FastQCFastQC Report
Fri 17 Jun 2016
SRR1526417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198927
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6830.3433420299909012No Hit
TAGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6580.33077460575990186No Hit
TAGTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4900.2463215149275865No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4860.24431072705062662No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4810.24179724220442675No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4330.21766778768090808No Hit
TAGTCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3900.19605181800358926No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3670.18448978771106989No Hit
TAGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3560.17896012104943018No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCCGAAAGTCC3410.1714196665108306No Hit
TAGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG3270.164381908941471No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3190.16036033318755122No Hit
TAGTCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3100.15583606046439144No Hit
TAGTCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT2960.14879830289503185No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT2900.14578212107959201No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2710.13623087866403255No Hit
TAGTCGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC2530.12718233321771302No Hit
TAGTCGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2490.12517154534075314No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2410.12114996958683337No Hit
TAGTCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGCG2320.1166256968636736No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2260.11360951504823377No Hit
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2260.11360951504823377No Hit
TAGTCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2210.11109603020203392No Hit
TAGTCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2190.11009063626355396No Hit
TAGTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC2130.10707445444811414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAT150.004620164658.1935239
AGTCTAA150.00462480158.1787835
ATTAGTA150.00462944158.1640557
AATTAGT150.00462944158.1640556
AATCCGT150.00463408458.1493350
CCGTATC150.00463873158.13461753
TCCGTAT150.00463873158.13461752
AATGCGG150.00464803558.1052151
ATAGGGG150.00466668458.0464943
CAGCTAC150.00466668458.0464940
ATACCAA150.00466668458.0464943
CAAAGTA258.60227E-658.0464943
GACTTCG201.9983597E-458.04648643
CCGATAT150.004671355258.0318331
ATCACGA150.0046760358.01717430
TACGGGA258.641007E-658.00252528
CGTAGCA202.0083666E-457.98788527
ATACGGG258.653951E-657.98788527
TGATACG258.666913E-657.9732525
GATACGG258.666913E-657.9732526
Produced by FastQC (version 0.11.5)