FastQCFastQC Report
Fri 17 Jun 2016
SRR1526415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42274
Sequences flagged as poor quality0
Sequence length64
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4200.9935184747125894No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2640.624497326962199No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2290.5417041207361498No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.5322420400246014No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.5298765198467144No Hit
TCGTAAGGGACCATAAAATTTTCCATTTGTTCTGCATTTTGATTCTGAAAAGAAAGCTGGCTTT2110.49912475753418173No Hit
TCGTAAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG1950.4612764346879879No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.45181435397643943No Hit
TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT1800.4257936320196811No Hit
TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1730.4092349907744713No Hit
TCGTAAGGGCCACCCATGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA1530.36192458721672893No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1090.2578416993896958No Hit
TCGTAAGGGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTC1000.2365520177887117No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA940.22235889672138903No Hit
TCGTAAGGGGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGCTT930.2199933765435019No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC930.2199933765435019No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG910.21526233618772767No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.19160713440885652No Hit
TCGTAAGGGGATGACAGCATGGAGTGTGTGGACAGTGAGCGGAGGCCGCACTTCCCCCAGTTCT790.18687609405308225No Hit
TCGTAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA780.18451057387519515No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA710.16795193262998534No Hit
TCGTAAGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAA690.1632208922742111No Hit
TCGTAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.15848985191843687No Hit
TCGTAAGGGGCTCACTTCATGTGTAACAGGTGTCCTCATGTTGTGCTGTATTCTGTGCTTTTTC640.1513932913847755No Hit
TCGTAAGGGCACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAG630.1490277712068884No Hit
TCGTAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG620.1466622510290013No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAGAAGGT620.1466622510290013No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG590.13956569049533993No Hit
TCGTAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTGGC580.1372001703174528No Hit
TCGTAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.1324691299616786No Hit
TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.1324691299616786No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC550.13010360978379146No Hit
TCGTAAGGGGGGCAGTGGCTAACAGCTGTTGTCGGTCCAGCAGTGGCTGGGGTAAACTTTCTAC550.13010360978379146No Hit
TCGTAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAG550.13010360978379146No Hit
TCGTAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG510.12064152907224299No Hit
TCGTAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG500.11827600889435586No Hit
TCGTAAGGGAGACTTAAATACACACAACTGGTCAATAAAGGCAAAAGTAGAGCTCTCTCTATAG500.11827600889435586No Hit
TCGTAAGGGGTGCCTACAGGGTCCGTCCCGTCCCCCAGCCTGTCCTACGTGTGTGCTCTCTAGA490.11591048871646874No Hit
TCGTAAGGGATTACTACCTATTAGCATCCCAGGACAAATTCTCGACACCCTACTTTATCTTAAG490.11591048871646874No Hit
TCGTAAGGGGTAACATAGTTATTTATCAGCACTTTATGGAAGCAAGCATTGTTGACGGAGTCAG470.11117944836069452No Hit
TCGTAAGGGCCACAATTATTGACACAGTTGTTAAATGTCTTTAGTTTTCTGCCATTTAGTGAGA460.1088139281828074No Hit
TCGTAAGGGGAGCCAGACCCAGGGGCCTCCTGATAACCCCGGCTTTGTGGAGTCTGTGGATGAA460.1088139281828074No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTGAGAAGC440.10408288782703316No Hit
TCGTAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC440.10408288782703316No Hit
TCGTAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.10171736764914605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTTC150.004528539758.40718550
GTTTGAT150.004528539758.40718548
CTTTATC150.004528539758.40718553
GCCTTAA150.004528539758.40718558
ATCTTAA150.004528539758.40718557
CGCCTTA150.004528539758.40718557
GCGATGA150.004528539758.40718549
TTCCGCC150.004528539758.40718554
CGCCATA150.004528539758.40718554
ATTGGCG150.004528539758.40718555
GTATTCT258.1785365E-658.40718548
GATTGGC150.004528539758.40718554
ACTCATT150.004528539758.40718556
TCTAATC150.004528539758.40718548
TATCTTA150.004528539758.40718556
TAACCAC150.004528539758.40718551
AGAAAGC201.9203436E-458.40718551
CTAATCG150.004528539758.40718549
TAATCGC150.004528539758.40718550
ACCCTAC150.004528539758.40718547
Produced by FastQC (version 0.11.5)