##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526425_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 194878 Sequences flagged as poor quality 0 Sequence length 56 %GC 58 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.26877841521362 38.0 35.0 38.0 35.0 39.0 2 37.18555198637096 38.0 35.0 38.0 35.0 39.0 3 37.12802881802974 38.0 35.0 38.0 35.0 39.0 4 37.045931300608586 38.0 35.0 38.0 35.0 39.0 5 36.840448896232516 38.0 35.0 38.0 35.0 39.0 6 37.01393692464003 38.0 35.0 38.0 35.0 39.0 7 36.9284167530455 38.0 35.0 38.0 35.0 39.0 8 35.49555106271616 35.0 35.0 36.0 35.0 39.0 9 35.33938156179764 35.0 35.0 36.0 35.0 39.0 10 33.39034678106302 35.0 34.0 35.0 27.0 39.0 11 32.164046223791296 35.0 30.0 35.0 23.0 38.0 12 32.51942753928098 35.0 31.0 36.0 25.0 38.0 13 32.2832695327333 35.0 29.0 36.0 24.0 38.0 14 32.4686881022999 35.0 30.0 38.0 24.0 38.0 15 32.3959964695861 35.0 30.0 37.0 25.0 38.0 16 31.061068976487853 33.0 29.0 36.0 22.0 38.0 17 30.080322047640063 33.0 27.0 36.0 19.0 38.0 18 30.07291228358255 33.0 27.0 36.0 19.0 38.0 19 29.370652408173317 32.0 26.0 35.0 17.0 38.0 20 29.470622645963115 33.0 27.0 35.0 17.0 38.0 21 28.24710844733628 31.0 24.0 35.0 12.0 38.0 22 27.93035129670871 31.0 23.0 35.0 10.0 38.0 23 27.745512577099518 31.0 23.0 35.0 10.0 38.0 24 27.717479653937335 31.0 25.0 35.0 4.0 38.0 25 27.09781504325783 30.0 23.0 35.0 4.0 38.0 26 25.71212245610074 30.0 20.0 35.0 4.0 38.0 27 25.391952914130894 29.0 20.0 34.0 4.0 38.0 28 24.84138281386303 29.0 18.0 34.0 4.0 37.0 29 24.757278912960928 29.0 18.0 34.0 4.0 37.0 30 24.090590010160202 29.0 15.0 34.0 4.0 36.0 31 23.449686470509754 28.0 11.0 33.0 4.0 36.0 32 23.265345498209136 28.0 10.0 33.0 4.0 36.0 33 22.787764652757108 27.0 9.0 33.0 4.0 36.0 34 22.2804729112573 27.0 4.0 33.0 4.0 36.0 35 22.12281529982861 26.0 4.0 33.0 4.0 36.0 36 22.85325177803549 29.0 4.0 35.0 4.0 38.0 37 22.368861544145567 28.0 4.0 34.0 4.0 37.0 38 22.092770861769928 27.0 4.0 34.0 4.0 36.0 39 21.988089984503127 27.0 4.0 34.0 4.0 36.0 40 21.218244234854627 26.0 4.0 33.0 4.0 36.0 41 20.91939572450456 26.0 4.0 34.0 4.0 36.0 42 20.73735362637137 26.0 4.0 33.0 4.0 36.0 43 20.48072640318558 25.0 4.0 33.0 4.0 36.0 44 20.112496023152946 25.0 4.0 33.0 4.0 36.0 45 19.16734572399142 23.0 4.0 33.0 4.0 36.0 46 18.224745738359385 18.0 4.0 33.0 4.0 36.0 47 17.81889695091288 16.0 4.0 32.0 4.0 35.0 48 17.438951549174355 13.0 4.0 32.0 4.0 35.0 49 17.131040958958938 10.0 4.0 32.0 4.0 35.0 50 16.614882131384764 4.0 4.0 32.0 4.0 35.0 51 15.942995104629563 4.0 4.0 32.0 4.0 35.0 52 15.604213918451544 4.0 4.0 31.0 4.0 35.0 53 15.270559016410267 4.0 4.0 30.0 4.0 35.0 54 15.031517154322191 4.0 4.0 31.0 4.0 35.0 55 14.45450486971336 4.0 4.0 30.0 4.0 35.0 56 9.116139328195075 4.0 4.0 17.0 4.0 22.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5097883115586299 1 2 -0.2560161248686583 1 3 -0.878348557969268 1 4 -0.9901000767934534 1 5 -0.34196299318706025 1 6 -1.133535062053447 1 7 -1.3198830449338104 1 8 -1.0369117332052795 1 9 -1.2453716695569028 1 10 0.3244510317846405 1 11 -0.3030336825011233 1 12 -0.2755470599475558 1 13 0.32024951573383476 1 14 -0.577528998860604 1 15 -0.1536888966754546 1 16 0.006731752285514148 1 17 0.41527882085723533 1 18 1.0960943375665906 1 19 0.21137778461766388 1 20 0.43742154120936405 1 21 1.341302469104182 1 22 1.172383841561171 1 23 2.1227299123033276 1 24 1.3885801975582943 1 25 2.294116911248288 1 26 2.629755996206079 1 27 2.982096876995108 1 28 2.8829571417520334 1 29 2.905200303447515 1 30 3.533466144755959 1 31 2.953199664437701 1 32 2.9023355074660735 1 33 3.050338339407066 1 34 3.146165788266245 1 35 2.983121145289463 1 36 3.594417731964338 1 37 3.7478488193459754 1 38 4.100058357651129 1 39 3.8757239371330563 1 40 3.241757030529474 1 41 4.38929600214589 1 42 4.276112915962553 1 43 5.193255147408866 1 44 5.186521693758216 1 45 5.121988489967933 1 46 5.109436498140539 1 47 5.412605690437861 1 48 5.181428251374573 1 49 6.320754087015615 1 50 5.777518636238039 1 51 6.3737932503680454 1 52 6.696835532881387 1 53 6.009987122087741 1 54 6.972894637121161 1 55 6.016525461802981 1 56 3.546787171780922 2 1 -0.054224727743601875 2 2 0.5488369579829779 2 3 -0.05880376028140688 2 4 0.16310921993101601 2 5 0.42721451162989155 2 6 0.24694422503711166 2 7 0.36701483560568704 2 8 0.2689649527292133 2 9 0.2649245732176624 2 10 0.556447659915662 2 11 -0.26217987710613144 2 12 -0.09765086534254408 2 13 0.4765898336529091 2 14 -0.5013006751611826 2 15 -0.34444034176215865 2 16 0.26778064438570937 2 17 -0.43891795178245374 2 18 0.4632065726340251 2 19 -0.04316821730911968 2 20 0.38591022136350617 2 21 0.2389421608190183 2 22 0.66843995909489 2 23 1.075734488411225 2 24 0.6282839547837291 2 25 0.6116747147164965 2 26 0.2885951098862307 2 27 0.32792418913383514 2 28 0.13717197797553737 2 29 0.39023763485406704 2 30 -0.9388062540879716 2 31 0.6394051075976215 2 32 0.15543642268765367 2 33 0.5976047170563916 2 34 0.24846588460535912 2 35 1.0295445556940876 2 36 0.46411908071193153 2 37 0.1906173646253606 2 38 0.1725234347224216 2 39 0.27309267509066615 2 40 0.10281796501887541 2 41 -0.27941787068647983 2 42 -0.30785264280430624 2 43 0.3537199836323701 2 44 0.3911519924094691 2 45 -0.3829187836351515 2 46 -0.2126070953083996 2 47 -0.6519415157278416 2 48 0.2619918351896011 2 49 -0.21865463167417332 2 50 -0.319241961064467 2 51 0.4366545220443463 2 52 0.3951134712243558 2 53 0.395132834997181 2 54 -0.2169536287747924 2 55 -0.028091590220139295 2 56 -0.05259264517476048 3 1 -0.1758645126898415 3 2 0.4315654526066339 3 3 0.045698927890633456 3 4 0.131691209178328 3 5 0.266092199801939 3 6 -0.1310799685112798 3 7 0.08744494313257434 3 8 0.22864237208405314 3 9 0.3425455409595983 3 10 -0.06149588847143406 3 11 0.6250849078401046 3 12 0.16730209164670384 3 13 -0.661010972798703 3 14 -0.6646071267740865 3 15 -0.43046184713850266 3 16 -1.0395110222809585 3 17 -0.5425805861910575 3 18 -1.2071563305917792 3 19 -0.8017367656962158 3 20 -1.056798111969826 3 21 -1.0634771940196899 3 22 -0.651284503270702 3 23 -0.9452668556747952 3 24 -0.5111649699474548 3 25 -1.1080833497996316 3 26 -0.8276683309739816 3 27 0.6442884364456631 3 28 -0.10812372094919098 3 29 0.12326854883255933 3 30 0.06606605773998453 3 31 0.17922365598472112 3 32 -0.2123391149467544 3 33 0.8197149321101556 3 34 0.645979325465575 3 35 -0.12132910021989218 3 36 0.4090115538302115 3 37 1.2495891388189087 3 38 0.7913070368729578 3 39 1.0975214385315262 3 40 0.3980128574919952 3 41 1.1365767529694324 3 42 0.7148288088085941 3 43 1.3043248223420498 3 44 0.832180218215921 3 45 1.3564965389454962 3 46 -0.15565145014711135 3 47 0.25128429072377045 3 48 0.32180366314658926 3 49 0.5849407984333546 3 50 0.46469621097854485 3 51 0.13692333924864641 3 52 0.15889035294478404 3 53 0.631187942524063 3 54 0.7706135755262835 3 55 -0.43971793430616124 3 56 0.14213181719082968 4 1 0.18972688515962233 4 2 0.8562966354023374 4 3 0.12634408918096085 4 4 0.34136862853316785 4 5 0.7714685438879556 4 6 0.34741465514463954 4 7 0.6465847280788068 4 8 0.4759542000410377 4 9 0.3586745732176624 4 10 0.5890417459371662 4 11 0.5283107142917203 4 12 0.699560156162832 4 13 0.640064296018501 4 14 1.4052853463441934 4 15 0.5265274001733218 4 16 0.7400588701921613 4 17 0.3337634998304466 4 18 0.8089727016662849 4 19 0.3111664601102362 4 20 1.3195459740516782 4 21 1.5117916231846102 4 22 1.1390380773744582 4 23 0.990217015292945 4 24 1.295286642955773 4 25 0.7682607362218725 4 26 2.6723316690260184 4 27 2.714180909563943 4 28 2.7144569242120973 4 29 1.854451344531487 4 30 2.8725176706432123 4 31 3.6899763441567615 4 32 2.653252282902706 4 33 3.099284824583272 4 34 2.430925562024715 4 35 2.4700687492424755 4 36 3.1455706936151557 4 37 2.996900966775897 4 38 2.6138876820342496 4 39 2.807198857886366 4 40 3.5324214596425314 4 41 3.179587505657608 4 42 4.166441712034402 4 43 3.73980869330979 4 44 3.364438282732049 4 45 2.6952062163648485 4 46 3.1077894100679444 4 47 2.6760154735194703 4 48 2.725029469598205 4 49 2.7408547769279785 4 50 2.9431908346344606 4 51 2.9487512962378943 4 52 3.228782826063064 4 53 2.830112673706859 4 54 2.6630866938058517 4 55 2.554905721607817 4 56 1.2174006343951316 5 1 -0.07888688990576753 5 2 0.4157851561811796 5 3 0.2565678613402156 5 4 0.33166214785894965 5 5 0.4859701422605269 5 6 -0.024395865052980525 5 7 0.44899681758766974 5 8 0.25319918696344246 5 9 0.1992151137581999 5 10 -0.12604107882307858 5 11 0.5803595373082828 5 12 0.5491678283511447 5 13 -0.03075813932006355 5 14 0.5449831086226027 5 15 0.32042452310270875 5 16 1.2361646533947201 5 17 1.3466957869562854 5 18 1.1489610771385301 5 19 0.271555881789979 5 20 0.540499185327473 5 21 0.4616899085667683 5 22 1.079898292428222 5 23 0.5781275064292437 5 24 -0.007357937108164236 5 25 0.34579633633811824 5 26 0.5711401549312782 5 27 0.5791910810257264 5 28 1.0467440500476108 5 29 1.1924054276468574 5 30 1.6876295567228397 5 31 1.2581832607507764 5 32 1.5069285398047683 5 33 1.855431293632968 5 34 1.2345582269477013 5 35 2.1922134746130055 5 36 1.5724524140452623 5 37 0.2525824547154478 5 38 0.6118533896773748 5 39 1.6869721795951733 5 40 1.1202447668206794 5 41 2.4986226698540612 5 42 2.1547881229614596 5 43 2.2975825962449825 5 44 2.502159875292353 5 45 2.040251261409896 5 46 1.8767228596465557 5 47 0.9489593851730582 5 48 1.3582756189733836 5 49 0.9888059539114131 5 50 1.7408368677643615 5 51 2.4400329004227252 5 52 2.7846968045576865 5 53 1.992739816979162 5 54 2.4558504252792623 5 55 3.0575503016717533 5 56 1.8623003728432561 6 1 0.27594542932445876 6 2 0.7645328567613419 6 3 0.01923943343587098 6 4 -0.27130941008643106 6 5 0.4839608030784106 6 6 -0.12418361091402375 6 7 0.25793979197567296 6 8 0.015474551333049646 6 9 -0.04708458908600477 6 10 0.39245333181792574 6 11 -0.357266046390599 6 12 0.11376383797333744 6 13 -0.3258479935722214 6 14 -0.1613137641664153 6 15 -0.5015084045893836 6 16 -0.259406310239072 6 17 -0.3257253252554051 6 18 -0.9422799020605623 6 19 -0.10433204802465212 6 20 -0.5882117245352703 6 21 0.4769439060197165 6 22 -0.1766462101895776 6 23 -0.3857892899482849 6 24 -0.13431421275553745 6 25 0.40585010738665517 6 26 0.2932853367623238 6 27 0.5564091367778126 6 28 0.6497156079929916 6 29 0.5528412561455127 6 30 1.2495936150219755 6 31 0.09525698368837254 6 32 0.8645060125654886 6 33 -0.5207962428039927 6 34 0.6070500905390439 6 35 0.7162264928668627 6 36 0.3609340894379329 6 37 -0.5502476004706267 6 38 0.2781898832390013 6 39 0.5046317239562867 6 40 0.6175703646698381 6 41 1.2209093544444087 6 42 2.008219564874576 6 43 1.750589529880191 6 44 1.6241635543640953 6 45 1.3383126736074331 6 46 1.237300190904513 6 47 2.2023342259824545 6 48 1.422659374456618 6 49 1.9536566685003471 6 50 1.3158474804713123 6 51 1.7370471922014161 6 52 2.499880050630985 6 53 1.6760283411053845 6 54 2.3002256905969354 6 55 2.2148730694657246 6 56 1.1832003303924292 7 1 0.22581670872048676 7 2 0.6382131089958705 7 3 0.2504158529782643 7 4 0.45198468033617445 7 5 0.7433689775599106 7 6 0.22075868175902258 7 7 0.3053205446296303 7 8 0.14972001718593475 7 9 0.035470153328162723 7 10 0.5036737188475229 7 11 -0.25541767636948265 7 12 0.01385350850644329 7 13 -0.18596196192719816 7 14 -0.720568630962287 7 15 -0.47151216496657966 7 16 0.05993649153119662 7 17 0.2009588898381729 7 18 -0.4590425065556616 7 19 0.7275212394127983 7 20 -0.7667428633510411 7 21 -0.26359006754193715 7 22 0.052273983035956206 7 23 -0.6793011929884045 7 24 0.22376047412074485 7 25 0.7971028915120755 7 26 0.3154137102545569 7 27 1.0685146587470946 7 28 1.4899574291725912 7 29 1.1892995593476208 7 30 1.4856585249175538 7 31 0.9805365532698147 7 32 0.9993878499436377 7 33 1.3116355642018789 7 34 1.1842969159129098 7 35 1.016940964533866 7 36 0.6862645687413966 7 37 1.2925107808316199 7 38 1.3769720995474657 7 39 1.3530017450722518 7 40 1.4591427808568156 7 41 2.2796290906394887 7 42 2.3168308286505734 7 43 1.927327258033845 7 44 2.1898110623910547 7 45 1.5193314465674277 7 46 1.8164272048757617 7 47 1.551557563461845 7 48 1.5910664208249585 7 49 1.49972128913614 7 50 0.7212161420662895 7 51 1.3489473397239031 7 52 1.0863542631212226 7 53 1.2910524942973662 7 54 1.0128460949821125 7 55 1.0905202882328986 7 56 0.33636338613279015 8 1 0.11404450302563163 8 2 0.7355356759317004 8 3 0.276704481476834 8 4 0.4062983224951182 8 5 0.775486031776417 8 6 0.42906418840707516 8 7 0.6069415755324314 8 8 0.8010162347804908 8 9 0.7652679798110711 8 10 0.47826542548342843 8 11 0.2509268078755511 8 12 0.6924704716537917 8 13 1.0883686798067558 8 14 0.2864740501135401 8 15 0.5731420758202574 8 16 0.570379087609151 8 17 0.9303013705618675 8 18 -0.033931705754252306 8 19 1.194068504302603 8 20 -0.22712778229949748 8 21 2.044345091221949 8 22 1.4535246660545944 8 23 1.413589341891079 8 24 1.548270218519992 8 25 1.0560428465846279 8 26 2.052102435893559 8 27 1.24224424407889 8 28 2.0215584248619862 8 29 2.2505283857698153 8 30 1.9394561085493898 8 31 1.428839631407147 8 32 0.06380409668032527 8 33 0.5803199185215924 8 34 0.8577836501731255 8 35 1.6754923578918905 8 36 1.1339908755837236 8 37 2.0035180056509994 8 38 1.9188031966271808 8 39 2.1974859901089836 8 40 1.5268678734437842 8 41 1.9437964150278049 8 42 1.6300479982213325 8 43 2.3373509360133227 8 44 2.155174428306907 8 45 2.1311036522622864 8 46 2.756200139123834 8 47 2.362710498924173 8 48 2.156909417607185 8 49 2.3259309910364507 8 50 1.7193340462615403 8 51 1.4572589176487405 8 52 0.9890924089532938 8 53 0.7880472122865587 8 54 1.3864117558405908 8 55 0.988735332856784 8 56 0.24061935116223587 9 1 0.1737780655524901 9 2 0.5743839784671536 9 3 0.20254400508171955 9 4 0.1817602906945197 9 5 0.8456560851866897 9 6 0.21615977438533918 9 7 0.4517448169837124 9 8 0.40630201045733116 9 9 0.47878630506123443 9 10 0.22014644948735196 9 11 0.5361499646070911 9 12 0.01934750523473383 9 13 -0.7270589093638513 9 14 0.415947928190775 9 15 0.7617049096344886 9 16 0.1504331117972555 9 17 0.6787205491486432 9 18 0.05078804376996615 9 19 0.6502966337150902 9 20 0.9124756310469344 9 21 -0.15235206636906184 9 22 0.8061552756684449 9 23 1.355267775189624 9 24 1.6532490385826115 9 25 1.521940077844988 9 26 1.0017003240389322 9 27 1.4191427645528307 9 28 1.6324000971561716 9 29 0.980990660924828 9 30 1.2220701425600744 9 31 0.34264649493468013 9 32 0.7454911247360698 9 33 0.9607914023450306 9 34 1.4907261266910226 9 35 0.8341884104426924 9 36 1.4908881682352089 9 37 2.4611888264503143 9 38 2.9343658462680438 9 39 2.0435477495785648 9 40 1.6622046037525813 9 41 1.2139480511012302 9 42 1.562579154216177 9 43 1.361692516220824 9 44 2.4781224300258593 9 45 2.1803086372214615 9 46 2.296442020147836 9 47 2.4439616500077257 9 48 2.8594080363069203 9 49 2.0486579847131665 9 50 2.0378925826971717 9 51 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120 56 0.6228134282715665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 211.0 5 355.0 6 407.0 7 180.0 8 620.0 9 777.0 10 839.0 11 2725.0 12 3410.0 13 3978.0 14 5476.0 15 5057.0 16 5834.0 17 6089.0 18 6254.0 19 6182.0 20 5766.0 21 6263.0 22 7129.0 23 7902.0 24 7884.0 25 7881.0 26 8333.0 27 8903.0 28 9639.0 29 10541.0 30 10914.0 31 10927.0 32 10767.0 33 9921.0 34 8812.0 35 6380.0 36 4345.0 37 2746.0 38 1162.0 39 269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.58029348813575 1.0927531322339077 0.22173290423910197 0.10522047539124053 8 98.44004967210255 1.24180256365521 0.2021777727603937 0.1159699914818502 9 96.37157606297274 2.42100185757243 0.3309763031229795 0.8764457763318588 10 62.26305688687282 22.90150760988926 6.625170619567114 8.21026488367081 11 45.590061474358315 22.168228327466416 14.990917394472438 17.25079280370283 12 37.12476523773848 13.371442646168372 24.21822884060797 25.285563275485174 13 26.184074138691898 18.15443508246185 26.858855283818595 28.802635495027655 14 27.46846745143115 18.13596198647359 29.145927195476144 25.249643366619118 15 33.71853159412556 18.738903313868164 25.292747257258384 22.249817834747894 16 38.172600293516965 19.31721384661173 22.59362267675161 19.916563183119695 17 32.710208438099734 21.609417173821573 24.01502478473712 21.66534960334158 18 34.61601617422182 22.07688913063558 22.258541241186794 21.048553453955808 19 31.547429673949857 28.30848017734172 19.024723160130954 21.119366988577468 20 33.737004690113814 25.080819794948635 20.811482055439814 20.370693459497737 21 29.686778394687956 26.026026539681236 23.027227290920475 21.259967774710333 22 28.811872043021786 20.48409774320344 29.25522634674001 21.448803867034762 23 32.504605469090755 19.461506488708263 24.992687695315507 23.04120034688547 24 34.62696983225316 19.553359298429264 22.685078280145937 23.134592589171632 25 30.834573730944925 19.160806857260177 20.852538110147307 29.15208130164759 26 24.5447778433738 19.551323185913397 27.65830745142329 28.24559151928951 27 24.084337039985616 18.632219625568197 24.177816584914865 33.10562674953132 28 25.6690753894273 24.545860746036393 22.48997221494641 27.295091649589903 29 32.199096789440965 21.26192592440364 22.068547413416635 24.470429872738762 30 31.522772959327945 18.679533384402617 26.200599464286633 23.597094191982805 31 36.728072926574704 18.5427024581356 22.567957346385807 22.161267268903888 32 40.22397234553676 16.632801609061776 22.405979454626824 20.737246590774642 33 39.15786332130967 17.924863254416252 22.49831480042606 20.41895862384802 34 30.996701164627837 21.15834924425277 29.551080484378133 18.293869106741255 35 28.667311770397973 28.34922072841819 25.825080809536555 17.15838669164728 36 27.931068216104332 30.953398448159568 22.555233471148846 18.56029986458725 37 32.57179681734557 26.60094169984097 21.173694768680683 19.653566714132772 38 30.95571792202974 27.21539206694354 21.305790574729226 20.523099436297496 39 34.641603932540846 22.47504804893004 20.545877066876898 22.337470951652215 40 28.5916387424553 20.988336371005634 21.830967735273312 28.589057151265756 41 25.36132122655859 24.72246714206898 22.172207011410684 27.744004619961743 42 23.723446501049505 20.152552075543706 23.013806014409415 33.11019540899737 43 24.476527590014552 18.57256375063209 25.609126840796275 31.341781818557084 44 26.24645768057441 18.347795115857718 23.868909662466642 31.53683754110123 45 29.676756622824225 20.127604220437323 22.88668414858252 27.308955008155934 46 25.772636905837704 23.502620639797577 22.99553329374403 27.729209160620695 47 25.29015736648631 19.98781545578456 25.964437652306806 28.75758952542233 48 27.0082536991767 19.394384176878273 27.89254169487671 25.70482042906832 49 29.9919389010035 18.327115263380907 25.039530389309743 26.641415446305842 50 26.135236244884464 17.523562481914762 26.02155760406763 30.31964366913315 51 23.955486440826828 18.67699254490879 28.950345889367068 28.41717512489732 52 22.99156519402121 18.605672820491616 28.211635070600156 30.19112691488702 53 22.97552957231027 17.983748410507708 32.486637926578375 26.554084090603645 54 24.105517868960952 18.18952680344143 29.437251819986766 28.267703507610854 55 24.410421869587022 18.408384651578842 24.80442190061065 32.37677157822348 56 25.473932423907087 20.69427351046116 21.761073936560795 32.070720129070956 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 3.5 7 7.0 8 5.0 9 3.0 10 11.0 11 19.0 12 52.0 13 106.0 14 127.0 15 148.5 16 170.0 17 199.5 18 229.0 19 361.5 20 494.0 21 652.0 22 742.5 23 675.0 24 736.5 25 798.0 26 1002.5 27 1207.0 28 1459.5 29 1958.0 30 2204.0 31 2418.5 32 2633.0 33 2759.0 34 2885.0 35 2635.0 36 2385.0 37 2470.5 38 2649.0 39 2742.0 40 2702.5 41 2663.0 42 2836.0 43 3009.0 44 3174.0 45 3339.0 46 3588.0 47 4677.0 48 5517.0 49 5443.5 50 5370.0 51 5466.0 52 5562.0 53 5970.5 54 6869.5 55 7360.0 56 8324.5 57 9289.0 58 9890.0 59 10491.0 60 11898.5 61 13306.0 62 14397.0 63 16355.5 64 17223.0 65 16103.0 66 14983.0 67 13684.0 68 12385.0 69 10903.0 70 9421.0 71 8304.0 72 6432.5 73 5678.0 74 4982.0 75 4286.0 76 3820.5 77 3355.0 78 2866.0 79 2004.0 80 1631.0 81 1371.0 82 1111.0 83 922.0 84 733.0 85 586.5 86 440.0 87 377.0 88 234.5 89 155.0 90 112.5 91 70.0 92 54.5 93 39.0 94 42.0 95 45.0 96 69.5 97 47.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.025143936206241852 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 5.131415552294256E-4 24 5.131415552294256E-4 25 0.024630794651012426 26 0.042590749084042326 27 0.09390490460698489 28 0.08672092283377292 29 0.12264083169983271 30 0.1903755169901169 31 0.19550693254241114 32 0.24579480495489484 33 0.27658329826866035 34 0.2909512618150843 35 0.3048060838062788 36 0.3371340017857326 37 1.2623282258643869 38 0.41256581040445817 39 0.41307895195968763 40 0.6152567247200813 41 0.4808136372499718 42 0.5013392994591488 43 0.5521403134268619 44 0.58959964695861 45 0.5921653547347572 46 0.6275721220455874 47 0.6106384507230165 48 0.7127536202136722 49 0.6958199488911011 50 0.6927410995597245 51 0.6768337113476124 52 0.7153193279898192 53 0.7286610084257844 54 0.7543180861872556 55 0.7579100770738616 56 0.7676597666232207 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 194878.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.48261257919707 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01111988480035 41.72238501668073 2 10.15282775803874 10.859995071330076 3 4.146353325390352 6.652734255549528 4 2.308479373034464 4.938540318202798 5 1.368042603799062 3.6583246285410613 6 0.9103598426899506 2.9213053664547246 7 0.6155669692916281 2.3045490814622247 8 0.4406899794790869 1.8855401152011446 9 0.32876871424559634 1.5825068794942176 >10 1.5978759087960568 15.318410054948314 >50 0.07794516628256126 2.818690064534806 >100 0.03897258314128063 3.6385365869080317 >500 0.0019985940072451604 0.8914628417982663 >1k 9.992970036225802E-4 0.807019718894114 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1510 0.7748437483964327 No Hit ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 969 0.4972341670173134 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCC 699 0.3586859471053685 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 362 0.18575724299305207 No Hit ATGGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTC 359 0.18421781832736378 No Hit ATGGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 358 0.18370467677213437 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGTTCTTCC 297 0.1524030419031394 No Hit ATGGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 291 0.14932419257176283 No Hit ATGGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 284 0.14573220168515685 No Hit ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 262 0.1344430874701095 No Hit ATGGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 216 0.11083857592955593 No Hit ATGGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 210 0.10775972659817937 No Hit ATGGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC 206 0.10570716037726166 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 205 0.10519401882203225 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 203 0.10416773571157338 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0010262831104588513 0.0 0.0 0.0 0.0 12 0.0015394246656882768 0.0 0.0 0.0 0.0 13 0.0015394246656882768 0.0 0.0 0.0 0.0 14 0.003591990886605979 0.0 0.0 0.0 0.0 15 0.003591990886605979 0.0 0.0 0.0 0.0 16 0.003591990886605979 0.0 0.0 0.0 0.0 17 0.005131415552294256 0.0 0.0 0.0 0.0 18 0.0056445571075236815 0.0 0.0 0.0 0.0 19 0.006670840217982533 0.0 0.0 0.0 0.0 20 0.007697123328441384 0.0 0.0 0.0 0.0 21 0.00923654799412966 0.0 0.0 0.0 0.0 22 0.013341680435965066 0.0 0.0 0.0 0.0 23 0.01744681287780047 0.0 0.0 0.0 0.0 24 0.018986237543488747 0.0 0.0 0.0 0.0 25 0.020012520653947596 0.0 0.0 0.0 0.0 26 0.020525662209177023 0.0 0.0 0.0 0.0 27 0.02103880376440645 0.0 0.0 0.0 0.0 28 0.023091369985324153 0.0 0.0 0.0 0.0 29 0.02463079465101243 0.0 0.0 0.0 0.0 30 0.025143936206241856 0.0 0.0 0.0 0.0 31 0.027709643982388982 0.0 0.0 0.0 0.0 32 0.02822278553761841 0.0 0.0 0.0 0.0 33 0.029249068648077258 0.0 0.0 0.0 0.0 34 0.03027535175853611 0.0 0.0 0.0 0.0 35 0.030788493313765534 0.0 0.0 0.0 0.0 36 0.030788493313765534 0.0 0.0 0.0 0.0 37 0.03335420108991266 0.0 0.0 0.0 0.0 38 0.03386734264514209 0.0 0.0 0.0 0.0 39 0.034380484200371514 0.0 0.0 0.0 0.0 40 0.03489362575560094 0.0 0.0 0.0 0.0 41 0.035919908866059794 0.0 0.0 0.0 0.0 42 0.03745933353174807 0.0 0.0 0.0 0.0 43 0.038998758197436346 0.0 0.0 0.0 0.0 44 0.042590749084042326 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTACG 15 0.00807434 50.533695 37 ACGGTGA 15 0.00807434 50.533695 37 CGGACTA 15 0.00807434 50.533695 37 CGGAATA 15 0.0080826515 50.520546 36 ACGGAAT 15 0.008090968 50.507412 35 ATAGGCT 20 4.0029304E-4 50.42872 33 TATGCGT 15 0.008141007 50.42872 32 GCCTACG 15 0.008141007 50.42872 32 ATGCGTT 15 0.008141007 50.42872 33 AATAGGC 20 4.0029304E-4 50.42872 32 CTATTCG 25 2.04631E-5 50.14227 48 ACCATCG 15 0.008326453 50.142265 48 AAGGCGC 15 0.008326453 50.142265 48 TTCGAAC 20 4.1223428E-4 50.129326 47 TCGCGCA 15 0.008343467 50.116386 50 TAAGCTG 20 4.1328586E-4 50.103462 49 ACGAGTA 20 4.1328586E-4 50.103462 43 CTTTAGA 15 0.008351983 50.103455 44 TGATTGG 15 0.008360505 50.09053 40 TACGTAC 15 0.008360505 50.09053 40 >>END_MODULE