FastQCFastQC Report
Fri 17 Jun 2016
SRR1526416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191791
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14310.7461246878112112No Hit
TAGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT7320.3816654587545818No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC6980.3639378281566914No Hit
TAGTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG5360.279470882366743No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.21377436897456084No Hit
TAGTCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA3980.2075175581753054No Hit
TAGTCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT3700.19291833297704272No Hit
TAGTCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3440.17936190957865591No Hit
TAGTCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA3150.1642412834804553No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3070.16007007628095166No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3010.15694167088132394No Hit
TAGTCGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT2910.15172766188194442No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC2840.14807785558237874No Hit
TAGTCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2820.14703505378250284No Hit
TAGTCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTT2770.14442804928281305No Hit
TAGTCGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCC2750.14338524748293716No Hit
TAGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG2730.14234244568306126No Hit
TAGTCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2540.13243582858424013No Hit
TAGTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA2490.12982882408455038No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.1293074231846124No Hit
TAGTCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG2370.12357201328529493No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2270.1183580042859154No Hit
TAGTCGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2250.1173152024860395No Hit
TAGTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC2250.1173152024860395No Hit
TAGTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2180.11366539618647381No Hit
TAGTCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2170.11314399528653586No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2140.11157979258672202No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2090.10897278808703224No Hit
TAGTCGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC2060.10740858538721837No Hit
TAGTCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTT2050.10688718448728042No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2040.10636578358734247No Hit
TAGTCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG2030.10584438268740452No Hit
TAGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2020.10532298178746657No Hit
TAGTCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1960.10219457638783885No Hit
TAGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG1930.10063037368802498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATA203.957361E-450.54538334
GTTATAA203.957361E-450.54538335
AGGTATC251.9791376E-550.4252933
GTAGGAT150.0081429950.4252933
TTACCCT150.008168519550.38538431
GCACCCA150.00833159450.1341239
ACGCACC150.00835756250.09467750
GCTATTG150.00835756250.09467750
ACGTATA150.00835756250.09467744
AAGATAG150.00835756250.09467748
GCCCGTT150.00835756250.09467746
AGATAGT150.00836623250.0815449
AAACGTA150.00836623250.0815442
GGCTATT150.00836623250.0815449
CAAACGT150.0083922850.0421841
AACGGTT451.364242E-1049.93751529
GGTTTAG204.2009188E-449.9375129
TACGGGA355.3214535E-849.92446528
TAGTTCG150.00847078849.92446528
ACCCCTA150.00847078849.92446528
Produced by FastQC (version 0.11.5)