0 0.0 44 0.15049144863695504 0.0 0.0 0.0 0.0 45 0.1551943064068599 0.0 0.0 0.0 0.0 46 0.1614647834333997 0.0 0.0 0.0 0.0 47 0.1646000219466696 0.0 0.0 0.0 0.0 48 0.16616764120330454 0.0 0.0 0.0 0.0 49 0.17087049897320938 0.0 0.0 0.0 0.0 50 0.17870859525638413 0.0 0.0 0.0 0.0 51 0.18184383376965402 0.0 0.0 0.0 0.0 52 0.18968193005282877 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACAG 15 0.004519411 58.471806 33 CGCTGAT 15 0.004519411 58.471806 34 TTACAGT 15 0.004519411 58.471806 34 AGCATGC 15 0.004547935 58.379066 35 TGATTCC 15 0.004547935 58.379066 37 TTGCGAT 15 0.004547935 58.379066 35 TACAGTA 15 0.004547935 58.379066 35 ATCGATG 15 0.004547935 58.379066 35 CGATGAA 20 1.9325262E-4 58.379063 37 TTGCATC 25 8.250565E-6 58.379063 37 CAACACT 15 0.004576593 58.28662 38 ATTCCCC 15 0.004576593 58.28662 39 AACACTC 15 0.004576593 58.28662 39 TGCTCAT 20 1.9477487E-4 58.286617 39 CGTGCTT 25 8.44675E-6 58.1485 32 CGACCTG 15 0.0046198326 58.1485 54 CCTACCG 25 8.44675E-6 58.1485 57 TGAAACA 15 0.0046198326 58.1485 58 CGAGGAT 15 0.0046198326 58.1485 31 CTACCGC 25 8.44675E-6 58.1485 58 >>END_MODULE