FastQCFastQC Report
Fri 17 Jun 2016
SRR1526424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63715
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.7941615004316095No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG2600.40806717413481913No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2460.3860943262967904No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.3641214784587617No Hit
CCGACGGGGCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACT2300.3609825001961861No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.27779957623793455No Hit
CCGACGGGGAAGACAAGCAATCACACAGCAGAATATTATAGAACCATCTCACAGAACCTTGATC1450.22757592403672605No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1370.21502001098642393No Hit
CCGACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1350.21188103272384837No Hit
CCGACGGGACCACCGCCGCCACTGCGTCTGCCGCCCTAGGCTTTCAGACATCATTAGTTCCGAC1260.19775563054225848No Hit
CCGACGGGGACCTACAACAATCTGTCATCGGATTCCAAACTGAGGACCAAACAGCTGTTTGAAT1230.1930471631483952No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1150.18049125009809305No Hit
CCGACGGGGAGCTTTCCAGGTTTATTCAGGGGGCTTCATCTCCAGATGACCACCGCTCGCTCGC1140.17892176096680532No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1110.17421329357294202No Hit
CCGACGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA1080.16950482617907872No Hit
CCGACGGGGAGCCATTTCTTTTTTCATCTCCTTATTTTTGCTTTCTGTGCAGCTGTTGTGTATG1050.16479635878521542No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1030.1616573805226399No Hit
CCGACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1020.1600878913913521No Hit
CCGACGGGGTCCGACGGGGCGTCATTTGGCTGCTACTTGCTTAGTGACTCCAGGCTGCATCATA1020.1600878913913521No Hit
CCGACGGGGGACGTCAGCGGCGCCCGCGGTCCTGTTGGTGCCATCTAACCCATCCCTGGCCTGG990.1553794239974888No Hit
CCGACGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC880.13811504355332338No Hit
CCGACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG860.13497606529074788No Hit
CCGACGGGGACCTGACATCCTCACAGTACACACAAAGGGCTAGCCCCGAATGTCACATGAATGT850.13340657615946008No Hit
CCGACGGGGGAACTCGGTCCTGTCCTTGGTTTGGGTATATGGGTGTTAGGCGTTGTATTCTTTC830.13026759789688455No Hit
CCGACGGGGATTTTAGAATTTGTTTATTTTTACTTGCATAGGGAGTGAGTTTTAATCCATGAGT830.13026759789688455No Hit
CCGACGGGGATTGCATGGTGGCTTCTAGTACTCCTTTGTGCTTTTGTCTATGTTACTGTAAGAA810.12712861963430905No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC780.12242015224044574No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT780.12242015224044574No Hit
CCGACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.12085066310915796No Hit
CCGACGGGGTAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.1192811739778702No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC740.11614219571529467No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGTCTGCCCTTCCAGAGGTCCT740.11614219571529467No Hit
CCGACGGGGATTTCTAAACTTATATAATGACCTGATTTTCTTTCTTGCAATTTTTCCCCCTTTT710.11143372832143138No Hit
CCGACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG700.10986423919014361No Hit
CCGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10515577179628031No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA670.10515577179628031No Hit
CCGACGGGGATCACAGTCCTGGTCAGGGTTCTCTGGCTTGCTGGATGAACCTGCAGTGTCTGCC660.10358628266499254No Hit
CCGACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.10201679353370478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCA150.00462569958.13002850
CAGAACC150.00462569958.13002852
GACAGGC150.00462569958.13002858
CAACACT150.00462569958.13002858
TCTACTG150.00462569958.13002858
CTTGATC150.00462569958.13002858
GGTGAGC150.00462569958.13002848
AACTCTG150.00462569958.13002849
TTTGAAT201.9738945E-458.13002858
GCGCTCA150.00462569958.13002847
CCTTGAT150.00462569958.13002857
CGCCCGT150.00462569958.13002850
CGAATGT258.462906E-658.13002447
ACATGAA258.462906E-658.13002455
ATCCTAT201.981607E-458.08425538
GATCGCG150.004640163458.08425544
GAACTAA201.981607E-458.08425540
TTCGTCC150.004640163458.08425538
ATAGAAC150.004640163458.08425538
AGAACCA150.004640163458.08425540
Produced by FastQC (version 0.11.5)