SRR1526413_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38756
Sequences flagged as poor quality	0
Sequence length	56
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	326	0.8411600784394674	No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	242	0.6244194447311384	No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	242	0.6244194447311384	No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	231	0.5960367426979049	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	205	0.5289503560738982	No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	196	0.5057281453194344	No Hit
TCGTAAGGGACCATAAAATTTTCCATTTGTTCTGCATTTTGATTCTGAAAAGAAAG	164	0.4231602848591186	No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	156	0.4025183197440396	No Hit
TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC	135	0.3483331613169574	No Hit
TCGTAAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA	134	0.3457529156775725	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	128	0.33027144184126334	No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	120	0.30962947672618435	No Hit
TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	120	0.30962947672618435	No Hit
TCGTAAGGGCCACCCATGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAG	119	0.3070492310867995	No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	116	0.29930849416864486	No Hit
TCGTAAGGGGCTCACTTCATGTGTAACAGGTGTCCTCATGTTGTGCTGTATTCTGT	97	0.25028382702033236	No Hit
TCGTAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	97	0.25028382702033236	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	93	0.23996284446279287	No Hit
TCGTAAGGGGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTG	84	0.21674063370832902	No Hit
TCGTAAGGGGATGACAGCATGGAGTGTGTGGACAGTGAGCGGAGGCCGCACTTCCC	82	0.2115801424295593	No Hit
TCGTAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.2115801424295593	No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	76	0.1960986685932501	No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.19093817731448032	No Hit
TCGTAAGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC	73	0.18835793167509546	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	71	0.18319744039632574	No Hit
TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.18319744039632574	No Hit
TCGTAAGGGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTT	70	0.18061719475694085	No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	63	0.16255547528124678	No Hit
TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	63	0.16255547528124678	No Hit
TCGTAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT	62	0.15997522964186192	No Hit
TCGTAAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGC	62	0.15997522964186192	No Hit
TCGTAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT	61	0.15739498400247703	No Hit
TCGTAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	59	0.15223449272370732	No Hit
TCGTAAGGGTCGCTGGGCGTTGAGCAAACTGGGAGGTATAAGCCCACCTGCTGACC	56	0.14449375580555268	No Hit
TCGTAAGGGCACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATT	52	0.13417277324801322	No Hit
TCGTAAGGGGCTCCTCCTCGCCCGGCCCCAGCTGCCAGCACCATGTCCGCAGGGGG	52	0.13417277324801322	No Hit
TCGTAAGGGATTACTACCTATTAGCATCCCAGGACAAATTCTCGACACCCTACTTT	50	0.12901228196924347	No Hit
TCGTAAGGGGTGGTTGTCTTGCCTTCAATAAAATAGTGCATAGATGTGTTAAACTA	50	0.12901228196924347	No Hit
TCGTAAGGGGGGACGCTTTGATGTAAAAATTGAAATTAAGAGCTGATCACAAGCAC	48	0.12385179069047372	No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	48	0.12385179069047372	No Hit
TCGTAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	48	0.12385179069047372	No Hit
TCGTAAGGGGGACTGCTGGTCACAGTTGCAAATCAAAGCATTTGTAACCAAGAGAA	46	0.118691299411704	No Hit
TCGTAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	45	0.11611105377231912	No Hit
TCGTAAGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTAACCA	44	0.11353080813293426	No Hit
TCGTAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCC	43	0.11095056249354937	No Hit
TCGTAAGGGGTAACATAGTTATTTATCAGCACTTTATGGAAGCAAGCATTGTTGAC	43	0.11095056249354937	No Hit
TCGTAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.10837031685416451	No Hit
TCGTAAGGGGAGCCCACCACATATTCACAGTAGGATTAGATGTAGACACACGAGCT	41	0.10579007121477965	No Hit
TCGTAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.10579007121477965	No Hit
TCGTAAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	39	0.1006295799360099	No Hit
TCGTAAGGGGAGCACCCAGGACTTGCTGCTGTCCAGGCCTGTTCCTCCCCCAGTAT	39	0.1006295799360099	No Hit
TCGTAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	39	0.1006295799360099	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTGAT	35	4.5622073E-8	50.72464	37
GCTCGTG	25	1.8819863E-5	50.72464	37
GGCTCGT	25	1.8819863E-5	50.72464	36
CCAGCAC	15	0.007906037	50.724636	35
AAATATA	15	0.007906037	50.724636	35
ATAGGCA	15	0.007906037	50.724636	35
CTCTCCC	20	3.849325E-4	50.724636	36
ACACCAG	15	0.007906037	50.724636	36
TACACCA	15	0.007906037	50.724636	35
AGAGCGT	15	0.007906037	50.724636	37
AATAGGC	15	0.007906037	50.724636	34
CGGGAAG	15	0.007906037	50.724636	34
CACCAGT	15	0.007906037	50.724636	37
TGGATCG	15	0.007906037	50.724636	34
AGGCATT	15	0.007906037	50.724636	37
AGCACCA	15	0.007906037	50.724636	37
TCTCCCG	20	3.849325E-4	50.724636	37
AATATAA	15	0.007906037	50.724636	36
CTCATGT	20	3.849325E-4	50.724636	35
ATCTGAA	15	0.007906037	50.724636	34

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content