FastQCFastQC Report
Fri 17 Jun 2016
SRR1526427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107128
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7580.7075647823164812No Hit
ATGGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2080.19416025688895527No Hit
ATGGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1560.14562019266671644No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.14281980434620267No Hit
ATGGCGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCC1360.1269509371966246No Hit
ATGGCGGGGACCAAGCAGAGCCAAACACCTGTGCCGCCGCACAGGATGAGAGCAACATCAAGAA1120.10454783063251437No Hit
ATGGCGGGGGGCACAGGACCACTTCTGCCCAACTCTTTCCACAAAGCCAGGAGCTTCAGAGCAG1100.1026809050855052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAGC150.004627826658.15063550
TCGTTCG201.9765625E-458.15063546
GTATTCT150.004627826658.15063540
GCTATTG201.9765625E-458.15063549
TAAACGC150.004627826658.15063547
TAAGTCC150.004627826658.15063548
TGAGTCG406.9303496E-1058.15063547
TCTAGCT201.9765625E-458.15063545
ATACTAT150.004627826658.15063545
AGAGTAA150.004627826658.15063548
ATTCCCG150.004627826658.15063544
AAACGCA150.004627826658.15063548
CGTAGAG201.9765625E-458.15063542
TCTCGAT150.004627826658.15063543
AACGCAC150.004627826658.15063549
CGTACTG406.9303496E-1058.15063542
CTACCTA150.004627826658.15063549
TCCCGAA303.6629353E-758.15063555
TGCTAAG150.004627826658.15063546
ACGTAGA201.9765625E-458.15063541
Produced by FastQC (version 0.11.5)