FastQCFastQC Report
Fri 17 Jun 2016
SRR1526429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146528
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6190.42244485695566714No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5440.3712601004586154No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4650.3173454902817209No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4330.2955066608429788No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3540.24159205066608427No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3530.2409095872461236No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3460.23613234330639876No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3260.22248307490718497No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2540.17334570867001528No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC2440.1665210744704084No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2390.16310875737060493No Hit
ATCTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2280.15560165975103735No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2010.1371751474120987No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1980.13512775715221664No Hit
ATCTCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.12079602533304214No Hit
ATCTCTGGGCCGGGAAGAGTGGCGTTCCTTCCTGCTCAGCTCAGGGGCGTCCCTCCGGACCCTA1750.11943109849312077No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.11806617165319938No Hit
ATCTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA1680.11465385455339595No Hit
ATCTCTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC1670.11397139113343525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG150.004585649458.29596336
CCCGTTG201.9613675E-458.2557737
CCCTTAC201.9613675E-458.2557739
TGTCGAT201.964714E-458.23570333
CCGTTGT201.964714E-458.23570338
TTATCAA258.443885E-658.2156455
CCCCGTC150.004610762558.2156458
CTTATCA258.443885E-658.2156454
TAAGGCC201.968065E-458.2156456
AAAGGTT201.968065E-458.2156457
GTAAGGC201.968065E-458.2156455
GTAGGTA150.00461705758.19559552
TAACCGA150.00461705758.19559553
TGAGATA201.9714206E-458.1955952
ACGGTAA150.00462335758.1755649
AACGGTA150.004629664658.15553748
CCAATTT258.513134E-658.13553246
CGATTTC150.00463597858.13553246
ACGATTT150.00463597858.13553245
ATGAGTC150.00463597858.13553231
Produced by FastQC (version 0.11.5)