##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526431_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21574 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.27500695281357 38.0 36.0 38.0 35.0 39.0 2 36.929452118290534 38.0 36.0 38.0 35.0 39.0 3 36.064522109947156 37.0 35.0 38.0 33.0 39.0 4 36.949939742282375 38.0 35.0 38.0 35.0 39.0 5 36.82960971539816 38.0 35.0 38.0 35.0 39.0 6 36.7436265875591 38.0 35.0 38.0 35.0 39.0 7 36.78070825994252 38.0 36.0 38.0 35.0 39.0 8 36.9612960044498 38.0 36.0 38.0 35.0 39.0 9 34.93923240938166 35.0 35.0 36.0 32.0 39.0 10 30.85385185871883 34.0 27.0 35.0 21.0 37.0 11 31.933901918976545 34.0 29.0 38.0 22.0 38.0 12 31.61351626958376 33.0 29.0 36.0 23.0 38.0 13 31.385139519792343 33.0 29.0 36.0 22.0 38.0 14 31.394641698340596 33.0 29.0 36.0 23.0 38.0 15 31.82409381663113 34.0 29.0 36.0 23.0 38.0 16 30.24552702326875 33.0 27.0 36.0 19.0 38.0 17 30.196857328265505 33.0 27.0 36.0 20.0 38.0 18 30.002456660795403 32.0 27.0 36.0 19.0 38.0 19 29.55988690089923 32.0 26.0 35.0 18.0 38.0 20 29.738249745063502 32.0 27.0 36.0 18.0 38.0 21 29.11031797534069 32.0 25.0 36.0 16.0 38.0 22 28.76346528228423 31.0 25.0 35.0 15.0 38.0 23 28.490312413089832 31.0 25.0 35.0 14.0 38.0 24 28.303930657272645 31.0 24.0 35.0 13.0 38.0 25 27.997728747566516 30.0 24.0 35.0 12.0 38.0 26 27.33823120422731 30.0 22.0 35.0 4.0 38.0 27 27.24673217762121 30.0 22.0 35.0 4.0 38.0 28 27.036896264021507 30.0 22.0 35.0 4.0 38.0 29 26.954945768054138 30.0 22.0 35.0 4.0 38.0 30 26.6391489756188 30.0 21.0 35.0 4.0 38.0 31 26.747798275702234 31.0 20.0 36.0 4.0 38.0 32 26.44762213775841 31.0 19.0 35.0 4.0 38.0 33 26.463659961064245 31.0 20.0 35.0 4.0 38.0 34 26.522480763882452 31.0 20.0 35.0 4.0 38.0 35 26.37707425604895 31.0 20.0 35.0 4.0 38.0 36 27.309631964401593 34.0 22.0 35.0 4.0 38.0 37 26.93682210067674 33.0 21.0 35.0 4.0 38.0 38 26.93172337072402 33.0 21.0 35.0 4.0 38.0 39 26.726939834986556 33.0 20.0 35.0 4.0 38.0 40 26.43737832576249 33.0 18.0 35.0 4.0 38.0 41 26.034578659497544 33.0 15.0 35.0 4.0 38.0 42 25.779966626494854 33.0 14.0 35.0 4.0 38.0 43 25.76870306850839 33.0 15.0 35.0 4.0 38.0 44 25.311393343839807 32.0 10.0 35.0 4.0 38.0 45 25.169092426068417 32.0 4.0 35.0 4.0 38.0 46 24.779456753499584 33.0 4.0 35.0 4.0 38.0 47 24.45744878094002 32.0 4.0 35.0 4.0 38.0 48 23.953972374154073 32.0 4.0 35.0 4.0 38.0 49 23.89737647167887 32.0 4.0 35.0 4.0 38.0 50 23.287197552609623 31.0 4.0 35.0 4.0 38.0 51 22.731621396124964 31.0 4.0 35.0 4.0 38.0 52 22.43246500417169 31.0 4.0 35.0 4.0 38.0 53 21.99295448224715 30.0 4.0 35.0 4.0 37.0 54 21.53810141837397 30.0 4.0 35.0 4.0 37.0 55 21.03022156299249 30.0 4.0 35.0 4.0 37.0 56 12.912070084360805 15.0 4.0 21.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.07330527371073003 1 2 0.686988622827613 1 3 1.441724439047519 1 4 -0.35972436538643393 1 5 0.16816526893908446 1 6 0.4151740056185744 1 7 0.5671541933955666 1 8 0.4716344192312647 1 9 0.24610565497840753 1 10 -1.3234255457233353 1 11 2.0378328859888164 1 12 -0.05465324909059177 1 13 2.0593341047413283 1 14 0.5811963477518205 1 15 1.7656557606371202 1 16 3.724592681185804 1 17 3.459658447694764 1 18 2.5224095379865332 1 19 3.821129707551844 1 20 1.5466861799761098 1 21 1.2513596574588775 1 22 0.8030004690143073 1 23 1.5561984573895806 1 24 1.0631162357142898 1 25 0.0893019734446554 1 26 2.100535747184928 1 27 -0.8600110626185362 1 28 2.1142805946315804 1 29 1.2019762354384618 1 30 2.641824361288794 1 31 0.06120001178706502 1 32 0.5691228588753035 1 33 0.13763226465147582 1 34 -0.622932455119777 1 35 -0.0605598115948105 1 36 0.8183738056770729 1 37 1.5108778351851129 1 38 1.557983614168915 1 39 -0.6153473217697076 1 40 1.1368501481735969 1 41 0.22259545413755077 1 42 0.5753549570008296 1 43 1.0605047007939383 1 44 1.0127754200806613 1 45 0.9067193179973003 1 46 -2.766398218248188 1 47 -0.48031915780435597 1 48 0.47289843418124633 1 49 0.4565702940417182 1 50 0.19777123603121893 1 51 1.4948083324527879 1 52 2.4369693236059327 1 53 3.3561594522988187 1 54 2.452399003475488 1 55 2.0720712320825037 1 56 1.6126347062557755 2 1 0.19224496552372017 2 2 0.7850747472295296 2 3 0.8890928601001491 2 4 0.6570220460968201 2 5 0.8380217282692328 2 6 0.39842759413532036 2 7 0.7035178297591997 2 8 0.4118258067910716 2 9 0.8130913009114167 2 10 -0.05787530648888861 2 11 -3.304272377169081 2 12 -0.9517824356934597 2 13 0.63110443966956 2 14 0.05727290277574326 2 15 -4.8061145742911116 2 16 -3.4715795676180257 2 17 -2.32263820302294 2 18 -1.8818966821091578 2 19 -3.750640627376388 2 20 -1.8982898965789161 2 21 -1.6170613951727013 2 22 -1.8214014448612907 2 23 -0.8983469971558726 2 24 -1.0923861566302087 2 25 -0.2958654906701774 2 26 -1.0095120997050238 2 27 0.6447736263766792 2 28 -0.35222658240191507 2 29 0.5081963311322397 2 30 -0.8246828157447013 2 31 -1.5057856341459512 2 32 -0.16293455739263862 2 33 0.42471360436439554 2 34 -0.23537264650733647 2 35 0.4203014324243277 2 36 -0.8993295436052229 2 37 -1.6733326911306783 2 38 -0.02096375425213637 2 39 -0.7875961255974602 2 40 1.2851755070252722 2 41 -2.2152035889246484 2 42 -2.778712028645103 2 43 -3.0471508015984057 2 44 -2.2192819961872807 2 45 -3.2439983853519827 2 46 -2.0463025244682846 2 47 -3.0855823156990922 2 48 -1.2256661591201912 2 49 -1.3640038686377096 2 50 -1.0151474242558614 2 51 -1.6535170263988874 2 52 -0.7831263701739708 2 53 -1.3591515524858693 2 54 -1.1074096089647014 2 55 -1.526014892319413 2 56 -1.4830590736485298 3 1 -0.1713913981126467 3 2 0.42143838359316277 3 3 0.43454740555469584 3 4 0.38429477336954676 3 5 0.7471126373601393 3 6 0.125700321408047 3 7 0.6580632843046601 3 8 0.45728035224561836 3 9 0.2221822100023303 3 10 1.1239428753292948 3 11 2.1502730773763723 3 12 0.6391266552156303 3 13 0.44928625785137655 3 14 -0.35181800631516325 3 15 0.9211581529816151 3 16 0.16478406874561102 3 17 2.4046345242497864 3 18 0.39083059061811554 3 19 -1.3415497182854779 3 20 0.19261919433017738 3 21 -2.2988795769908847 3 22 -2.275946899406744 3 23 0.056198457389580625 3 24 -2.728749792993842 3 25 -2.477683672488361 3 26 -2.782239372432297 3 27 -1.1734081918051409 3 28 -2.352226582401915 3 29 -1.9008945779586703 3 30 -2.7337737248356078 3 31 -0.5512401796004944 3 32 -2.617480011938092 3 33 -1.3480136683628778 3 34 -0.2808271919618832 3 35 -0.7615167493938522 3 36 -3.3538749981506797 3 37 -2.900605418403405 3 38 -1.7936910269794097 3 39 -1.8330506710520034 3 40 -3.4420972202474545 3 41 -2.260658134379195 3 42 -3.2332574831905596 3 43 -4.774423528871136 3 44 -4.128372905278191 3 45 -2.698543839897436 3 46 -4.5008479790137415 3 47 -3.9946732247900023 3 48 -3.9983934318474645 3 49 -2.9094584140922564 3 50 -3.3333292424376815 3 51 -2.789880662762524 3 52 -0.828580915628514 3 53 -1.7227879161222326 3 54 -2.0165005180556115 3 55 0.473985107680587 3 56 -2.2103318009212582 4 1 0.5407298140085643 4 2 1.4062868684416472 4 3 0.6748071458144338 4 4 1.001177890252663 4 5 0.12806501831251893 4 6 1.1495098452175725 4 7 1.233820860062231 4 8 1.128276023241284 4 9 0.4040003918205102 4 10 0.8620381134245321 4 11 0.8147752418785394 4 12 0.6997327158216926 4 13 1.6332689418340642 4 14 2.405757751260591 4 15 0.2393399711634352 4 16 0.7621866661482066 4 17 0.6275782471935116 4 18 1.5574972572847834 4 19 1.5805282037924435 4 20 2.569242570953552 4 21 2.692462414351109 4 22 2.319291195831351 4 23 1.2228651240562485 4 24 2.6846701204260697 4 25 2.3599786651739763 4 26 5.245899155706233 4 27 4.6036480852511374 4 28 1.3598946297192995 4 29 2.9237807467166554 4 30 1.0302955392336557 4 31 2.7604481320878165 4 32 1.438797044338962 4 33 2.8619430415938325 4 34 2.8858394747047846 4 35 2.0371845493074474 4 36 0.5552159109402304 4 37 1.7314292136312268 4 38 -0.7352494685378517 4 39 0.9310185930172601 4 40 0.8436170654668302 4 41 -0.4641213378423963 4 42 -0.7830410329741078 4 43 0.175792921345316 4 44 -0.2604075373128225 4 45 0.03955139819780129 4 46 -1.0095059876717478 4 47 -0.20462993474671265 4 48 -1.4594323928864235 4 49 -1.7514497560835984 4 50 -1.9826798917883295 4 51 1.168993796111934 4 52 1.108648521600923 4 53 3.3486406553063404 4 54 1.1090406074855146 4 55 3.352772986468466 4 56 1.0061184155289595 5 1 0.10133587461462668 5 2 0.9214383835931628 5 3 0.11636558737287572 5 4 0.38429477336954676 5 5 0.6107490009965062 5 6 0.21660941231714048 5 7 0.5216996479410199 5 8 0.7300076249728917 5 9 -0.09599960817948983 5 10 1.7148519662383883 5 11 -1.1679087408054443 5 12 0.5482175643065368 5 13 1.7674680760331931 5 14 2.19363653913938 5 15 0.28479451661798194 5 16 0.7102386142001542 5 17 1.4500890697043332 5 18 1.9362851360726623 5 19 1.0220866453508854 5 20 2.6017101034210874 5 21 1.5647567866454821 5 22 -0.8668559903158339 5 23 2.7834711846623073 5 24 2.953068388824338 5 25 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2.0739428753292977 6 11 1.8457276228309176 6 12 2.916399382488354 6 13 2.1856498942150147 6 14 -1.310908915406074 6 15 1.1393399711634338 6 16 1.6693295232910614 6 17 0.6728163424316058 6 18 0.24083059061811696 6 19 1.6448139180781602 6 20 0.6335282852392687 6 21 1.832938604827298 6 22 1.7740531005932567 6 23 2.5561984573895806 6 24 0.08943202518797477 6 25 3.081407236602548 6 26 0.9768515366586144 6 27 -0.14159000998695603 6 28 1.4432279630526317 6 29 2.4309236038595117 6 30 0.7207717297098455 6 31 2.903305274944959 6 32 2.150701806243724 6 33 0.9428954225462114 6 34 2.2691728080381175 6 35 2.015755977878875 6 36 2.0552159109402304 6 37 -0.17333269113067828 6 38 2.929036245747863 6 39 3.1714947834934506 6 40 2.457902779752544 6 41 1.1120691383480796 6 42 1.5121970622639864 6 43 2.0710310165834116 6 44 2.994354367449084 6 45 2.801456160102564 6 46 2.0809702028044406 6 47 2.1644176843009078 6 48 2.0834247499707175 6 49 3.572359767725928 6 50 3.5030343939259545 6 51 1.2737557008738385 6 52 1.6896009025533019 6 53 2.0272120838777674 6 54 2.847135845580752 6 55 1.6194396531351352 6 56 0.3442136536241964 7 1 0.5883488616276153 7 2 1.2158106779654574 7 3 0.7700452410525287 7 4 0.8107016997764731 7 5 1.128065018312519 7 6 0.7209384166461419 7 7 1.186201812443187 7 8 0.9854188803841453 7 9 1.4516194394395612 7 10 3.1001333515197693 7 11 1.0528704799737731 7 12 0.03306604915502476 7 13 0.20469751326263363 7 14 -1.0704327249298835 7 15 1.382197114020581 7 16 2.4288533328148745 7 17 1.8656734852887524 7 18 1.843211542999068 7 19 0.9138615371257792 7 20 -0.04980504809406483 7 21 2.501986223874919 7 22 -0.061661185121028694 7 23 2.794293695484818 7 24 2.0656225013784493 7 25 3.502835808031122 7 26 3.1030420128490874 7 27 1.7941242757273272 7 28 2.5503708201954893 7 29 2.8285426514785605 7 30 1.69696220590032 7 31 2.808067179706864 7 32 0.6292732348151517 7 33 1.90956208921288 7 34 1.0763156651809744 7 35 2.227660739783637 7 36 3.079025434749756 7 37 3.160000642202654 7 38 3.2647505314621483 7 39 3.7881614501601177 7 40 3.081712303562071 7 41 3.0596881859671257 7 42 3.597911347978272 7 43 2.7472214927738854 7 44 4.406259129353845 7 45 3.0871704458168487 7 46 3.990494012328252 7 47 3.8906081604913823 7 48 2.778662845208814 7 49 1.9628359582021169 7 50 2.493510584402145 7 51 3.4070890342071714 7 52 3.108648521600923 7 53 3.4438787505444353 7 54 3.1090406074855146 7 55 3.4003920340875133 7 56 1.2442136536241968 8 1 -0.12593685265809995 8 2 0.5729535351083186 8 3 0.5974261934334848 8 4 0.2214159854907578 8 5 0.5387793040268036 8 6 0.125700321408047 8 7 0.6921541933955666 8 8 0.49137126133652487 8 9 1.2373337251538459 8 10 3.0822762086626305 8 11 1.5290609561642547 8 12 0.9497327158216926 8 13 1.043983227548349 8 14 -0.2609089154060733 8 15 0.9893399711634352 8 16 -0.7556704767089357 8 17 -2.7771836575683935 8 18 -1.4425027427152166 8 19 -0.621852748588509 8 20 -1.1331383814274005 8 21 -2.033728061839369 8 22 -3.317613566073412 8 23 0.13953179072291277 8 24 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1.1829386048272994 118 22 0.5240531005932567 118 23 0.2061984573895792 118 24 0.23943202518797335 118 25 1.1814072366025492 118 26 3.626851536658613 118 27 3.2584099900130425 118 28 3.893227963052631 118 29 3.1809236038595117 118 30 2.7207717297098455 118 31 4.50330527494496 118 32 2.800701806243726 118 33 2.6428954225462107 118 34 1.1191728080381154 118 35 1.9157559778788738 118 36 0.45521591094022895 118 37 1.4266673088693231 118 38 0.7290362457478636 118 39 1.5714947834934492 118 40 1.157902779752547 118 41 1.2620691383480782 118 42 2.112197062263988 118 43 2.521031016583411 118 44 3.1443543674490826 118 45 1.5014561601025633 118 46 4.180970202804442 118 47 4.4644176843009085 118 48 3.3334247499707175 118 49 2.322359767725928 118 50 4.303034393925955 118 51 1.62375570087384 118 52 1.9896009025533026 118 53 2.127212083877769 118 54 1.1471358455807525 118 55 1.7694396531351337 118 56 0.7942136536241975 119 1 0.39787267115142555 119 2 0.6920011541559319 119 3 0.9605214315287185 119 4 0.4773683664431445 119 5 1.17568406593157 119 6 0.3399860356937623 119 7 0.9004875267289023 119 8 0.6997045946698606 119 9 0.7373337251538459 119 10 -0.6141523627659424 119 11 1.6719180990213935 119 12 0.03306604915502476 119 13 1.823745132310254 119 14 -1.7370993915965478 119 15 -0.3797076478841852 119 16 2.2383771423386847 119 17 0.9132925329077963 119 18 1.5574972572847834 119 19 1.9138615371257792 119 20 -0.9069479052369225 119 21 2.597224319113014 119 22 2.319291195831351 119 23 1.0800079811991061 119 24 0.9227653585213069 119 25 -0.3543070491117355 119 26 0.2935182033252808 119 27 -2.1582566766536253 119 28 -1.7829625131378428 119 29 0.11425693719284524 119 30 -2.636371127433012 119 31 1.236638608278291 119 32 1.7721303776722976 119 33 1.9571811368319274 119 34 4.123934712800022 119 35 2.7038512159741117 119 36 2.6504540061783253 119 37 5.0171434993455115 119 38 3.6457029124145315 119 39 3.8833995453982126 119 40 2.700759922609688 119 41 1.8215929478718884 119 42 2.502673252740177 119 43 0.604364349916743 119 44 3.5491162722109877 119 45 2.6585990172454217 119 46 3.84763686947111 119 47 4.033465303348528 119 48 2.1596152261611934 119 49 2.8199788153449745 119 50 3.874462965354528 119 51 4.121374748492887 119 52 5.108648521600923 119 53 5.015307321973005 119 54 4.2042787027236095 119 55 5.067058700754181 119 56 2.053737463148007 120 1 0.024063147341898627 120 2 0.6896202017749857 120 3 -0.16090713989984806 120 4 -0.5702506811759065 120 5 0.5471126373601365 120 6 0.4757003214080484 120 7 0.5171541933955695 120 8 0.26637126133652345 120 9 0.004000391820511595 120 10 -1.0760571246707045 120 11 -0.4042723771690824 120 12 -0.5836006175116424 120 13 -1.0643501057849853 120 14 0.039091084593927405 120 15 -1.710660028836564 120 16 -2.5306704767089343 120 17 -2.2271836575683928 120 18 -2.709169409381886 120 19 -1.005186081921842 120 20 -3.416471714760732 120 21 -1.3670613951727013 120 22 -0.12594689940674542 120 23 -0.34380154261041795 120 24 -2.710567974812026 120 25 -2.8685927633974515 120 26 -1.3231484633413864 120 27 -0.09159000998695888 120 28 -0.2067720369473669 120 29 -1.1690763961404862 120 30 -2.479228270290154 120 31 0.9533052749449595 120 32 1.650701806243724 120 33 1.1428954225462107 120 34 2.5191728080381175 120 35 0.5157559778788752 120 36 0.955215910940229 120 37 0.2266673088693203 120 38 2.079036245747865 120 39 1.4214947834934506 120 40 1.8579027797525463 120 41 0.5620691383480789 120 42 0.9121970622639886 120 43 2.3210310165834116 120 44 2.994354367449084 120 45 0.9514561601025626 120 46 1.1309702028044413 120 47 0.7144176843009085 120 48 1.933424749970719 120 49 2.1223597677259285 120 50 2.053034393925955 120 51 1.4237557008738406 120 52 1.089600902553304 120 53 0.677212083877766 120 54 1.0471358455807511 120 55 1.969439653135133 120 56 -0.7057863463758025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 81.0 5 21.0 6 13.0 7 36.0 8 163.0 9 100.0 10 93.0 11 202.0 12 203.0 13 247.0 14 358.0 15 370.0 16 405.0 17 408.0 18 375.0 19 348.0 20 334.0 21 412.0 22 439.0 23 506.0 24 536.0 25 624.0 26 742.0 27 912.0 28 1078.0 29 1251.0 30 1428.0 31 1655.0 32 1845.0 33 1815.0 34 1591.0 35 1096.0 36 796.0 37 702.0 38 317.0 39 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.91062488410903 0.74633784535509 0.2595957722974226 0.08344149823845726 8 98.6279781218133 1.0290164086400295 0.1807731528691944 0.16223231667748217 9 94.89663483823121 3.8472235097802914 0.7323630295726337 0.5237786224158709 10 23.106517103921387 60.84638917215166 3.596922221192176 12.450171502734774 11 23.917678687308797 30.986372485399095 29.72559562436266 15.37035320292945 12 41.7400574765922 18.397144711226478 18.726244553629368 21.13655325855196 13 20.051914341336794 14.49429869287105 38.676184295911746 26.777602669880412 14 22.47149346435524 30.453323444887364 28.35820895522388 18.716974135533512 15 30.15203485677204 28.529711689997217 21.89672754241216 19.421525910818577 16 30.101047557244833 27.514600908500974 21.78084731621396 20.603504218040232 17 28.538982108093077 26.59219430796329 22.986001668675257 21.882821915268377 18 25.901548159822006 27.491424863261333 25.164549921201445 21.442477055715212 19 22.638360990080653 29.123018448132008 26.670992861778064 21.56762770000927 20 24.075275794938353 25.804208769815517 26.08695652173913 24.033558913507 21 23.806433670158526 25.906183368869932 27.829795123760082 22.45758783721146 22 22.105311949568925 25.206266802632797 27.890052841383145 24.79836840641513 23 24.636136089737647 26.35116343747103 28.534346899045147 20.478353573746176 24 23.083341058681746 28.543617317141003 27.95031055900621 20.42273106517104 25 23.350489173273985 28.390596745027125 26.308712384661753 21.950201697037137 26 24.71248376924504 27.026525690966423 27.490261547022815 20.770728992765722 27 20.59846903270703 24.022268615170496 30.447691950823476 24.931570401299002 28 20.2467646922399 25.131035762326636 32.49686905700636 22.125330488427107 29 23.098696116189505 22.73212379935966 33.7385736160735 20.430606468377338 30 20.782237086584914 24.707357859531772 35.976402824228906 18.534002229654405 31 18.63997398857355 24.390357192623902 38.445817269729204 18.523851549073342 32 19.166240646930337 22.88423107310499 40.2193614351443 17.730166844820374 33 19.130434782608695 22.85514996512439 39.95349918623576 18.060916066031155 34 15.900320795945882 25.18480636012832 43.47947370868009 15.435399135245712 35 15.95200446470096 27.155613431308716 41.363594084271234 15.528788019719094 36 15.353121801432959 24.704568716851213 43.360937936168234 16.581371545547594 37 17.421750164489143 24.264498543096156 42.715480778268635 15.598270514146067 38 16.625541226314073 24.6473299501839 43.544857768052516 15.182271055449508 39 17.410735068199802 25.05935477864159 43.38717936781342 14.14273078534519 40 17.6857422694837 23.846835502075464 42.75919966419477 15.708222564246071 41 16.4593524341952 24.07640344747263 44.2254833449802 15.238760773351967 42 17.03555286333349 23.871208238199525 43.991426308186945 15.101812590280042 43 16.598265087212013 24.610577371513852 42.02033392407425 16.770823617199888 44 17.4316634014367 26.31775352178375 41.263177535217835 14.987405541561714 45 16.776300443200373 25.164450664800558 42.47259155586657 15.586657336132493 46 15.759018152970276 25.530822716878998 41.35517289654207 17.35498623360866 47 16.448903406439573 26.929538030797946 40.80261315912273 15.81894540363976 48 16.486082570521205 27.367831122734916 41.69624509620773 14.44984121053615 49 17.823122898767277 26.9704893537542 39.55453866268211 15.651849084796412 50 16.174067329691365 27.963767100901155 41.3830134939534 14.47915207545408 51 15.496311513680084 28.392006723316836 42.2681856382482 13.843496124754878 52 17.44712638311779 26.915355525468044 41.15504925533405 14.482468836080114 53 17.62096020922847 27.20437138053428 40.40724827199701 14.76742013824024 54 19.88880583068585 28.106895907307045 38.19846757615399 13.805830685853113 55 18.52457484582321 26.83610540085965 39.207624743038686 15.431695010278453 56 17.208019815862034 28.279665373650513 37.813712202645235 16.69860260784222 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 3.0 10 3.5 11 4.0 12 5.5 13 24.0 14 41.0 15 49.5 16 58.0 17 65.5 18 73.0 19 94.5 20 116.0 21 218.0 22 311.0 23 302.0 24 326.0 25 350.0 26 403.0 27 456.0 28 626.5 29 856.0 30 915.0 31 961.0 32 1007.0 33 1091.0 34 1175.0 35 1197.0 36 1219.0 37 1102.0 38 863.0 39 741.0 40 813.0 41 885.0 42 804.5 43 724.0 44 681.0 45 638.0 46 658.0 47 753.0 48 828.0 49 810.5 50 793.0 51 802.0 52 811.0 53 756.5 54 730.5 55 759.0 56 752.5 57 746.0 58 791.5 59 837.0 60 809.0 61 781.0 62 737.0 63 632.5 64 572.0 65 576.0 66 580.0 67 546.0 68 512.0 69 445.0 70 378.0 71 366.5 72 280.0 73 205.0 74 178.5 75 152.0 76 142.5 77 133.0 78 105.5 79 69.5 80 61.0 81 42.5 82 24.0 83 18.5 84 13.0 85 13.5 86 14.0 87 18.5 88 16.5 89 10.0 90 8.0 91 6.0 92 3.5 93 1.0 94 1.0 95 1.0 96 2.5 97 6.0 98 8.0 99 4.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.009270418095856122 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03244646333549643 26 0.046352090479280614 27 0.08806897191063318 28 0.06952813571892093 29 0.10660980810234541 30 0.21321961620469082 31 0.20858440715676277 32 0.26420691573189947 33 0.31982942430703626 34 0.301288588115324 35 0.33373505145082044 36 0.3708167238342449 37 1.3720218781867062 38 0.4403448595531659 39 0.4310744414573097 40 0.6164828033744322 41 0.5052377862241587 42 0.5237786224158709 43 0.6118475943265042 44 0.6303884305182164 45 0.6442940576620005 46 0.672105311949569 47 0.6674701029016409 48 0.750903865764346 49 0.7323630295726337 50 0.7277278205247057 51 0.7230926114767776 52 0.7184574024288496 53 0.750903865764346 54 0.7879855381477705 55 0.7879855381477705 56 0.8204320014832669 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 21574.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.57754704737184 #Duplication Level Percentage of deduplicated Percentage of total 1 77.63409595225342 47.02883100027812 2 10.765934654525978 13.043478260869565 3 4.453286402938251 8.093074997682395 4 2.41028387787895 5.840363400389357 5 1.4079118524753231 4.264392324093817 6 1.0100237202540363 3.6710855659590242 7 0.6197872828831585 2.628163530175211 8 0.4131915219221058 2.0024103087049228 9 0.2065957609610529 1.126355798646519 >10 1.0176754151044456 8.99230555298044 >50 0.0459101691024562 2.0719384444238433 >100 0.01530338970081873 1.2376008157967924 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 158 0.7323630295726337 No Hit CGTGGCGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGAAAATGC 109 0.5052377862241587 No Hit CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.41716881431352554 No Hit CGTGGCGGGACATTTAGGTCTATAATGCTTCTAAAACTGGTTTTGTATATGGCAGA 89 0.41253360526559746 No Hit CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77 0.3569110966904607 No Hit CGTGGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.33373505145082044 No Hit CGTGGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 62 0.2873829609715398 No Hit CGTGGCGGGATGCATACAGTGAGCAGACTTTTCATTCCTCTGGTTCTGTGGTTGTC 57 0.26420691573189947 No Hit CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGG 50 0.23176045239640305 No Hit CGTGGCGGGCAATGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGA 49 0.22712524334847503 No Hit CGTGGCGGGATCTTTCATCTACCTATCTAAGGCATCTTCTTCCACTCTCATAATCC 44 0.2039491981088347 No Hit CGTGGCGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCA 35 0.16223231667748217 No Hit CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.1575971076295541 No Hit CGTGGCGGGATTTGTGGTCACAGTACACAGTGAATTCAGGTTTAAATCATTTTAGG 28 0.12978585334198572 No Hit CGTGGCGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 28 0.12978585334198572 No Hit CGTGGCGAGAGAAAAGGCATCGATATATTGATAATCTACTGTACCAAAAGTTGGCA 26 0.12051543524612959 No Hit CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.11588022619820153 No Hit CGTGGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.11588022619820153 No Hit CGTGGCGGGAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGAAGGAAT 25 0.11588022619820153 No Hit CGTGGCGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTT 22 0.10197459905441734 No Hit CGTGGCGGGCATTCCCTGTCTTTTAAGTCCAGCACATTGGGAGACAAACTAAGCAA 22 0.10197459905441734 No Hit CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.10197459905441734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.004635209047928061 0.0 0.0 0.0 0.0 11 0.004635209047928061 0.0 0.0 0.0 0.0 12 0.004635209047928061 0.0 0.0 0.0 0.0 13 0.004635209047928061 0.0 0.0 0.0 0.0 14 0.018540836191712245 0.0 0.0 0.0 0.0 15 0.023176045239640307 0.0 0.0 0.0 0.0 16 0.02781125428756837 0.0 0.0 0.0 0.0 17 0.02781125428756837 0.0 0.0 0.0 0.0 18 0.02781125428756837 0.0 0.0 0.0 0.0 19 0.02781125428756837 0.0 0.0 0.0 0.0 20 0.03708167238342449 0.0 0.0 0.0 0.0 21 0.03708167238342449 0.0 0.0 0.0 0.0 22 0.046352090479280614 0.0 0.0 0.0 0.0 23 0.05098729952720868 0.0 0.0 0.0 0.0 24 0.0602577176230648 0.0 0.0 0.0 0.0 25 0.06952813571892093 0.0 0.0 0.0 0.0 26 0.06952813571892093 0.0 0.0 0.0 0.0 27 0.06952813571892093 0.0 0.0 0.0 0.0 28 0.06952813571892093 0.0 0.0 0.0 0.0 29 0.06952813571892093 0.0 0.0 0.0 0.0 30 0.06952813571892093 0.0 0.0 0.0 0.0 31 0.06952813571892093 0.0 0.0 0.0 0.0 32 0.07879855381477704 0.0 0.0 0.0 0.0 33 0.07879855381477704 0.0 0.0 0.0 0.0 34 0.07879855381477704 0.0 0.0 0.0 0.0 35 0.08806897191063318 0.0 0.0 0.0 0.0 36 0.09270418095856123 0.0 0.0 0.0 0.0 37 0.09270418095856123 0.0 0.0 0.0 0.0 38 0.10197459905441736 0.0 0.0 0.0 0.0 39 0.10660980810234541 0.0 0.0 0.0 0.0 40 0.10660980810234541 0.0 0.0 0.0 0.0 41 0.10660980810234541 0.0 0.0 0.0 0.0 42 0.10660980810234541 0.0 0.0 0.0 0.0 43 0.10660980810234541 0.0 0.0 0.0 0.0 44 0.12515064429405767 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTCTGG 25 1.8737792E-5 50.631207 37 AGCTGTA 15 0.00791536 50.631203 37 TCCTCTG 25 1.9002959E-5 50.51179 36 TTCATTC 25 1.9002959E-5 50.51179 31 GAGCTGT 15 0.007989851 50.51179 36 CAGAGCT 15 0.007989851 50.51179 34 AGAGCTG 15 0.007989851 50.51179 35 TCAGAGC 15 0.007989851 50.51179 33 CTAAAAC 15 0.007989851 50.51179 31 TGAATTC 15 0.007989851 50.51179 31 TCATTCC 25 1.9002959E-5 50.51179 32 CATTTTA 20 3.9361374E-4 50.392944 48 AAATTCG 15 0.008064861 50.392937 48 AGCAACT 15 0.008064861 50.392937 48 TCATAAT 15 0.008064861 50.392937 48 TATGGCA 15 0.008064861 50.392937 48 TGGTTGT 25 1.9542626E-5 50.27465 49 AAATCAT 20 3.982266E-4 50.27465 44 AATCATT 20 3.982266E-4 50.27465 45 TTTTAGG 20 3.982266E-4 50.27465 50 >>END_MODULE