FastQCFastQC Report
Fri 17 Jun 2016
SRR1526437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60846
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2160.35499457647174837No Hit
TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.30075929395523127No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1720.28268086644972557No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1520.24981099825789696No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1500.24652401143871414No Hit
TGTCACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1480.24323702461953128No Hit
TGTCACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC1450.23830654439075696No Hit
TGTCACGGGCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT1330.21858462347565988No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1300.21365414324688559No Hit
TGTCACGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT1230.2021496893797456No Hit
TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.19228872892219703No Hit
TGTCACGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC1140.18735824869342274No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1110.18242776846464845No Hit
TGTCACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1100.18078427505505704No Hit
TGTCACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1080.17749728823587418No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1040.17092331459750845No Hit
TGTCACGGGCAGGAGCAGTATGCGGTGTATTTGCATGCTCTCGTGAGAAATCTTTT950.15613187391118563No Hit
TGTCACGGGGACCGTGTTTGTGGGCAATTTAGAGGCCCGAGTGCGGGAAGAGATTC930.15284488709200278No Hit
TGTCACGGGACCAGATGGCAAGAAGTCTGCACCCTATCTCTCATTATGAAACCAAA910.1495579002728199No Hit
TGTCACGGGAGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGC910.1495579002728199No Hit
TGTCACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA900.14791440686322846No Hit
TGTCACGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGC870.1429839266344542No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC850.13969693981527134No Hit
TGTCACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA790.12983597935772279No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA790.12983597935772279No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG790.12983597935772279No Hit
TGTCACGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAA750.12326200571935707No Hit
TGTCACGGGGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGT740.12161851230976564No Hit
TGTCACGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTG740.12161851230976564No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGG710.11668803208099135No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA700.11504453867139994No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC700.11504453867139994No Hit
TGTCACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT680.11175755185221707No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG670.11011405844262566No Hit
TGTCACGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCC650.10682707162344279No Hit
TGTCACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC630.10354008480425994No Hit
TGTCACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC630.10354008480425994No Hit
TGTCACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.1018965913946685No Hit
TGTCACGGGCTTCTACAAACAAATTATTTTTATTTGTTTTAAAATTGTAAAATACT610.10025309798507707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGC150.008044640550.53801337
AAAGGAT150.008044640550.53801336
ATCGACA150.008044640550.53801335
CGGTATT150.008044640550.53801336
GGTCTGA150.00807127250.4958334
CGACTCA203.9873927E-450.41166732
AATCTTG150.00815155650.36969431
CGGTCAA150.00815155650.36969431
CCGACTC204.0038422E-450.36969431
GTTTGCA150.00823243550.2441946
ACTCGAA150.00823243550.2441946
GAAATCT204.0535143E-450.2441947
TGAGAAA204.07018E-450.2024944
AATCTTT204.07018E-450.2024949
CTCCAGG204.07018E-450.2024943
CCCCGTC204.07018E-450.2024950
TGCAGAC204.07018E-450.2024949
GTAGAGT150.00825952650.20248843
TTACTCG150.00825952650.20248844
TTTCAAG150.00825952650.20248845
Produced by FastQC (version 0.11.5)