FastQCFastQC Report
Fri 17 Jun 2016
SRR1526426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109834
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6670.6072800772074222No Hit
ATGGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1800.16388367900650072No Hit
ATGGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1340.12200229437150609No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.11562904018791996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA150.004634171758.1314754
AAGAGGT150.004634171758.1314726
ATCAGGC150.004634171758.1314737
CGCGAAT150.004634171758.1314753
TAACGCT201.980001E-458.1314753
CTAACGC201.980001E-458.1314752
CGCTAAT201.980001E-458.1314756
CGAATGT150.004634171758.1314755
TACTAAC150.004634171758.1314752
CGATGGA150.004634171758.1314752
AACTATT150.004634171758.1314758
TAGACAT150.00464258758.10485550
CCTAACG201.9844923E-458.10485551
ATTAGCT150.004651013858.07826241
TGCCACT150.004651013858.07826241
TATTGTC258.548825E-658.07826241
TGCTGAT150.004651013858.07826224
AGAATAG150.004651013858.07826242
GTTATGG150.004651013858.07826241
ACCGACG258.572064E-658.05169730
Produced by FastQC (version 0.11.5)