SRR1526422_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58291
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	571	0.9795680293698856	No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	243	0.4168739599595135	No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	237	0.4065807757629823	No Hit
CCGACGGGGCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCA	185	0.31737317939304527	No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	170	0.29164021890171726	No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	156	0.26762278910981113	No Hit
CCGACGGGGAAGACAAGCAATCACACAGCAGAATATTATAGAACCATCTCACAGAA	142	0.24360535931790497	No Hit
CCGACGGGGGACGTCAGCGGCGCCCGCGGTCCTGTTGGTGCCATCTAACCCATCCC	135	0.23159664442195194	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	127	0.217872398826577	No Hit
CCGACGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	113	0.19385496903467087	No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	110	0.1887083769364053	No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	102	0.17498413134103033	No Hit
CCGACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	101	0.17326860064160848	No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	100	0.1715530699421866	No Hit
CCGACGGGACCACCGCCGCCACTGCGTCTGCCGCCCTAGGCTTTCAGACATCATTA	91	0.15611329364738982	No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	90	0.15439776294796795	No Hit
CCGACGGGGAGTAGCTGTTCACCTTGAAGACAGGCCTTAGTGTCACGCAGGTTTAT	87	0.14925117084970235	No Hit
CCGACGGGAAGCACACCCTGAGCAAGAAGGAGCTGAAGGAGTTGATCCAGAAGGAG	86	0.14753564015028048	No Hit
CCGACGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCAT	85	0.14582010945085863	No Hit
CCGACGGGGAGCTTTCCAGGTTTATTCAGGGGGCTTCATCTCCAGATGACCACCGC	85	0.14582010945085863	No Hit
CCGACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	83	0.1423890480520149	No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	83	0.1423890480520149	No Hit
CCGACGGGGACCTACAACAATCTGTCATCGGATTCCAAACTGAGGACCAAACAGCT	82	0.14067351735259304	No Hit
CCGACGGGGATTGCATGGTGGCTTCTAGTACTCCTTTGTGCTTTTGTCTATGTTAC	81	0.13895798665317116	No Hit
CCGACGGGGACCTGACATCCTCACAGTACACACAAAGGGCTAGCCCCGAATGTCAC	80	0.1372424559537493	No Hit
CCGACGGGGAGCCATTTCTTTTTTCATCTCCTTATTTTTGCTTTCTGTGCAGCTGT	77	0.1320958638554837	No Hit
CCGACGGGGGAACTCGGTCCTGTCCTTGGTTTGGGTATATGGGTGTTAGGCGTTGT	76	0.13038033315606182	No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA	73	0.12523374105779622	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	73	0.12523374105779622	No Hit
CCGACGGGGATTTTAGAATTTGTTTATTTTTACTTGCATAGGGAGTGAGTTTTAAT	71	0.12180267965895249	No Hit
CCGACGGGGCAGCTGGGACATCCAGTCCTCGCTGCTGTTGCTGCTAGTTCGAAGGA	70	0.12008714895953064	No Hit
CCGACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	69	0.11837161826010877	No Hit
CCGACGGGGTCCGACGGGGCGTCATTTGGCTGCTACTTGCTTAGTGACTCCAGGCT	68	0.1166560875606869	No Hit
CCGACGGGGGTTAATGTTCAGTTTGTTCAGCCGGGATCGATGTTTGCTGGCATCGC	68	0.1166560875606869	No Hit
CCGACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	67	0.11494055686126503	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGTCTGCCCTTCCA	67	0.11494055686126503	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	66	0.11322502616184316	No Hit
CCGACGGGGGCAACTTTTGGGAACCGTCGAAAGCGAAGGTAATGTACAAGAAATTG	66	0.11322502616184316	No Hit
CCGACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.10979396476299944	No Hit
CCGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG	62	0.1063629033641557	No Hit
CCGACGGGGATTTCTAAACTTATATAATGACCTGATTTTCTTTCTTGCAATTTTTC	61	0.10464737266473384	No Hit
CCGACGGGGGGTCGTTGTTCTGCCTTCTTTCAGTATCTGATCACCCACCAAAGGTT	59	0.1012163112658901	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATTA	15	0.00795436	50.67951	37
GTGCATT	15	0.00795436	50.67951	36
TATATGG	15	0.00795436	50.67951	36
ATGCTTG	15	0.00795436	50.67951	36
ATATGGG	15	0.00795436	50.67951	37
GCTGAAC	15	0.00795436	50.67951	37
TTAGTGT	15	0.00795436	50.67951	37
CGACCCA	15	0.00795436	50.67951	36
ATTATAG	20	3.8997232E-4	50.635174	35
TATTATA	20	3.8997232E-4	50.635174	34
ATTTTTG	20	3.8997232E-4	50.635174	34
CGCGTGG	20	3.8997232E-4	50.635174	35
TATGCTT	15	0.007981964	50.635166	35
GTATATG	15	0.007981964	50.635166	35
GGCGCGT	20	3.9335692E-4	50.54673	33
GTAGGAT	45	1.1823431E-10	50.54673	33
ATATTAT	20	3.9335692E-4	50.54673	33
GCGAAGG	15	0.008037386	50.546722	33
ACTGGTT	15	0.008037386	50.546722	32
TTGGGTA	15	0.008065204	50.502617	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content