FastQCFastQC Report
Fri 17 Jun 2016
SRR1526434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126110
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8900.7057330901593847No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.3544524621362303No Hit
CCATCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.3132186186662438No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3130.24819601934818808No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3100.24581714376338118No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3040.24105939259376732No Hit
CCATCTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3040.24105939259376732No Hit
CCATCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2920.2315438902545397No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2220.1760367932757117No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2080.16493537387994608No Hit
CCATCTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1930.1530409959559115No Hit
CCATCTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1830.1451114106732218No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1810.14352549361668385No Hit
CCATCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1780.14114661803187695No Hit
CCATCTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG1700.13480294980572516No Hit
CCATCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1630.12925224010784236No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1580.1252874474664975No Hit
CCATCTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.12370153040995954No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1540.12211561335342162No Hit
CCATCTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1530.12132265482515264No Hit
CCATCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1500.11894377924034573No Hit
CCATCTGGGGTTTAAGCAAACAAAAACATCCCTGTCGCAGTCCCAGACACCAGAAG1410.11180715248592499No Hit
CCATCTGGGATGCTTCCTCGGGGAAATTTGACAGCATGTGGCCAGAGTTAGCCAGC1410.11180715248592499No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1350.10704940131631116No Hit
CCATCTGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA1290.10229165014669732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGTA203.9233803E-450.6200137
TATCTGA150.00827213150.21488650
GCTATCT204.0902584E-450.194848
TCTTGTG150.00828522950.19479848
GAAGGTT150.00828522950.19479848
CACATGA150.00829834350.1747347
GAGTCTA204.106441E-450.1546839
TCGGATG204.106441E-450.1546846
CCTATTG550.050.1546840
ATGCATT204.106441E-450.1546846
CCATACC150.00831147350.15467539
AGACACG150.00831147350.15467539
TTACAAA150.00831147350.15467546
AGTTTAA204.1145514E-450.1346438
CGTGATT204.1145514E-450.1346438
AAAGTAG204.1145514E-450.1346438
TTGTCAT700.050.13463644
ATTGTCA700.050.13463643
TAGCTCG150.008324617550.13463644
TATTTTC204.1308103E-450.09461242
Produced by FastQC (version 0.11.5)