FastQCFastQC Report
Fri 17 Jun 2016
SRR1526433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13262
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGAAAATGCTAATATAT1491.1235107826873776No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGGGGGTTGCG570.42979942693409745No Hit
CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.4071784044638818No Hit
CGTGGCGGGACATTTAGGTCTATAATGCTTCTAAAACTGGTTTTGTATATGGCAGAGACTATTT540.4071784044638818No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.37701704117026086No Hit
CGTGGCGAGAGAAAAGGCATCGATATATTGATAATCTACTGTACCAAAAGTTGGCATCCGAGAA470.3543960187000453No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.27145226964258784No Hit
CGTGGCGGGATCTTTCATCTACCTATCTAAGGCATCTTCTTCCACTCTCATAATCCATCTGTAT360.27145226964258784No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.2563715879957774No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGGGGTTTGCG300.22621022470215654No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.18850852058513043No Hit
CGTGGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.18850852058513043No Hit
CGTGGCGGGGTGCAGTGCTCGTGGCGGAGATTAAGTAAGTTCTGCATATTTCTAAGTAGCCACA250.18850852058513043No Hit
CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.18096817976172522No Hit
CGTGGCGGGATGTGCCAAGTTCTGGGTTCCATCTCCGCCTGGCAAGACAAACACAGGAAACAAA220.1658874981149148No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1658874981149148No Hit
CGTGGCGGAACATCGAGTGACTTTAGCTGGAAGAAATGTCTGAGAAGCTCTGTATGTGAGGAGG210.15834715729150958No Hit
CGTGGCGGGCAATTCTGTCCCAATCTCACCAGATGCTACTGTACTTGAATGGTTAATAAACTGC190.14326647564469913No Hit
CGTGGCGGGATGTTAGCAGGACTTTTCTAGGAGAGACTTATGTATAATTGCTTTTTAAAATGCA190.14326647564469913No Hit
CGTGGCGGGATGTACATTGCACTTTGATCTGTAACTGAGCGACCCTGGGGTCTGCTATAGAGCT170.1281857939978887No Hit
CGTGGCGGGGTTCAGCTTGCCTTCTTATAGAACCCAGGACCACCAGCCCAGGGATGGCACCTCT160.12064545317448348No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGAGGTTTGCG160.12064545317448348No Hit
CGTGGCGGGATTTGTGGTCACAGTACACAGTGAATTCAGGTTTAAATCATTTTAGGTATTATGG160.12064545317448348No Hit
CGTGGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12064545317448348No Hit
CGTGGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12064545317448348No Hit
CGTGGCTGTGTCTTTGTGGTTAGCTCTCGTTCTTTGGTAGTTTTCATTAAAGCCAATACTTGGT150.11310511235107827No Hit
CGTGGCGGGCATTCCCTGTCTTTTAAGTCCAGCACATTGGGAGACAAACTAAGCAAATCTGGGA150.11310511235107827No Hit
CGTGGCGAGAGATCTTAAAATACGCTGTGGAATTCTGAGTCTGCAATGTCAGGGCTGGGAGGGC150.11310511235107827No Hit
CGTGGCGGGCATCAACACTCCAGGCCCCTGCCTACCCACTTGCTATTGAAGAGGGGGCTTCAGG150.11310511235107827No Hit
CGTGGCGGGATATGGTATATATATAACCACCCTGAACCTTACCCATGCTGTCAAAGATGCCTGG140.10556477152767305No Hit
CGTGGCGGGAACCAAAGATCTGACCAGTCATGGTCTCAATAGCATGGGCACCTGTCTGCTTCTA140.10556477152767305No Hit
CGTGGCGGGACTCAAAGGCAAACTTGAAGAGCAGAAACCAGAAAGAGTAAAGCCTTTTATGACT140.10556477152767305No Hit
CGTGGCGGGGTTCAGACCACCGAAAGCAGTTCCCAGCCACATCCAAAAAAAAAAAAAAAAAAAA140.10556477152767305No Hit
CGTGGCGGGATGTTATTTATATAAATAATTTCTCAAATGGATATTTAAAAAAAAGCTAGTCTGT140.10556477152767305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTA150.00452136158.20532637
TTTGAGA150.00452136158.20532644
AATCCAT150.00452136158.20532652
ATCCATC150.00452136158.20532653
TATGTAT150.00452136158.20532638
GTATTTG150.00452136158.20532641
ACTCTCA150.00452136158.20532644
TTGAGAA150.00452136158.20532645
ATAATCC150.00452136158.20532650
GCTAATA150.00452136158.20532655
AGAAAAT150.00452136158.20532648
TTCTTCC150.00452136158.20532637
CTAATAT150.00452136158.20532656
TTCCACT150.00452136158.20532640
TAATCCA150.00452136158.20532651
TATTTGA150.00452136158.20532642
TGCTAAT150.00452136158.20532654
TAATATA150.00452136158.20532657
ATGTATT150.00452136158.20532639
TCTCATA150.00452136158.20532646
Produced by FastQC (version 0.11.5)