FastQCFastQC Report
Fri 17 Jun 2016
SRR1526423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61195
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.6209657651768935No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.3398970504126154No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1870.30558052128441865No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG1700.27780047389492607No Hit
CCGACGGGGCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACT1690.27616635346025No Hit
CCGACGGGGAAGACAAGCAATCACACAGCAGAATATTATAGAACCATCTCACAGAACCTTGATC1410.23041098128932103No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.21733801781191275No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1100.17975324781436391No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1050.17158264564098374No Hit
CCGACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1030.16831440477163168No Hit
CCGACGGGACCACCGCCGCCACTGCGTCTGCCGCCCTAGGCTTTCAGACATCATTAGTTCCGAC990.16177792303292754No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG970.15850968216357544No Hit
CCGACGGGGACCTACAACAATCTGTCATCGGATTCCAAACTGAGGACCAAACAGCTGTTTGAAT930.1519732004248713No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC910.14870495955551924No Hit
CCGACGGGGTCCGACGGGGCGTCATTTGGCTGCTACTTGCTTAGTGACTCCAGGCTGCATCATA840.137266116512787No Hit
CCGACGGGGAGCTTTCCAGGTTTATTCAGGGGGCTTCATCTCCAGATGACCACCGCTCGCTCGC820.13399787564343493No Hit
CCGACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG780.12746139390473077No Hit
CCGACGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC750.12255903260070267No Hit
CCGACGGGGATTTTAGAATTTGTTTATTTTTACTTGCATAGGGAGTGAGTTTTAATCCATGAGT730.1192907917313506No Hit
CCGACGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA730.1192907917313506No Hit
CCGACGGGGATTTCTAAACTTATATAATGACCTGATTTTCTTTCTTGCAATTTTTCCCCCTTTT720.11765667129667458No Hit
CCGACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.11602255086199853No Hit
CCGACGGGGGAACTCGGTCCTGTCCTTGGTTTGGGTATATGGGTGTTAGGCGTTGTATTCTTTC700.11438843042732248No Hit
CCGACGGGGACCTGACATCCTCACAGTACACACAAAGGGCTAGCCCCGAATGTCACATGAATGT690.11275430999264646No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG680.11112018955797041No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC640.10458370781926628No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC620.10131546694991421No Hit
CCGACGGGGAGCCATTTCTTTTTTCATCTCCTTATTTTTGCTTTCTGTGCAGCTGTTGTGTATG620.10131546694991421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTG150.004569617658.30603852
TACTGTA150.004569617658.30603854
ACACTCG150.004569617658.30603858
CGAACTG258.307863E-658.30603454
CTGACGG258.307863E-658.30603458
TATATGG150.004584618458.2578536
ACAGAAG150.004584618458.2578551
TAGATGC150.004584618458.2578553
ACCCGTT201.9518482E-458.2578536
GTTGATG258.348758E-658.25784736
CTATGTT150.00459965558.20974348
CCCGTTG201.959848E-458.20974337
CTCTACG406.7848305E-1058.20974337
CCTGATT150.00459965558.20974331
GCATCCT150.00459965558.20974350
TCTATGT150.00459965558.20974347
AACATCG258.389819E-658.2097449
TATGGGT150.00461472958.16171638
ACCTGAT150.00461472958.16171630
CCAGCGA150.00461472958.16171638
Produced by FastQC (version 0.11.5)