FastQCFastQC Report
Fri 17 Jun 2016
SRR1526420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336620
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.5653258867565801No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9730.28904996732220306No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9590.2848909749866318No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8480.2519161071831739No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8090.2403303428197968No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7910.23498306695977658No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5900.1752718198562177No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5020.14912958231834114No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4570.13576139266829065No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.13516725090606618No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.12120491949379121No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.11734299803933218No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3420.10159824134038382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG150.004618022658.2092258
TTACTAT351.5945261E-858.19179556
CGCTATC150.004623502558.1917954
TATGGCA201.9978282E-458.061440
GCACGAT201.9978282E-458.061441
CGCACGA201.9978282E-458.061440
CATCCGA202.0245297E-457.90569324
TAATGTG202.027514E-457.88844315
ACACCGG150.00472296957.87981813
GTTAGTC150.004736908757.8367516
CGTAAAG202.0379864E-457.82815621
TTGGGGC34250.052.8924065
TGGGATT5050.052.635996
TGGGGCT15650.052.6158836
TTGGGAT19550.052.0214275
TTGGGAC18100.050.921185
CTTACTA404.570211E-850.91782455
AAGCAGT17850.050.02316710
TACATAC351.0610947E-649.89362339
CAATCCC351.071001E-649.8264649
Produced by FastQC (version 0.11.5)