FastQCFastQC Report
Fri 17 Jun 2016
SRR1526425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194878
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC15100.7748437483964327No Hit
ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9690.4972341670173134No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCC6990.3586859471053685No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3620.18575724299305207No Hit
ATGGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTC3590.18421781832736378No Hit
ATGGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3580.18370467677213437No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGTTCTTCC2970.1524030419031394No Hit
ATGGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2910.14932419257176283No Hit
ATGGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2840.14573220168515685No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.1344430874701095No Hit
ATGGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2160.11083857592955593No Hit
ATGGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.10775972659817937No Hit
ATGGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC2060.10570716037726166No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2050.10519401882203225No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2030.10416773571157338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG150.0080743450.53369537
ACGGTGA150.0080743450.53369537
CGGACTA150.0080743450.53369537
CGGAATA150.008082651550.52054636
ACGGAAT150.00809096850.50741235
ATAGGCT204.0029304E-450.4287233
TATGCGT150.00814100750.4287232
GCCTACG150.00814100750.4287232
ATGCGTT150.00814100750.4287233
AATAGGC204.0029304E-450.4287232
CTATTCG252.04631E-550.1422748
ACCATCG150.00832645350.14226548
AAGGCGC150.00832645350.14226548
TTCGAAC204.1223428E-450.12932647
TCGCGCA150.00834346750.11638650
TAAGCTG204.1328586E-450.10346249
ACGAGTA204.1328586E-450.10346243
CTTTAGA150.00835198350.10345544
TGATTGG150.00836050550.0905340
TACGTAC150.00836050550.0905340