FastQCFastQC Report
Fri 17 Jun 2016
SRR1526436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141036
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.5743214498425934No Hit
CCATCTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4230.29992342380668763No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.26376244363141327No Hit
CCATCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.2609262883235486No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3680.2609262883235486No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3530.2502907059190561No Hit
CCATCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3360.23823704586063135No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2980.21129357043591707No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2970.2105845316089509No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2320.1644970078561502No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2290.1623698913752517No Hit
CCATCTGGGGTTTAAGCAAACAAAAACATCCCTGTCGCAGTCCCAGACACCAGAAGTCTAAAAG2170.15386142545165774No Hit
CCATCTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2130.15102527014379308No Hit
CCATCTGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA1920.13613545477750363No Hit
CCATCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1890.1340083382966051No Hit
CCATCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1840.1304631441617743No Hit
CCATCTGGGATGCTTCCTCGGGGAAATTTGACAGCATGTGGCCAGAGTTAGCCAGCACGCTTGA1810.12833602768087582No Hit
CCATCTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1720.12195467823818032No Hit
CCATCTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.11982756175728183No Hit
CCATCTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1690.11982756175728183No Hit
CCATCTGGGGTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC1660.11770044527638333No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1580.11202813466065402No Hit
CCATCTGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA1500.10635582404492469No Hit
CCATCTGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA1420.10068351342919539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC150.0046230358.17552658
GTCTAAA201.9780327E-458.1547856
TAGCCAG406.9485395E-1058.1547849
CGCTAAC201.9780327E-458.1547855
TCGCTAA201.9780327E-458.1547854
GTCATAG258.494886E-658.15477846
TTGGCCC150.00462956658.15477842
TTAATAT150.00462956658.15477857
ATAGGAC150.00462956658.15477845
CCCTAGG150.00462956658.15477849
CATTAAT150.00462956658.15477855
CCCGTCG150.00462956658.15477854
CCACTAT150.00462956658.15477853
TCTAATG150.00462956658.15477851
CCCCCGT150.00462956658.15477852
AGATTGT150.00462956658.15477857
TAGGGCA258.494886E-658.15477846
TCATAGC258.494886E-658.15477847
AATGTCG150.00462956658.15477854
CTAATGT150.00462956658.15477852
Produced by FastQC (version 0.11.5)