FastQCFastQC Report
Fri 17 Jun 2016
SRR1526428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135499
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11650.8597849430623105No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7830.5778640432770722No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC7260.5357973121572853No Hit
ATCTCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG4400.32472564373168805No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4080.3011092332784744No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC3470.2560904508520358No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT3390.2501863482387324No Hit
ATCTCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2970.21918980951888944No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2910.21476173255891187No Hit
ATCTCTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2710.20000147602565332No Hit
ATCTCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.19409737341234992No Hit
ATCTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2630.19409737341234992No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2420.17859910405242843No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2390.17638506557243963No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2350.17343301426578792No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2320.17121897578579917No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2290.16900493730581037No Hit
ATCTCTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1960.14465051402593376No Hit
ATCTCTGGGCCGGGAAGAGTGGCGTTCCTTCCTGCTCAGCTCAGGGGCGTCCCTCC1890.13948442423929328No Hit
ATCTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC1890.13948442423929328No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1720.12693820618602353No Hit
ATCTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1650.12177211639938301No Hit
ATCTCTGGGGGGCTGGCGAGATGGCTCTGTGGTTAAGAGTGCCGACTGCTCTTCCG1630.12029609074605717No Hit
ATCTCTGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCAT1580.11660602661274252No Hit
ATCTCTGGGACTTAGAGACCTGGCCGGTGCCCTGCGCGGGGCCTCCCCAGGACACA1560.11513000095941667No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC1500.1107019239994391No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC1490.1099639111727762No Hit
ATCTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1480.10922589834611326No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA1430.10553583421279862No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1380.10184577007948399No Hit
ATCTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT1370.10110775725282106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAA203.913589E-450.64836535
TAAAGGC451.1823431E-1050.64836534
GTATAGG150.00799693450.6483634
CGCTTAG150.00799693450.6483634
ACGCTTA150.00805657150.55330333
CGAGTCA251.9508563E-550.53433632
CTGTATA150.00806853850.53433232
CGGGTAC309.773503E-750.45859531
GTACGCT150.00811653950.45859531
TCTGATA204.0761012E-450.2327549
TAAGGGG252.0212348E-550.2327549
TGCATGC150.00827400950.2140247
CGATCCA204.0986712E-450.17660546
ATGTAAG204.1062167E-450.15791339
AGACACG204.1062167E-450.15791339
GATGCAT150.00831066950.15791345
CCCTTAC204.1062167E-450.15791339
ATATTCA150.00831066950.15791339
GTGCCGA204.1062167E-450.15791339
CCAGATT252.0481632E-550.12058342