FastQCFastQC Report
Fri 17 Jun 2016
SRR1526421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343751
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.6277799919127508No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12630.3674171129683987No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12360.3595625903633735No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10800.314180904201006No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10500.30545365686208914No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9780.2845082632486887No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7340.21352665155883183No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.17105404784276992No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5340.1553450026327196No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5140.14952683774010841No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.14836320476158615No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5030.1463268470491722No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4770.13876323268877763No Hit
GATCTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4490.13061780183912192No Hit
GATCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4220.12276327923409677No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4070.11839965556463836No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4020.11694511434148555No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3860.11229058242739658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAAT258.5276515E-658.147145
CGTCGTT150.004640368758.13859640
AGATAGT150.00465112358.10461436
GTTCGTT202.0223946E-457.91843826
GCGCTAA202.0223946E-457.91843824
ACTAGAC150.00471332257.91000423
GCGTAAT202.0340715E-457.8510319
CTAGATT150.00473510157.84261711
TAGCGTT150.00473510157.84261711
GAAGTTA303.8250437E-757.8426179
GAACATA150.00473510157.8426179
GGCTATA150.00473510157.84261711
GATTACT202.0355349E-457.8426139
TGGGACC3500.053.7116
TTGGGAC18400.052.6556435
TTGGGGC34550.052.485275
TTGGGAT21850.052.151015
CGTTAAA950.051.8822131
ATCGGCT451.9899744E-951.6938748
CTATCAG451.9954314E-951.67875341
Produced by FastQC (version 0.11.5)