FastQCFastQC Report
Fri 17 Jun 2016
SRR1526414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41100
Sequences flagged as poor quality0
Sequence length64
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3370.8199513381995134No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2850.6934306569343066No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.5669099756690997No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.5085158150851582No Hit
TCGTAAGGGACCATAAAATTTTCCATTTGTTCTGCATTTTGATTCTGAAAAGAAAGCTGGCTTT1820.4428223844282238No Hit
TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1360.3309002433090025No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1330.3236009732360097No Hit
TCGTAAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG1260.3065693430656934No Hit
TCGTAAGGGCCACCCATGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA1250.30413625304136255No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.2944038929440389No Hit
TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT1150.2798053527980535No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1050.25547445255474455No Hit
TCGTAAGGGGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGCTT830.20194647201946472No Hit
TCGTAAGGGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTC770.18734793187347934No Hit
TCGTAAGGGGATGACAGCATGGAGTGTGTGGACAGTGAGCGGAGGCCGCACTTCCCCCAGTTCT740.18004866180048662No Hit
TCGTAAGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAA720.1751824817518248No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA700.17031630170316303No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.17031630170316303No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA690.1678832116788321No Hit
TCGTAAGGGCACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAG680.16545012165450124No Hit
TCGTAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA660.16058394160583941No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG630.1532846715328467No Hit
TCGTAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.15085158150851583No Hit
TCGTAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTGGC590.1435523114355231No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC580.1411192214111922No Hit
TCGTAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG540.1313868613138686No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAGAAGGT520.1265206812652068No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC510.12408759124087593No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG510.12408759124087593No Hit
TCGTAAGGGATTACTACCTATTAGCATCCCAGGACAAATTCTCGACACCCTACTTTATCTTAAG490.1192214111922141No Hit
TCGTAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG470.11435523114355231No Hit
TCGTAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA450.10948905109489052No Hit
TCGTAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10705596107055962No Hit
TCGTAAGGGCCACAATTATTGACACAGTTGTTAAATGTCTTTAGTTTTCTGCCATTTAGTGAGA440.10705596107055962No Hit
TCGTAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC430.10462287104622871No Hit
TCGTAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG430.10462287104622871No Hit
TCGTAAGGGGCTCACTTCATGTGTAACAGGTGTCCTCATGTTGTGCTGTATTCTGTGCTTTTTC430.10462287104622871No Hit
TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.10218978102189782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATA406.4028427E-1058.49012433
CAGTTAC201.9062322E-458.49012434
AAATATA406.4028427E-1058.49012435
TCAGTAC452.7284841E-1158.49012436
CTCAGTA406.4028427E-1058.49012435
GTTACCT201.9062322E-458.49012436
AGTTACC201.9062322E-458.49012435
ATTAGAT150.00450219658.4901235
GGATTAG150.00450219658.4901233
CCCATTA150.00450219658.4901233
TTAGATG150.00450219658.4901236
ACCACAA150.00452428358.41800338
GTATCTA150.00452428358.41800339
GATGTAG150.00452428358.41800339
TCTACGT303.493642E-758.41800338
AGTACGT452.7284841E-1158.41800338
TTGATTC150.00452428358.41800339
CTACGTA303.493642E-758.41800339
ACCTTAG201.9179228E-458.41800339
CATGATT406.693881E-1058.2027132
Produced by FastQC (version 0.11.5)