FastQCFastQC Report
Fri 17 Jun 2016
SRR1526432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13497
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGAAAATGCTAATATAT1180.8742683559309476No Hit
CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.4741794472845818No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.40008890864636587No Hit
CGTGGCGGGACATTTAGGTCTATAATGCTTCTAAAACTGGTTTTGTATATGGCAGAGACTATTT450.3334074238719716No Hit
CGTGGCGAGAGAAAAGGCATCGATATATTGATAATCTACTGTACCAAAAGTTGGCATCCGAGAA430.31858931614432834No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGGGGTTTGCG370.2741349929613988No Hit
CGTGGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.22227161591464767No Hit
CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.2148625620508261No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGAGGTTTGCG280.20745350818700453No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.19263540045936134No Hit
CGTGGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.19263540045936134No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGGGGGTTGCG260.19263540045936134No Hit
CGTGGCGGGATCTTTCATCTACCTATCTAAGGCATCTTCTTCCACTCTCATAATCCATCTGTAT240.17781729273171815No Hit
CGTGGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.16299918500407498No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14818107727643182No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14818107727643182No Hit
CGTGGCGGAACATCGAGTGACTTTAGCTGGAAGAAATGTCTGAGAAGCTCTGTATGTGAGGAGG180.13336296954878862No Hit
CGTGGCGGGATTTGTGGTCACAGTACACAGTGAATTCAGGTTTAAATCATTTTAGGTATTATGG170.12595391568496703No Hit
CGTGGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.12595391568496703No Hit
CGTGGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG150.11113580795732383No Hit
CGTGGCGGGACTTATGGGTTTTAAATAAAATACTCCCTGTCTTAAAAAAAAAAAAAAAAAAAAA150.11113580795732383No Hit
CGTGGCGGAGCATGGTATCAACGCAGAGTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11113580795732383No Hit
CGTGGCGGGATGTTATTTATATAAATAATTTCTCAAATGGATATTTAAAAAAAAGCTAGTCTGT150.11113580795732383No Hit
CGTGGCGGGAGCTAGGACAGTAGACAATGCTTTAAATATCAAAAGGGACTTTCAGATACTTTGA140.10372675409350227No Hit
CGTGGCGAGAGATCTTAAAATACGCTGTGGAATTCTGAGTCTGCAATGTCAGGGCTGGGAGGGC140.10372675409350227No Hit
CGTGGCGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCA140.10372675409350227No Hit
CGTGGCGGGATGTTAGCAGGACTTTTCTAGGAGAGACTTATGTATAATTGCTTTTTAAAATGCA140.10372675409350227No Hit
CGTGGCGGGGTGCAGTGCTCGTGGCGGAGATTAAGTAAGTTCTGCATATTTCTAAGTAGCCACA140.10372675409350227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGG150.004360241358.7424239
GAGGCAC201.8546125E-458.52075242
AGAGGCA201.8546125E-458.52075241
GAGAGGC201.8546125E-458.52075240
TGAGAAA257.961882E-658.3007546
GTTTGGG201.8897359E-458.3007549
GTTTGCG150.00449326558.3007558
GGCACGT201.8897359E-458.3007544
CTATACA150.00449326558.3007533
TATACAG150.00449326558.3007534
CACGTTT201.8897359E-458.3007546
GAAAATG257.961882E-658.3007549
CCATGTA201.8897359E-458.3007533
GGTTTGC150.00449326558.3007557
TTGAGAA257.961882E-658.3007545
GCACGTT201.8897359E-458.3007545
CTAATAT257.961882E-658.3007556
ATGCTAA257.961882E-658.3007553
ATTTGAG257.961882E-658.3007543
TAATATA257.961882E-658.3007557
Produced by FastQC (version 0.11.5)