FastQCFastQC Report
Fri 17 Jun 2016
SRR1526431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21574
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.7323630295726337No Hit
CGTGGCGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGAAAATGC1090.5052377862241587No Hit
CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.41716881431352554No Hit
CGTGGCGGGACATTTAGGTCTATAATGCTTCTAAAACTGGTTTTGTATATGGCAGA890.41253360526559746No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.3569110966904607No Hit
CGTGGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.33373505145082044No Hit
CGTGGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT620.2873829609715398No Hit
CGTGGCGGGATGCATACAGTGAGCAGACTTTTCATTCCTCTGGTTCTGTGGTTGTC570.26420691573189947No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGG500.23176045239640305No Hit
CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGA490.22712524334847503No Hit
CGTGGCGGGATCTTTCATCTACCTATCTAAGGCATCTTCTTCCACTCTCATAATCC440.2039491981088347No Hit
CGTGGCGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCA350.16223231667748217No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.1575971076295541No Hit
CGTGGCGGGATTTGTGGTCACAGTACACAGTGAATTCAGGTTTAAATCATTTTAGG280.12978585334198572No Hit
CGTGGCGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT280.12978585334198572No Hit
CGTGGCGAGAGAAAAGGCATCGATATATTGATAATCTACTGTACCAAAAGTTGGCA260.12051543524612959No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11588022619820153No Hit
CGTGGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11588022619820153No Hit
CGTGGCGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT250.11588022619820153No Hit
CGTGGCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTT220.10197459905441734No Hit
CGTGGCGGGCATTCCCTGTCTTTTAAGTCCAGCACATTGGGAGACAAACTAAGCAA220.10197459905441734No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10197459905441734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTGG251.8737792E-550.63120737
AGCTGTA150.0079153650.63120337
TCCTCTG251.9002959E-550.5117936
TTCATTC251.9002959E-550.5117931
GAGCTGT150.00798985150.5117936
CAGAGCT150.00798985150.5117934
AGAGCTG150.00798985150.5117935
TCAGAGC150.00798985150.5117933
CTAAAAC150.00798985150.5117931
TGAATTC150.00798985150.5117931
TCATTCC251.9002959E-550.5117932
CATTTTA203.9361374E-450.39294448
AAATTCG150.00806486150.39293748
AGCAACT150.00806486150.39293748
TCATAAT150.00806486150.39293748
TATGGCA150.00806486150.39293748
TGGTTGT251.9542626E-550.2746549
AAATCAT203.982266E-450.2746544
AATCATT203.982266E-450.2746545
TTTTAGG203.982266E-450.2746550
Produced by FastQC (version 0.11.5)