##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41100
Sequences flagged as poor quality	0
Sequence length	64
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.384476885644766	39.0	38.0	40.0	35.0	40.0
2	38.11187347931873	39.0	38.0	40.0	35.0	40.0
3	38.05990267639903	39.0	38.0	40.0	35.0	40.0
4	37.89377128953771	39.0	38.0	40.0	34.0	40.0
5	38.19510948905109	39.0	38.0	40.0	35.0	40.0
6	38.31588807785888	39.0	38.0	40.0	35.0	40.0
7	38.26323600973236	39.0	38.0	40.0	35.0	40.0
8	38.27639902676399	39.0	38.0	40.0	36.0	40.0
9	38.460267639902675	39.0	38.0	40.0	36.0	40.0
10	37.63279805352798	39.0	38.0	40.0	33.0	40.0
11	37.738394160583944	39.0	38.0	40.0	34.0	40.0
12	37.601508515815084	39.0	38.0	40.0	33.0	40.0
13	37.52771289537713	39.0	38.0	40.0	33.0	40.0
14	37.47846715328467	39.0	37.0	40.0	33.0	40.0
15	37.33936739659367	39.0	38.0	40.0	33.0	40.0
16	37.1007299270073	39.0	37.0	40.0	33.0	40.0
17	36.81452554744526	39.0	36.0	40.0	32.0	40.0
18	37.101849148418495	39.0	36.0	40.0	33.0	40.0
19	37.05793187347932	39.0	36.0	40.0	32.0	40.0
20	36.864939172749395	39.0	36.0	40.0	31.0	40.0
21	37.00262773722628	39.0	36.0	40.0	32.0	40.0
22	36.970097323600974	39.0	36.0	40.0	32.0	40.0
23	36.80136253041363	39.0	36.0	40.0	31.0	40.0
24	36.60802919708029	38.0	36.0	40.0	31.0	40.0
25	36.614233576642334	38.0	36.0	40.0	31.0	40.0
26	36.897128953771286	39.0	36.0	40.0	32.0	40.0
27	36.34155717761557	39.0	36.0	40.0	30.0	40.0
28	36.30484184914842	39.0	36.0	40.0	30.0	40.0
29	36.23863746958637	39.0	36.0	40.0	30.0	40.0
30	36.28226277372263	39.0	36.0	40.0	30.0	40.0
31	35.93265206812652	39.0	36.0	40.0	30.0	40.0
32	36.12620437956205	39.0	35.0	40.0	30.0	40.0
33	36.076836982968366	39.0	35.0	40.0	30.0	40.0
34	35.44097323600973	39.0	35.0	40.0	27.0	40.0
35	35.67416058394161	39.0	35.0	40.0	29.0	40.0
36	35.008856447688565	38.0	35.0	40.0	27.0	40.0
37	34.679537712895375	38.0	35.0	40.0	25.0	40.0
38	34.6930900243309	38.0	34.0	40.0	26.0	40.0
39	34.23464720194647	38.0	34.0	40.0	23.0	40.0
40	34.417250608272504	38.0	34.0	40.0	25.0	40.0
41	33.93729927007299	38.0	34.0	40.0	21.0	40.0
42	33.55484184914842	38.0	33.0	40.0	17.0	40.0
43	33.649294403892945	38.0	33.0	40.0	20.0	40.0
44	33.42508515815085	38.0	33.0	39.0	18.0	40.0
45	33.34827250608272	38.0	33.0	39.0	18.0	40.0
46	33.108223844282236	38.0	33.0	39.0	12.0	40.0
47	32.90116788321168	38.0	33.0	39.0	10.0	40.0
48	32.77160583941606	38.0	32.0	39.0	10.0	40.0
49	32.60759124087591	37.0	32.0	39.0	6.0	40.0
50	32.16	37.0	31.0	39.0	4.0	40.0
51	31.784136253041364	37.0	31.0	39.0	4.0	40.0
52	31.67749391727494	37.0	31.0	39.0	4.0	40.0
53	31.37822384428224	36.0	30.0	39.0	4.0	40.0
54	31.034768856447688	36.0	30.0	39.0	4.0	40.0
55	30.80756690997567	36.0	30.0	39.0	4.0	40.0
56	30.20498783454988	36.0	29.0	39.0	4.0	40.0
57	29.84411192214112	36.0	28.0	39.0	4.0	40.0
58	29.57021897810219	35.0	28.0	39.0	4.0	40.0
59	29.23793187347932	35.0	26.0	39.0	4.0	40.0
60	28.92199513381995	35.0	26.0	39.0	4.0	40.0
61	28.579367396593675	35.0	24.0	39.0	4.0	40.0
62	28.351265206812652	35.0	23.0	39.0	4.0	40.0
63	27.999586374695863	35.0	21.0	39.0	4.0	40.0
64	27.863576642335765	35.0	19.0	39.0	4.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	-0.4616308964264988
2	2	-0.21085417015056862
2	3	-0.26946506838529416
2	4	-0.33705434056398786
2	5	-0.406439196198761
2	6	0.051817967957219935
2	7	0.08436645294318623
2	8	0.289339818405864
2	9	-0.22148861055433855
2	10	0.03063957952349483
2	11	0.0034167789087220513
2	12	-0.4397425884907875
2	13	-1.2622269976409086
2	14	-0.7358092549181663
2	15	0.13921071728778145
2	16	-0.6918171045551063
2	17	-0.38666029250205014
2	18	-0.23295747128420885
2	19	-0.6930644301611153
2	20	-0.11514146262925351
2	21	-1.1422121706109607
2	22	-1.385567749674621
2	23	-1.7156487446039819
2	24	-1.7381103340627106
2	25	-0.8323857561014236
2	26	-0.8613595926775801
2	27	-0.6164735346834149
2	28	-0.07590476962223391
2	29	0.20394190984845295
2	30	-1.0200120393769652
2	31	-1.0582306073186274
2	32	-0.6001390039272749
2	33	-1.510585282117205
2	34	-0.48721428763150954
2	35	-0.5092096298575584
2	36	-3.226162530833445
2	37	-3.52031245550301
2	38	-3.4525885812635586
2	39	-7.42409487827101
2	40	-4.707115233951924
2	41	-4.498162485122389
2	42	-5.009895152115199
2	43	-5.567876390582054
2	44	-5.325537964972458
2	45	-3.993620436825225
2	46	-3.0269221465648783
2	47	-3.4449068317764713
2	48	-4.744292687768667
2	49	-4.602711283139488
2	50	-4.390569444529259
2	51	-4.961130414189711
2	52	-5.224961102579147
2	53	-4.590661328666403
2	54	-4.371948835917145
2	55	-5.225473737900213
2	56	-4.773055360503971
2	57	-4.400299147897108
2	58	-6.525763577790521
2	59	-4.7586121559015275
2	60	-4.932165707058406
2	61	-4.471334608013883
2	62	-5.178890701210186
2	63	-4.283496375182796
2	64	-4.324090825356926
3	1	0.16117612111736435
3	2	0.45648726034875864
3	3	0.11245126089945501
3	4	0.13865416146030185
3	5	0.3688644475259366
3	6	0.10310001923927103
3	7	-0.3866187022524983
3	8	0.26707261192812837
3	9	-0.3550918494207309
3	10	-0.4626127821499182
3	11	-0.352858524734998
3	12	-0.49507322276878796
3	13	-1.6542647844155383
3	14	0.43625552240976617
3	15	-0.08953422198347027
3	16	-0.6580789129221785
3	17	0.1464031352982218
3	18	-1.0028630043476383
3	19	0.25970210155278295
3	20	0.44963586530597155
3	21	-0.49106507209544503
3	22	-0.056957763169897646
3	23	-1.2163235084366377
3	24	-0.3480968387860557
3	25	-1.051009237882802
3	26	-0.12519225124707845
3	27	0.2728651967605771
3	28	0.5057416541294515
3	29	1.1088002094435936
3	30	0.2998260173031966
3	31	-0.45971508775047454
3	32	-0.14129959771945266
3	33	-0.4734732713209837
3	34	0.6956467110189593
3	35	-0.11447278775229819
3	36	-5.132370358093905
3	37	-4.15324093053675
3	38	-4.372966449009848
3	39	-20.880235229148205
3	40	-4.694969484964069
3	41	-4.202615926417934
3	42	-4.000448458457978
3	43	-4.887039683429556
3	44	-4.195983309102012
3	45	-4.230462542088382
3	46	0.574292428333905
3	47	0.5092092276027458
3	48	0.5127923324742483
3	49	0.6003926304907381
3	50	0.6478920939322812
3	51	2.3209208678615703
3	52	1.4140402469485167
3	53	1.0584614783511412
3	54	1.482976940061267
3	55	1.5498299058244847
3	56	2.2363913331532537
3	57	2.084856047784406
3	58	1.7394186084442964
3	59	1.4917252260148004
3	60	0.41061431993214725
3	61	0.5036991301777505
3	62	0.5593009317182904
3	63	0.7084064588253014
3	64	0.9255717927267462
4	1	0.06217862738302671
4	2	0.16826670897031448
4	3	0.6700953711751438
4	4	0.39554639203674213
4	5	0.3701175803580199
4	6	0.6745285906678404
4	7	0.5056118741886095
4	8	0.6831127121787617
4	9	0.5935297044639825
4	10	0.48851503739895463
4	11	1.0418783173702622
4	12	-0.4324165811647802
4	13	0.23044699503308408
4	14	-0.3218898409987574
4	15	0.37913745721452585
4	16	0.40708399434598874
4	17	0.785500879659125
4	18	-0.07178531011204825
4	19	0.935140698044016
4	20	1.0185581710703815
4	21	0.018959990561199902
4	22	0.8653480012411308
4	23	-0.14238867134390176
4	24	-0.17767077362314865
4	25	0.046735123019452374
4	26	1.307138575820595
4	27	1.3981784799685997
4	28	1.3929596992422333
4	29	1.5812312871378253
4	30	1.6356656163006917
4	31	0.9600844109963873
4	32	0.9401540363657617
4	33	0.6505868790549556
4	34	0.5878772874600671
4	35	-4.509209629857558
4	36	0.5430682383973249
4	37	-0.15950659469714878
4	38	0.09319896452398524
4	39	0.7663813122051764
4	40	1.2122986854619953
4	41	1.4615444745845707
4	42	0.46446382224377913
4	43	0.012709690004022889
4	44	0.775194635760144
4	45	-0.08885853206331973
4	46	-1.3254569450996776
4	47	-0.15369804056767578
4	48	-0.5849520284280096
4	49	-0.89208857251678
4	50	-0.661631715591529
4	51	-0.46479341785271444
4	52	-0.34034571796376056
4	53	-0.6382803762854508
4	54	0.5218240578557491
4	55	0.12251161008513733
4	56	0.5822559948073867
4	57	0.2352319876340303
4	58	0.7782657262387822
4	59	0.08571018842081557
4	60	0.8040980292053312
4	61	2.334526197846923
4	62	1.341255818936336
4	63	1.5003864086999847
4	64	1.9744439731778733
5	1	0.09670243690683833
5	2	1.3051714708750737
5	3	0.042714418794197684
5	4	0.7110225825129319
5	5	0.8820223422627791
5	6	0.5281000192392682
5	7	0.5318023503790812
5	8	0.8604936645597121
5	9	0.7054344663687431
5	10	1.6242293231132408
5	11	-1.2081216826297378
5	12	-1.237178485926684
5	13	-1.1397911002050094
5	14	-1.3242707933797035
5	15	-0.5184815904045266
5	16	-18.42386838660639
5	17	-2.418070548912304
5	18	-2.6015472148739534
5	19	-2.823192635289317
5	20	-2.0016799241677106
5	21	-0.8608019142007066
5	22	-0.9727472368541115
5	23	-0.5400077189629542
5	24	-1.0336231545755297
5	25	-1.399693448409117
5	26	1.3392814329634462
5	27	1.613654670444788
5	28	1.1965311278136639
5	29	1.4943265252330633
5	30	1.7511418067768787
5	31	0.7981796490916295
5	32	0.46396356017528717
5	33	0.9291583076263876
5	34	1.2140677636505401
5	35	-5.009209629857558
5	36	0.23473490506398775
5	37	0.23096959577904386
5	38	0.9967703930954173
5	39	1.2592384550623237
5	40	0.9563463045096157
5	41	-2.8289317158916205
5	42	-19.193869511089556
5	43	-3.1646712623769275
5	44	-1.7986148880493822
5	45	-1.8686204368252248
5	46	-1.873075992718725
5	47	-1.2263170881867254
5	48	-1.2885234569994353
5	49	-1.695660001088207
5	50	-1.0021079060677174
5	51	2.095920867861569
5	52	2.89298761536957
5	53	1.4676720046669303
5	54	1.615871676903371
5	55	3.2129878005613257
5	56	2.9179702805216685
5	57	2.98880341620546
5	58	3.247313345286404
5	59	3.478567331277958
5	60	4.442193267300567
5	61	3.6023833407040655
5	62	2.519827247507763
5	63	3.199195932509511
5	64	3.013729687463588
6	1	0.22170243690683833
6	2	0.8051714708750737
6	3	0.2927144187941977
6	4	0.18602258251293335
6	5	0.30702234226277625
6	6	0.3781000192392696
6	7	0.5068023503790826
6	8	0.6104936645597121
6	9	0.4054344663687388
6	10	1.0992293231132422
6	11	0.6918783173702607
6	12	0.43782151407331327
6	13	0.4852088997949906
6	14	-0.5742707933797035
6	15	1.1565184095954777
6	16	-0.898868386606388
6	17	1.131929451087693
6	18	0.7484527851260481
6	19	0.9768073647106803
6	20	0.148320075832288
6	21	0.1141980857992948
6	22	0.15225276314588854
6	23	0.8099922810370472
6	24	0.6163768454244689
6	25	0.6253065515908816
6	26	0.7392814329634518
6	27	1.0886546704447895
6	28	0.8965311278136667
6	29	0.9193265252330676
6	30	0.6011418067768801
6	31	-0.726820350908369
6	32	-0.06103643982471141
6	33	0.12915830762638336
6	34	-0.6109322363494556
6	35	0.06579037014244449
6	36	-6.49026509493601
6	37	-5.89403040422096
6	38	-6.228229606904584
6	39	-23.115761544937676
6	40	-7.493653695490384
6	41	-8.90393171589162
6	42	-9.068869511089556
6	43	-8.489671262376927
6	44	-8.723614888049383
6	45	-9.193620436825224
6	46	-3.9480759927187243
6	47	-4.826317088186727
6	48	-4.138523456999437
6	49	-4.495660001088208
6	50	-4.07710790606772
6	51	-2.7040791321384283
6	52	-2.4570123846304277
6	53	-2.8323279953330704
6	54	-2.159128323096631
6	55	-3.3620121994386736
6	56	-2.582029719478328
6	57	-2.911196583794542
6	58	-2.152686654713598
6	59	-2.021432668722042
6	60	-1.3328067326994315
6	61	-0.9976166592959359
6	62	-0.9051727524922377
6	63	-0.22580406749048976
6	64	-0.18627031253641135
7	1	-0.8206052554008565
7	2	-0.6211105804069774
7	3	-1.3463881453083673
7	4	-1.0037210072306593
7	5	-1.1500289397885055
7	6	-0.25587433973508666
7	7	-0.27460790603117147
7	8	-0.01835248928644262
7	9	-0.29841168747741165
7	10	-0.6873091384252206
7	11	-0.22735245186050435
7	12	-0.4910246397728386
7	13	-0.467355202769113
7	14	-0.581963101072013
7	15	0.11356969164675945
7	16	-0.7943812071192085
7	17	-0.23281413865589684
7	18	-1.1816754200021578
7	19	-0.9494746865713708
7	20	-0.4997568472446332
7	21	-1.731955760354552
7	22	-1.87274723685411
7	23	-1.6900077189629528
7	24	-2.507341103293477
7	25	-2.2682831919988615
7	26	-1.9895647208827043
7	27	-2.129294047503926
7	28	-1.9476996414171026
7	29	-1.3858016798951382
7	30	-1.3789863983513229
7	31	-6.314640863728886
7	32	-6.446292850081122
7	33	-7.433662205194128
7	34	-24.0769578773751
7	35	-7.970748091396018
7	36	-8.96975227442319
7	37	-7.340825276015831
7	38	-7.555152683827661
7	39	-7.936915391091524
7	40	-7.809679336516027
7	41	-1.7802137671736702
7	42	-2.8304079726280165
7	43	-2.106337929043594
7	44	-1.6332302726647647
7	45	-2.634646077850867
7	46	-2.334614454257185
7	47	-2.1884965753662122
7	48	-2.9237798672558455
7	49	-1.9616856421138493
7	50	-2.3649284188882334
7	51	-1.1662586193179152
7	52	-1.250602128220173
7	53	-1.6419433799484544
7	54	0.08958962562131845
7	55	-0.32803784046431517
7	56	-0.1576707451193542
7	57	0.0868803392823807
7	58	-1.4231994752264185
7	59	-1.6560480533374253
7	60	-0.9065246814173804
7	61	-1.31748845416773
7	62	-1.8199163422358282
7	63	-2.027086118772541
7	64	-1.8112703125364114
8	1	-0.7624439045565765
8	2	-0.3015358461980995
8	3	-0.5383831421814165
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118	19	0.3518073647106803
118	20	-0.5766799241677134
118	21	0.01419808579929338
118	22	0.3022527631458871
118	23	0.2099922810370458
118	24	-0.1086231545755254
118	25	-0.6246934484091184
118	26	0.5642814329634476
118	27	1.0886546704447895
118	28	1.121531127813661
118	29	0.9943265252330633
118	30	0.7011418067768815
118	31	0.7231796490916267
118	32	1.1389635601752843
118	33	0.7291583076263848
118	34	1.3640677636505387
118	35	0.36579037014244165
118	36	-0.040265094936010826
118	37	1.0059695957790424
118	38	1.5467703930954144
118	39	1.8092384550623208
118	40	0.35634630450961424
118	41	1.4210682841083795
118	42	0.6061304889104449
118	43	1.110328737623071
118	44	-0.048614888049378635
118	45	-0.543620436825222
118	46	0.1769240072812721
118	47	0.5736829118132718
118	48	0.21147654300056473
118	49	1.179339998911793
118	50	0.5728920939322784
118	51	2.1709208678615717
118	52	2.217987615369573
118	53	1.8676720046669288
118	54	2.290871676903368
118	55	0.9379878005613271
118	56	0.5929702805216728
118	57	1.2888034162054574
118	58	0.12231334528640403
118	59	1.6785673312779572
118	60	1.39219326730057
118	61	0.27738334070406623
118	62	0.9448272475077637
118	63	0.37419593250951166
118	64	0.9887296874635894
119	1	-0.006346343580965197
119	2	0.039927568436048944
119	3	-0.757895337303367
119	4	0.09821770446415456
119	5	-0.20944107237136933
119	6	0.2494414826539071
119	7	0.20131454550103456
119	8	0.5501278109011736
119	9	0.31884910051508086
119	10	0.1400829816498259
119	11	0.3467563661507498
119	12	0.1262361482196539
119	13	0.3181357290632789
119	14	0.45743652369346677
119	15	0.5388354827662099
119	16	-0.09581960611858875
119	17	0.8447343291364717
119	18	0.04784302902848481
119	19	0.12619760861311846
119	20	0.27697861241765054
119	21	0.2361493053114927
119	22	0.3467649582678405
119	23	0.2124313054272946
119	24	0.8560109917659346
119	25	-0.036888570360339656
119	26	0.9691594817439366
119	27	1.0520693045911287
119	28	0.08982381074049073
119	29	0.6729850618184301
119	30	-0.7104435590767793
119	31	0.003057697872115739
119	32	-0.32871936665397783
119	33	-0.23791486310532406
119	34	0.27138483682127656
119	35	0.6371318335570777
119	36	0.1365641733566747
119	37	-0.1031767456843724
119	38	-0.06359546056312126
119	39	-0.3590542278645046
119	40	-1.1930439393928225
119	41	-1.436248789062354
119	42	-1.271308535479804
119	43	-0.6835737014013148
119	44	-0.7608100100006041
119	45	-1.091181412434981
119	46	-1.5560028219870183
119	47	-0.8232683076989211
119	48	0.09623264056153857
119	49	-0.5889526840150374
119	50	-0.1691810767994255
119	51	-0.5327376687237972
119	52	-0.40945140902067223
119	53	0.5719402973498546
119	54	-0.4688844206576057
119	55	-1.199207321389892
119	56	-1.766176060941742
119	57	-1.3483917057457617
119	58	-0.7734183620306716
119	59	-0.4177741321366746
119	60	-0.44561161074821243
119	61	0.4773833407040655
119	62	0.842997979215081
119	63	0.6339520300704855
119	64	0.5058028581952954
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	23.0
5	3.0
6	11.0
7	18.0
8	25.0
9	23.0
10	37.0
11	38.0
12	57.0
13	73.0
14	87.0
15	137.0
16	155.0
17	189.0
18	207.0
19	239.0
20	253.0
21	355.0
22	476.0
23	450.0
24	569.0
25	547.0
26	483.0
27	594.0
28	736.0
29	858.0
30	1092.0
31	1239.0
32	1492.0
33	1791.0
34	2365.0
35	2830.0
36	3865.0
37	5258.0
38	7294.0
39	7204.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.40145985401459	0.4939172749391728	0.0656934306569343	0.038929440389294405
8	99.54951664353374	0.25567974285923006	0.11688216816421945	0.07792144544281296
9	99.51094890510949	0.2968369829683698	0.0656934306569343	0.1265206812652068
10	47.323600973236005	34.24087591240876	5.931873479318734	12.503649635036496
11	27.96593673965937	28.6301703163017	20.289537712895378	23.114355231143552
12	38.19221411192214	17.958637469586375	25.00243309002433	18.846715328467152
13	25.35523114355231	17.08272506082725	29.92214111922141	27.639902676399025
14	20.552311435523116	22.65450121654501	33.20681265206812	23.586374695863746
15	24.542775734846455	23.83167328251711	30.333877213062856	21.29167376957358
16	25.783683800623052	26.742601246105917	24.15547118380062	23.318243769470403
17	28.816119679360668	23.69709816533879	21.628535925736422	25.858246229564113
18	27.01703163017032	24.323600973236008	25.4330900243309	23.226277372262775
19	26.240875912408757	24.330900243309003	25.644768856447687	23.78345498783455
20	21.676483473689334	26.590566129581855	27.697999318502653	24.03495107822616
21	23.172749391727493	22.656934306569344	30.717761557177614	23.452554744525546
22	22.734793187347933	22.54257907542579	32.52068126520681	22.201946472019465
23	22.854014598540147	24.474452554744524	31.22141119221411	21.450121654501217
24	22.158689992457237	27.97148349108251	27.316966349546217	22.552860166914037
25	25.55087531348153	25.752964378758737	28.521341092254875	20.174819215504858
26	22.827594597882957	22.83246136999635	28.918359897797785	25.42158413432291
27	20.071195201638464	20.129711805724874	34.32974106402692	25.46935192860974
28	18.939596988377478	20.740235374381715	33.11079164737701	27.209375989863794
29	20.924568283118592	22.149694327398496	34.21341062425409	22.712326765228827
30	22.256610210795664	21.030827342512488	36.02778116242232	20.684781284269526
31	20.828863968795712	20.30472940029254	40.529010238907844	18.337396392003903
32	21.805024244048635	18.435223313272093	39.82115445530079	19.938597987378476
33	22.3051045780313	19.192140802496223	39.405684754521964	19.097069864950512
34	16.925481004004297	20.895595272975875	45.119152260962984	17.05977146205684
35	17.43519715184472	25.157892170011458	41.40554512424102	16.0013655539028
36	20.16156587104017	23.475862742226777	40.511055791477524	15.851515595255528
37	18.9312567132116	24.948735475051265	40.110829020603454	16.009178791133678
38	20.90576171875	22.69775390625	41.43798828125	14.958496093750002
39	19.230109949310677	20.72140460856576	39.49849401278252	20.549991429341038
40	20.591970303799943	20.70430790270587	41.22789879847611	17.475822995018074
41	16.79329636234823	21.52053355483351	41.36760071336086	20.318569369457407
42	15.924594733367565	19.91491234504511	44.47541504682266	19.685077874764666
43	16.856570003423148	19.15497090322265	46.41058242456844	17.57787666878576
44	18.74831993353046	19.212629212384837	43.8576769873659	18.1813738667188
45	17.543945431875414	20.6879690976212	40.451800601422875	21.31628486908051
46	16.918938745567917	21.166401760606433	41.031910991563755	20.88274850226189
47	15.42900457246253	21.57615473017581	43.80516908330684	19.18967161405482
48	17.394918942709733	25.043401716507326	40.72181333594151	16.83986600484143
49	19.7485876397075	23.527109980679402	38.89310083396513	17.831201545647975
50	15.582698944997919	24.054536998506844	40.068048858100994	20.294715198394243
51	15.167453580252953	27.024977371137805	40.619419233309685	17.188149815299557
52	17.03855940497162	26.50469759248385	40.14728909767078	16.309453904873752
53	19.43472409152086	25.065459439618255	38.91349565642971	16.586320812431175
54	16.78496255323315	23.76523569435606	39.25057516275882	20.199226589651964
55	19.90893735771462	25.18665393748011	37.27203740422511	17.632371300580157
56	16.95433957644755	23.545109560533724	37.66434080058759	21.836210062431142
57	16.933509596553076	27.42851547199373	37.372698785742266	18.265276145710928
58	17.34763864393494	29.64187732706251	35.85390946502058	17.156574563981973
59	21.327977662935655	26.157877978887555	35.77359229959098	16.74055205858581
60	21.57333660090675	25.737042029163092	35.7456194093861	16.94400196054405
61	17.940744368266408	25.41380999020568	36.02105778648384	20.624387855044073
62	16.731155409707597	25.718763775285304	36.38879365234853	21.161287162658567
63	16.565033183944358	26.899811427032056	35.33171699360811	21.20343839541547
64	18.60522122809168	27.211668096580468	34.50667974016424	19.676430935163623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	5.0
8	8.0
9	36.0
10	88.0
11	112.0
12	128.0
13	274.5
14	405.0
15	551.5
16	643.0
17	588.0
18	642.0
19	696.0
20	818.5
21	1100.5
22	1260.0
23	1330.5
24	1469.0
25	1537.0
26	1503.5
27	1258.5
28	1047.0
29	1065.0
30	1117.5
31	1152.0
32	975.5
33	799.0
34	874.0
35	928.0
36	907.0
37	903.5
38	896.5
39	893.0
40	918.0
41	1029.5
42	1116.0
43	1132.5
44	1149.0
45	1176.0
46	1222.0
47	1241.0
48	1285.5
49	1251.0
50	1172.0
51	1137.0
52	1234.5
53	1367.0
54	1451.0
55	1560.5
56	1586.0
57	1772.0
58	1958.0
59	2088.5
60	2068.5
61	1918.0
62	1630.5
63	1097.0
64	851.0
65	745.0
66	523.0
67	407.0
68	372.5
69	338.0
70	281.5
71	196.5
72	168.0
73	143.5
74	82.5
75	46.0
76	37.0
77	23.5
78	19.0
79	14.5
80	8.5
81	7.0
82	6.0
83	5.0
84	2.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.08029197080291971
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.09002433090024331
16	0.029197080291970805
17	0.1386861313868613
18	0.0
19	0.0
20	0.0340632603406326
21	0.0
22	0.0
23	0.0
24	0.0024330900243309003
25	0.0705596107055961
26	0.012165450121654502
27	0.20924574209245742
28	0.1435523114355231
29	0.10462287104622871
30	0.15815085158150852
31	0.19464720194647203
32	0.1435523114355231
33	0.1897810218978102
34	0.3503649635036496
35	0.2214111922141119
36	0.3065693430656934
37	0.3309002433090025
38	0.34063260340632606
39	0.6399026763990268
40	0.36982968369829683
41	0.40632603406326034
42	0.48905109489051096
43	0.49148418491484186
44	0.43552311435523117
45	0.4793187347931873
46	0.4987834549878345
47	0.4939172749391728
48	0.4939172749391728
49	0.51338199513382
50	0.6009732360097324
51	0.5425790754257908
52	0.5547445255474452
53	0.5717761557177615
54	0.5888077858880778
55	0.6058394160583942
56	0.6204379562043796
57	0.6131386861313868
58	0.6715328467153284
59	0.6593673965936739
60	0.7177615571776156
61	0.6326034063260341
62	0.6472019464720195
63	0.6496350364963503
64	0.7420924574209246
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
64	41100.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.46715328467153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35267349260523	41.89294403892944
2	9.046643913538112	9.673965936739659
3	4.036405005688282	6.474452554744525
4	2.2707622298065986	4.856447688564477
5	1.6245733788395906	4.343065693430657
6	1.1831626848691694	3.795620437956204
7	0.7463026166097839	2.793187347931873
8	0.5597269624573379	2.394160583941606
9	0.45051194539249145	2.167883211678832
>10	1.5972696245733788	13.80778588807786
>50	0.07736063708759955	2.6739659367396595
>100	0.05460750853242321	5.126520681265207
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT	337	0.8199513381995134	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC	285	0.6934306569343066	No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	233	0.5669099756690997	No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	209	0.5085158150851582	No Hit
TCGTAAGGGACCATAAAATTTTCCATTTGTTCTGCATTTTGATTCTGAAAAGAAAGCTGGCTTT	182	0.4428223844282238	No Hit
TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA	136	0.3309002433090025	No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA	133	0.3236009732360097	No Hit
TCGTAAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTACACTCAAG	126	0.3065693430656934	No Hit
TCGTAAGGGCCACCCATGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA	125	0.30413625304136255	No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121	0.2944038929440389	No Hit
TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT	115	0.2798053527980535	No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG	105	0.25547445255474455	No Hit
TCGTAAGGGGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGCTT	83	0.20194647201946472	No Hit
TCGTAAGGGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTC	77	0.18734793187347934	No Hit
TCGTAAGGGGATGACAGCATGGAGTGTGTGGACAGTGAGCGGAGGCCGCACTTCCCCCAGTTCT	74	0.18004866180048662	No Hit
TCGTAAGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAA	72	0.1751824817518248	No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA	70	0.17031630170316303	No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.17031630170316303	No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA	69	0.1678832116788321	No Hit
TCGTAAGGGCACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATGAG	68	0.16545012165450124	No Hit
TCGTAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA	66	0.16058394160583941	No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG	63	0.1532846715328467	No Hit
TCGTAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62	0.15085158150851583	No Hit
TCGTAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTGGC	59	0.1435523114355231	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC	58	0.1411192214111922	No Hit
TCGTAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG	54	0.1313868613138686	No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAGAAGGT	52	0.1265206812652068	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC	51	0.12408759124087593	No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG	51	0.12408759124087593	No Hit
TCGTAAGGGATTACTACCTATTAGCATCCCAGGACAAATTCTCGACACCCTACTTTATCTTAAG	49	0.1192214111922141	No Hit
TCGTAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	47	0.11435523114355231	No Hit
TCGTAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA	45	0.10948905109489052	No Hit
TCGTAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44	0.10705596107055962	No Hit
TCGTAAGGGCCACAATTATTGACACAGTTGTTAAATGTCTTTAGTTTTCTGCCATTTAGTGAGA	44	0.10705596107055962	No Hit
TCGTAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC	43	0.10462287104622871	No Hit
TCGTAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG	43	0.10462287104622871	No Hit
TCGTAAGGGGCTCACTTCATGTGTAACAGGTGTCCTCATGTTGTGCTGTATTCTGTGCTTTTTC	43	0.10462287104622871	No Hit
TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42	0.10218978102189782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0024330900243309003	0.0	0.0	0.0	0.0
11	0.004866180048661801	0.0	0.0	0.0	0.0
12	0.004866180048661801	0.0	0.0	0.0	0.0
13	0.004866180048661801	0.0	0.0	0.0	0.0
14	0.0072992700729927005	0.0	0.0	0.0	0.0
15	0.0072992700729927005	0.0	0.0	0.0	0.0
16	0.0072992700729927005	0.0	0.0	0.0	0.0
17	0.009732360097323601	0.0	0.0	0.0	0.0
18	0.009732360097323601	0.0	0.0	0.0	0.0
19	0.009732360097323601	0.0	0.0	0.0	0.0
20	0.009732360097323601	0.0	0.0	0.0	0.0
21	0.012165450121654502	0.0	0.0	0.0	0.0
22	0.021897810218978103	0.0	0.0	0.0	0.0
23	0.024330900243309004	0.0	0.0	0.0	0.0
24	0.024330900243309004	0.0	0.0	0.0	0.0
25	0.0267639902676399	0.0	0.0	0.0	0.0
26	0.0340632603406326	0.0	0.0	0.0	0.0
27	0.0364963503649635	0.0	0.0	0.0	0.0
28	0.043795620437956206	0.0	0.0	0.0	0.0
29	0.04866180048661801	0.0	0.0	0.0	0.0
30	0.051094890510948905	0.0	0.0	0.0	0.0
31	0.0535279805352798	0.0	0.0	0.0	0.0
32	0.06326034063260341	0.0	0.0	0.0	0.0
33	0.06326034063260341	0.0	0.0	0.0	0.0
34	0.0705596107055961	0.0	0.0	0.0	0.0
35	0.08029197080291971	0.0	0.0	0.0	0.0
36	0.08029197080291971	0.0	0.0	0.0	0.0
37	0.0827250608272506	0.0	0.0	0.0	0.0
38	0.0827250608272506	0.0	0.0	0.0	0.0
39	0.09002433090024331	0.0	0.0	0.0	0.0
40	0.0948905109489051	0.0	0.0	0.0	0.0
41	0.10218978102189781	0.0	0.0	0.0	0.0
42	0.10218978102189781	0.0	0.0	0.0	0.0
43	0.10462287104622871	0.0	0.0	0.0	0.0
44	0.10948905109489052	0.0	0.0	0.0	0.0
45	0.11678832116788321	0.0	0.0	0.0	0.0
46	0.1192214111922141	0.0	0.0	0.0	0.0
47	0.12165450121654502	0.0	0.0	0.0	0.0
48	0.13138686131386862	0.0	0.0	0.0	0.0
49	0.13381995133819952	0.0	0.0	0.0	0.0
50	0.1362530413625304	0.0	0.0	0.0	0.0
51	0.1386861313868613	0.0	0.0	0.0	0.0
52	0.15571776155717762	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAAATA	40	6.4028427E-10	58.490124	33
CAGTTAC	20	1.9062322E-4	58.490124	34
AAATATA	40	6.4028427E-10	58.490124	35
TCAGTAC	45	2.7284841E-11	58.490124	36
CTCAGTA	40	6.4028427E-10	58.490124	35
GTTACCT	20	1.9062322E-4	58.490124	36
AGTTACC	20	1.9062322E-4	58.490124	35
ATTAGAT	15	0.004502196	58.49012	35
GGATTAG	15	0.004502196	58.49012	33
CCCATTA	15	0.004502196	58.49012	33
TTAGATG	15	0.004502196	58.49012	36
ACCACAA	15	0.004524283	58.418003	38
GTATCTA	15	0.004524283	58.418003	39
GATGTAG	15	0.004524283	58.418003	39
TCTACGT	30	3.493642E-7	58.418003	38
AGTACGT	45	2.7284841E-11	58.418003	38
TTGATTC	15	0.004524283	58.418003	39
CTACGTA	30	3.493642E-7	58.418003	39
ACCTTAG	20	1.9179228E-4	58.418003	39
CATGATT	40	6.693881E-10	58.20271	32
>>END_MODULE