FastQCFastQC Report
Fri 17 Jun 2016
SRR1526435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136706
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.503269790645619No Hit
CCATCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.2560238760551841No Hit
CCATCTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3230.23627346275949845No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2980.21798604304127103No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.21725454625254195No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2860.20920808157652188No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2630.19238365543575264No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2580.18872617149210713No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2370.1733647389287961No Hit
CCATCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2220.16239228709785963No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1960.1433733705909031No Hit
CCATCTGGGGTTTAAGCAAACAAAAACATCCCTGTCGCAGTCCCAGACACCAGAAGTCTAAAAG1760.12874343481632117No Hit
CCATCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1750.12801193802759206No Hit
CCATCTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1680.12289146050648839No Hit
CCATCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1620.1185024797741138No Hit
CCATCTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1610.1177709829853847No Hit
CCATCTGGGATGCTTCCTCGGGGAAATTTGACAGCATGTGGCCAGAGTTAGCCAGCACGCTTGA1540.11265050546428101No Hit
CCATCTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.10533553757699005No Hit
CCATCTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1430.10460404078826094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA452.910383E-1158.44349737
TTATACG150.004552651758.40022339
CCACGCA150.004552651758.40022339
ACCGGCT150.00458612758.29231633
ATGTTCG150.00458612758.29231634
AATTGAG150.004599568458.24926457
TAACTTA258.4119965E-658.24926458
ACTGATA150.004606358.2277654
CAATTGA150.004606358.2277656
CTGACGT150.004606358.2277655
ACAATTG150.004606358.2277655
GCTAACT258.430441E-658.2277656
TACCGAA201.9655695E-458.22775754
ATTAAAG150.00461978558.18480353
TACTGAT150.00461978558.18480353
ATCGCTA303.6555866E-758.18480353
GGTAACA150.004626538658.1633541
TCTACCG201.9763602E-458.1633552
TATTCTC150.004626538658.1633542
AACAACC150.004646843858.09908344
Produced by FastQC (version 0.11.5)