FastQCFastQC Report
Fri 17 Jun 2016
SRR1526419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307962
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT24350.7906819672557004No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA14340.4656418649054104No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA12030.390632610516882No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.37861814119923887No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA10530.34192530247238295No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9840.3195199407719134No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6070.19710223988673928No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5850.18995850137354608No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.18313947824731622No Hit
GATCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.17989232437768296No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA5160.16755313967307656No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA5000.16235769348166332No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.14352420103779037No Hit
GATCTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3950.12826257785051404No Hit
GATCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA3880.12598957014177073No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3790.1230671316591008No Hit
GATCTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG3730.12111883933732083No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3560.11559867775894428No Hit
GATCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3550.11527396237198095No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3290.10683136231093447No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA3190.10358420844130121No Hit
GATCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA3150.1022853468934479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGACG150.00800391450.65287837
CTATCAT203.926261E-450.63612436
CGTCAGT203.926261E-450.63612436
CGATGCA150.00825282550.26202448
CGATACA204.0759257E-450.25377346
AACGTGA204.0792287E-450.2455344
ACGATAC204.0792287E-450.2455345
AACGATA204.0792287E-450.2455344
TCCGTCA204.0792287E-450.2455340
AGGGTTA150.00826354250.24552543
CTAAGCG150.00826354250.24552543
GTTACGG150.00826354250.24552544
TTACGGA150.00826354250.24552545
CACGTCT150.00826354250.24552540
CGTTGTA150.00826890650.2372839
TCACGTC150.00826890650.2372839
CGTCGTC252.0341704E-550.2043338
TTACCGG150.00833346250.13855441
AAAGGTA150.00846370149.94225330
GACGTAT204.2165018E-449.90968328
Produced by FastQC (version 0.11.5)