FastQCFastQC Report
Fri 17 Jun 2016
SRR1526430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152873
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7100.4644378013122003No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6350.4153774701876721No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5980.3911743734995715No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5380.351926108599949No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4600.3009033642304397No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4320.28258750727728243No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3790.24791820661594918No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3620.23679786489438945No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC3390.22175269668286748No Hit
ATCTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3210.2099782172129807No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3150.20605339072301845No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3050.19951201323974802No Hit
ATCTCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.17727132979662857No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2350.15372237085685503No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.1497975443668928No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2260.14783513112191166No Hit
ATCTCTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC2260.14783513112191166No Hit
ATCTCTGGGCCGGGAAGAGTGGCGTTCCTTCCTGCTCAGCTCAGGGGCGTCCCTCCGGACCCTA2100.13736892714867896No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1980.12951927416875447No Hit
ATCTCTGGGGGGCTGGCGAGATGGCTCTGTGGTTAAGAGTGCCGACTGCTCTTCCGAAGGTCCC1880.12297789668548403No Hit
ATCTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1880.12297789668548403No Hit
ATCTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1780.1164365192022136No Hit
ATCTCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1680.10989514171894318No Hit
ATCTCTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1650.10793272847396204No Hit
ATCTCTGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC1650.10793272847396204No Hit
ATCTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA1590.10400790198399978No Hit
ATCTCTGGGATAGCACAAGCTCATCCGTACAGTGTCGATGATTGCGTTGGCTGAGATATGATGC1570.10269962648734571No Hit
ATCTCTGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA1550.10139135099069162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGT201.9685293E-458.21461555
CTATAGT150.00461149658.21461557
TCTAGAT150.00461149658.21461558
GTTGTTA201.9685293E-458.21461557
AGCTATA150.00461149658.21461555
AGTGCGT351.5859769E-858.1954652
ATATCGA150.00462955958.15718550
GATATCG150.00462955958.15718549
GCATGAC150.00462955958.15718550
CGATATC150.00462955958.15718548
CTCGTTT150.00464767558.0998742
CCAACTA150.00464767558.0998742
TTCCCGC150.004653725358.08078838
GACGGTA150.004653725358.08078837
CGTCACG258.562747E-658.08078837
GTAAAAG150.00467798558.00459334
CTTCGTT150.004684064557.98557331
GTTTGCG150.004684064557.98557330
TCGTTGT150.004684064557.98557333
GTGTCGA202.0072884E-457.98557332
Produced by FastQC (version 0.11.5)