FastQCFastQC Report
Fri 17 Jun 2016
SRR1526418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206931
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC7480.36147314805418235No Hit
TAGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC7240.34987507913265775No Hit
TAGTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT6740.32571243554614826No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6100.29478425175541606No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5660.2735211253992877No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5620.2715881139123669No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4530.21891355089377618No Hit
TAGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC4420.21359776930474408No Hit
TAGTCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4170.20151644751148934No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3870.18701886135958362No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCCGAAAGTCC3740.18073657402709117No Hit
TAGTCGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA3620.17493753956632888No Hit
TAGTCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT3570.1725212752076779No Hit
TAGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG3380.1633394706448043No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3300.1594734476709628No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT3260.15754043618404204No Hit
TAGTCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3180.1536744132102005No Hit
TAGTCGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC3170.1531911603384703No Hit
TAGTCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGCG2950.14255959716040614No Hit
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2890.139660079930025No Hit
TAGTCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2780.13434429834099287No Hit
TAGTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2590.12516249377811928No Hit
TAGTCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2590.12516249377811928No Hit
TAGTCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2500.12081321793254757No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.11984671218908718No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2460.11888020644562682No Hit
TAGTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC2440.11791370070216643No Hit
TAGTCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2390.11549743634351547No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2380.11501418347178527No Hit
TAGTCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCATTCAGC2250.10873189613929281No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2230.10776539039583242No Hit
TAGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGC2130.10293286167853052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGTA150.004622361658.18740556
TATGGCA201.9794873E-458.15913448
CTATAAT150.004631267458.15913440
CGGTCTA150.004631267458.15913441
ACATCGG201.9794873E-458.15913452
TAGTGTG150.004631267458.15913440
TATAATG150.004631267458.15913441
TCGACAA150.004631267458.15913446
ACAAGTT201.9794873E-458.15913449
TACACCG150.004631267458.15913449
CAAAGTA201.9794873E-458.15913443
CCCTATA150.00466253858.06039438
TCCTACG150.00466253858.06039435
TATGTCG150.00466253858.06039438
ACGACAG201.996191E-458.0603938
TCGTTTT150.00467150158.03223833
CACCTAA150.00467150158.03223832
ACGAAGC150.00467150158.03223834
ACGGGTA303.7635073E-757.96198729
TACGGGA202.015418E-457.9479628
Produced by FastQC (version 0.11.5)