##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526413_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38756 Sequences flagged as poor quality 0 Sequence length 56 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.51638455981009 39.0 38.0 40.0 36.0 40.0 2 38.18190731757663 39.0 38.0 40.0 35.0 40.0 3 38.170089792548254 39.0 38.0 40.0 35.0 40.0 4 37.99927753122097 39.0 38.0 40.0 35.0 40.0 5 38.109299205284344 39.0 38.0 40.0 35.0 40.0 6 38.44300237382599 39.0 38.0 40.0 36.0 40.0 7 38.26968727422851 39.0 38.0 40.0 36.0 40.0 8 38.58290329239344 39.0 38.0 40.0 36.0 40.0 9 38.586335019093816 39.0 38.0 40.0 36.0 40.0 10 37.74210444834348 39.0 38.0 40.0 33.0 40.0 11 37.97613272783569 39.0 38.0 40.0 35.0 40.0 12 37.927778924553614 39.0 38.0 40.0 35.0 40.0 13 37.873929198059656 39.0 38.0 40.0 35.0 40.0 14 37.83868304262566 39.0 38.0 40.0 34.0 40.0 15 37.90930436577562 39.0 38.0 40.0 35.0 40.0 16 37.85682216947053 39.0 38.0 40.0 35.0 40.0 17 37.7797760346785 39.0 38.0 40.0 34.0 40.0 18 37.7177211270513 39.0 38.0 40.0 33.0 40.0 19 37.62841882547219 39.0 38.0 40.0 33.0 40.0 20 37.4895242027041 39.0 37.0 40.0 33.0 40.0 21 37.486195685829294 39.0 38.0 40.0 33.0 40.0 22 37.44093817731448 39.0 38.0 40.0 33.0 40.0 23 37.26411394364744 39.0 37.0 40.0 33.0 40.0 24 37.115027350603775 39.0 36.0 40.0 33.0 40.0 25 37.08016823201569 39.0 36.0 40.0 33.0 40.0 26 36.797012075549596 39.0 36.0 40.0 32.0 40.0 27 36.69591805139849 39.0 36.0 40.0 32.0 40.0 28 36.55733305810713 39.0 36.0 40.0 31.0 40.0 29 36.46955310145526 39.0 36.0 40.0 31.0 40.0 30 36.3171379915368 38.0 36.0 40.0 31.0 40.0 31 35.79283207761379 38.0 35.0 40.0 30.0 40.0 32 35.69269274434926 38.0 35.0 40.0 29.0 40.0 33 35.58132934255341 38.0 35.0 40.0 29.0 40.0 34 35.66815460831871 38.0 35.0 40.0 29.0 40.0 35 35.57229848281556 38.0 35.0 40.0 29.0 40.0 36 34.51032098255754 38.0 35.0 40.0 25.0 40.0 37 33.8695685829291 38.0 34.0 40.0 19.0 40.0 38 34.072840334399835 38.0 34.0 40.0 22.0 40.0 39 33.860847352667975 38.0 33.0 40.0 21.0 40.0 40 33.626999690370525 38.0 33.0 40.0 18.0 40.0 41 33.12426462999277 38.0 33.0 39.0 12.0 40.0 42 32.919031891836106 38.0 33.0 39.0 10.0 40.0 43 32.89738363092167 38.0 32.0 39.0 10.0 40.0 44 32.416322633914746 37.0 32.0 39.0 4.0 40.0 45 32.37872845494891 37.0 32.0 39.0 4.0 40.0 46 31.978945195582618 37.0 32.0 39.0 4.0 40.0 47 31.806197750025802 37.0 31.0 39.0 4.0 40.0 48 31.424682629786357 36.0 31.0 39.0 4.0 40.0 49 31.301398493136546 36.0 31.0 39.0 4.0 40.0 50 30.94950459283724 36.0 30.0 39.0 4.0 40.0 51 30.41178140158943 36.0 30.0 39.0 4.0 40.0 52 30.265868510682218 36.0 30.0 39.0 4.0 40.0 53 29.914155227577666 36.0 29.0 39.0 4.0 40.0 54 29.5054443182991 35.0 28.0 39.0 4.0 40.0 55 29.04043244916916 35.0 27.0 39.0 4.0 40.0 56 17.364898338321808 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.22386020561532405 1 2 -0.40183777354768324 1 3 -0.3445487294086078 1 4 -0.18491401189951517 1 5 -1.012518697792892 1 6 -1.160645623065605 1 7 -1.8791725814401516 1 8 -2.27758193356582 1 9 -2.0591030943386173 1 10 -0.5277255967874908 1 11 -0.7110847944965073 1 12 -0.7187433416852969 1 13 -0.16311128397315855 1 14 -0.9901039435550842 1 15 -1.2825021388687787 1 16 -0.4554267745582834 1 17 -0.7231534546267895 1 18 -1.5332425065968849 1 19 -1.4402382224991115 1 20 -1.6698079484440385 1 21 -1.286061975305195 1 22 -0.5314511860967386 1 23 -0.6306995461596827 1 24 -0.8612057296862048 1 25 -1.2283397303435777 1 26 -2.982785033110872 1 27 -0.21127490729433163 1 28 -0.43817098426405465 1 29 -0.2110481145391887 1 30 -0.4726003650784705 1 31 0.10111565610704787 1 32 0.10026439602091841 1 33 0.06505138724970294 1 34 -1.3013385705534546 1 35 -1.4633072113712444 1 36 0.23985484925523082 1 37 0.46504155177943574 1 38 0.8050717192936787 1 39 -0.1966624632007381 1 40 0.30622606475047576 1 41 -0.7184527744334517 1 42 -1.7396794480159876 1 43 -1.965456921028153 1 44 0.15768611790416998 1 45 -2.2526646994116284 1 46 -1.1938671108051437 1 47 -1.6701452829025243 1 48 -2.0573101888801837 1 49 -1.5791214549734534 1 50 -0.8306570455945312 1 51 -0.09402030876597678 1 52 -0.30599255460612085 1 53 -0.16331795322102138 1 54 -0.9433404398594405 1 55 -1.0513731806224769 1 56 0.2607111432452065 2 1 0.7544679677592896 2 2 1.051722597969345 2 3 0.8381138092910803 2 4 0.7670983720014135 2 5 0.8017227882752209 2 6 0.3966299187300635 2 7 0.8065859324917355 2 8 0.624894846619938 2 9 0.5322281750112268 2 10 0.7122124837078658 2 11 0.31987495782547626 2 12 0.6047860700794061 2 13 0.6604181277915444 2 14 0.7126824341538978 2 15 0.5239994091188365 2 16 -1.130349375177481 2 17 0.05703230388714786 2 18 0.398646038294757 2 19 0.620133294528749 2 20 0.322452113475471 2 21 0.02198756029851978 2 22 0.6032237365038853 2 23 -0.047108214890329236 2 24 0.22702956443144018 2 25 0.9125271427214372 2 26 0.5001871031120331 2 27 0.890892275368202 2 28 1.1160085822994148 2 29 0.8075277368540057 2 30 -0.022135968793641325 2 31 1.0020444486767133 2 32 0.2271993805410375 2 33 -0.660954804700765 2 34 -0.561400490058098 2 35 0.2580550177309249 2 36 -2.7183494851100924 2 37 -2.288828417260813 2 38 -1.8992626460313957 2 39 -2.6099751566991927 2 40 -2.1380463810699553 2 41 -1.267988378148619 2 42 -1.6684720176754304 2 43 -1.4066333916163885 2 44 -0.930549176213475 2 45 -1.3269681049843847 2 46 -0.7728144792261951 2 47 -0.7568325894040733 2 48 -1.0836259783538686 2 49 -0.9939821360880039 2 50 -0.8554248474521167 2 51 0.008146873896560436 2 52 -1.0118749075472984 2 53 -1.2685811111157577 2 54 -0.15851071849721166 2 55 -0.7309397440899694 2 56 -1.1587935007176426 3 1 0.7091463303323877 3 2 1.018096866975199 3 3 0.3074120549051145 3 4 0.7480925240481966 3 5 0.972775419854166 3 6 0.7168053573265567 3 7 0.6355333009127904 3 8 0.6848363670877688 3 9 0.5863217422626903 3 10 0.8627972790295075 3 11 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1.0992362035638124 3 53 1.8922376023345358 3 54 0.17920857974840487 3 55 -0.535033311341433 3 56 -1.1412496410685193 4 1 0.570257441443502 4 2 0.7245533234316497 4 3 0.404258901751966 4 4 0.43352795948363365 4 5 0.6281808252595695 4 6 0.33617472669592274 4 7 -0.2496018342223465 4 8 0.22162315387456033 4 9 0.39638180232275033 4 10 0.5287131949454178 4 11 0.5965463660758346 4 12 0.3480293133226482 4 13 -0.16390619653277838 4 14 -0.4893090309954644 4 15 0.23737067512168153 4 16 -0.5969212101703647 4 17 -0.7573346946903783 4 18 -0.6803013301262979 4 19 -1.0761682701938682 4 20 -1.087931954803338 4 21 -0.2582400357185435 4 22 -0.285823205174637 4 23 -0.17441973693869528 4 24 -0.7324298950280195 4 25 -0.8157801119016099 4 26 0.1865313420878536 4 27 -0.09203802335157008 4 28 -0.3221137505597582 4 29 0.3302873385609715 4 30 -0.659404816588804 4 31 -2.3535743279947 4 32 -1.1803397375243918 4 33 -0.338764193036468 4 34 -0.5827376166583846 4 35 -1.0427984673330926 4 36 -0.2347079488369772 4 37 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0.5112749540902968 7 33 0.8026079483356767 7 34 0.9203808864601228 7 35 -0.5590839836186063 7 36 1.3099905958615636 7 37 2.643020435640672 7 38 1.7100490948592935 7 39 2.5884054101024248 7 40 2.515125008943542 7 41 3.63147181078525 7 42 2.8085860120141746 7 43 2.888238403255407 7 44 3.2873995417352404 7 45 2.7978632040574496 7 46 3.3702354532974184 7 47 3.493504792512258 7 48 4.083715452145459 7 49 3.35149694623318 7 50 1.4981514008609729 7 51 1.709901259861475 7 52 2.4624840668116725 7 53 3.041810251907183 7 54 2.211259861799686 7 55 1.8196675433594223 7 56 1.3972118973930208 8 1 -1.1054182342321752 8 2 -0.14031154143321345 8 3 -1.0822275847345253 8 4 -1.0529585270028505 8 5 -0.18262998555123744 8 6 -0.8259874354662387 8 7 -0.033385618006128936 8 8 -0.4540525218011169 8 9 -0.3874019814610321 8 10 -0.41723275100052604 8 11 -0.6466968771674075 8 12 -1.4898085245151904 8 13 -1.19093322355981 8 14 -0.3001198418062714 8 15 -1.0869536492026413 8 16 -0.46178607503523494 8 17 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0.8179925637202885 120 34 0.7709303370095739 120 35 0.04677682224220092 120 36 0.9869136727846453 120 37 1.9675625601827917 120 38 1.1496095344197315 120 39 0.6697240914211093 120 40 0.7070664008849334 120 41 1.239530418843863 120 42 0.7082197116478781 120 43 1.550509465526467 120 44 1.9408793952150987 120 45 1.9985958047900496 120 46 3.091847174909141 120 47 3.092039591047058 120 48 2.699832668262676 120 49 1.3368449315811652 120 50 2.0197631224726997 120 51 1.519425069385285 120 52 0.37383937816698776 120 53 1.3509677610646946 120 54 1.2339704845103086 120 55 0.7713158950077741 120 56 0.8968455970267186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 20.0 5 2.0 6 4.0 7 8.0 8 21.0 9 12.0 10 13.0 11 26.0 12 39.0 13 47.0 14 59.0 15 95.0 16 104.0 17 108.0 18 172.0 19 166.0 20 174.0 21 233.0 22 265.0 23 347.0 24 487.0 25 822.0 26 662.0 27 554.0 28 608.0 29 571.0 30 805.0 31 989.0 32 1278.0 33 1480.0 34 1985.0 35 2618.0 36 3940.0 37 5958.0 38 7697.0 39 6387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.25128443446157 0.562827563059923 0.12134355717346966 0.06454444530503704 8 99.69553101455259 0.2451233357415626 0.018061719475694085 0.04128393023015792 9 99.50975332851687 0.32511095056249356 0.054185158427082256 0.11095056249354937 10 48.196408298069976 33.12777376406234 5.913923005470121 12.761894932397563 11 28.132418206213234 27.67055423676334 20.636804623800188 23.560222933223244 12 37.836722055939724 17.535349365259574 26.158530292083803 18.469398286716896 13 24.403963257302095 17.4450407678811 29.597997729383835 28.552998245432963 14 21.008359995871608 22.623593766126536 33.321292187016205 23.046754050985655 15 24.228506553823927 23.740840127980185 31.290638868820313 20.74001444937558 16 25.384456600268347 26.272061100216742 24.698111260191972 23.645371039322942 17 27.980183713489527 23.454432862008463 22.08690267313448 26.478480751367528 18 27.1828878109196 23.286716895448446 25.76891320053669 23.761482093095264 19 27.180307565280216 23.84404995355558 25.219320879347713 23.756321601816495 20 21.939828671689547 25.647641655485604 28.860047476519764 23.55248219630509 21 22.734544328620085 22.535865414387448 30.924243988027662 23.805346268964804 22 22.81195169780163 22.009495303952935 32.95747755186294 22.221075446382496 23 23.165445350397356 23.89823511198266 31.244194447311386 21.692125090308597 24 21.935234163333764 27.304863888530512 28.582118436330795 22.177783511804925 25 24.800598848764874 26.429364239436254 28.432410108154155 20.337626803644717 26 22.74393391843056 22.731027361899844 29.37790397521941 25.147134744450177 27 19.922520661157026 19.826962809917354 33.87138429752066 26.379132231404963 28 19.11107667673872 21.383745254513055 32.73934040959686 26.765837659151366 29 21.65409260024804 21.462897891690783 33.6890243902439 23.19398511781728 30 22.512669355672767 20.99493225773089 36.27572654876409 20.216671837832248 31 21.806241435478448 19.91105825167412 40.22028595806293 18.062414354784497 32 22.453997256657782 18.080178058438364 39.02016097725096 20.44566370765289 33 22.974581974426673 19.055753999068177 38.8776725164363 19.09199151006885 34 17.83116311579383 21.1240259908359 44.158533744078284 16.886277149291985 35 17.76753579658735 24.727479868465345 41.130473058698634 16.374511276248676 36 21.8546820360057 22.885636575573113 39.878254112161635 15.38142727625955 37 19.30412034009156 23.892740353172005 40.46043165467626 16.34270765206017 38 22.084823974697983 22.172966246694664 40.40286203142013 15.339347747187224 39 19.40786062428705 20.125998133360987 40.124961111687234 20.341180130664732 40 21.339325142218872 20.89253708081149 39.96675065589527 17.80138712107437 41 17.614255330186232 21.414120454427557 40.84401099756187 20.12761321782435 42 16.210886824762596 19.612370920035286 44.15702350682372 20.01971874837839 43 17.18847352024922 19.09657320872274 45.09345794392524 18.621495327102807 44 19.32007064201122 19.07853729482651 42.55142322875545 19.049968834406812 45 18.008777168973484 20.634137474356645 40.06336181152458 21.29372354514529 46 17.39232088117629 20.58762404530576 39.943887359068945 22.076167714449006 47 16.085296485805564 21.26438274330537 42.172930573231866 20.477390197657204 48 18.384705423332033 24.263233190271816 39.292495773182466 18.05956561321368 49 19.430503185541543 23.749837472370302 38.33311662982707 18.486542712261084 50 16.242361201404236 24.316733844753607 39.64373943570407 19.797165518138083 51 15.848173664370208 27.253347198752113 39.828415442610165 17.070063694267514 52 18.369098712446352 26.814930420080636 38.587592664845886 16.22837820262713 53 19.60927135090138 25.58726359876174 38.170182877656664 16.63328217268021 54 18.116300247170546 24.227917262911408 37.96279432808638 19.692988161831664 55 20.67081262522443 25.099529026046678 36.132289037495774 18.09736931123312 56 17.16538301415487 24.130932556203163 35.94920899250624 22.75447543713572 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 10.0 9 19.0 10 39.5 11 60.0 12 84.0 13 117.5 14 127.0 15 269.0 16 411.0 17 535.5 18 660.0 19 619.0 20 578.0 21 660.0 22 836.0 23 930.0 24 1163.5 25 1397.0 26 1505.0 27 1613.0 28 1738.5 29 1858.0 30 1852.0 31 1616.0 32 1380.0 33 1264.0 34 1148.0 35 1078.5 36 1009.0 37 920.0 38 803.0 39 775.0 40 941.0 41 1107.0 42 1184.5 43 1262.0 44 1253.0 45 1244.0 46 1250.5 47 1303.5 48 1350.0 49 1264.5 50 1179.0 51 1345.5 52 1512.0 53 1471.0 54 1492.0 55 1554.0 56 1784.5 57 2015.0 58 2350.5 59 2686.0 60 2405.0 61 2124.0 62 1759.0 63 1196.0 64 998.0 65 938.5 66 879.0 67 634.0 68 389.0 69 337.5 70 286.0 71 255.5 72 198.0 73 171.0 74 130.0 75 89.0 76 69.5 77 50.0 78 35.0 79 19.0 80 18.0 81 12.5 82 7.0 83 5.5 84 4.0 85 2.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.059345649705852 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00258024563938487 25 0.038703684590773044 26 0.04128393023015792 27 0.0928888430178553 28 0.09030859737847043 29 0.13417277324801322 30 0.20641965115078953 31 0.20383940551140467 32 0.3018887398080297 33 0.31478996800495407 34 0.3276911962018784 35 0.3483331613169574 36 0.38961709154711527 37 1.3701104345133657 38 0.4696047063680462 39 0.474765197646816 40 0.6682836206006811 41 0.5212096191557436 42 0.552172566828362 43 0.6089379708948293 44 0.6502219011249871 45 0.6373206729280627 46 0.6760243575188358 47 0.6579626380431417 48 0.8024563938486945 49 0.7766539374548457 50 0.7766539374548457 51 0.750851481060997 52 0.8024563938486945 53 0.8127773764062339 54 0.8282588502425432 55 0.8385798328000826 56 0.8359995871606978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 38756.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.28362060068118 #Duplication Level Percentage of deduplicated Percentage of total 1 69.21609538002981 29.95923211889772 2 12.393442622950818 10.728661368562287 3 5.92548435171386 7.694292496645681 4 3.2548435171385988 5.635256476416555 5 2.4023845007451565 5.199194963360513 6 1.669150521609538 4.334812674166581 7 1.0432190760059614 3.160800908246465 8 0.7570789865871833 2.6215295696150274 9 0.5245901639344263 2.0435545463928166 >10 2.599105812220566 17.955929404479306 >50 0.12518628912071536 3.870368459077304 >100 0.08941877794336811 6.796367014139746 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 326 0.8411600784394674 No Hit TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 242 0.6244194447311384 No Hit TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 0.6244194447311384 No Hit TCGTAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 231 0.5960367426979049 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 205 0.5289503560738982 No Hit TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 196 0.5057281453194344 No Hit TCGTAAGGGACCATAAAATTTTCCATTTGTTCTGCATTTTGATTCTGAAAAGAAAG 164 0.4231602848591186 No Hit TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 156 0.4025183197440396 No Hit TCGTAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC 135 0.3483331613169574 No Hit TCGTAAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCACTACTA 134 0.3457529156775725 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 128 0.33027144184126334 No Hit TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 120 0.30962947672618435 No Hit TCGTAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 120 0.30962947672618435 No Hit TCGTAAGGGCCACCCATGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAG 119 0.3070492310867995 No Hit TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 116 0.29930849416864486 No Hit TCGTAAGGGGCTCACTTCATGTGTAACAGGTGTCCTCATGTTGTGCTGTATTCTGT 97 0.25028382702033236 No Hit TCGTAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 97 0.25028382702033236 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 93 0.23996284446279287 No Hit TCGTAAGGGGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTG 84 0.21674063370832902 No Hit TCGTAAGGGGATGACAGCATGGAGTGTGTGGACAGTGAGCGGAGGCCGCACTTCCC 82 0.2115801424295593 No Hit TCGTAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.2115801424295593 No Hit TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 76 0.1960986685932501 No Hit TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74 0.19093817731448032 No Hit TCGTAAGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC 73 0.18835793167509546 No Hit TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 71 0.18319744039632574 No Hit TCGTAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.18319744039632574 No Hit TCGTAAGGGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTT 70 0.18061719475694085 No Hit TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 63 0.16255547528124678 No Hit TCGTAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 63 0.16255547528124678 No Hit TCGTAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT 62 0.15997522964186192 No Hit TCGTAAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGC 62 0.15997522964186192 No Hit TCGTAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT 61 0.15739498400247703 No Hit TCGTAAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 59 0.15223449272370732 No Hit TCGTAAGGGTCGCTGGGCGTTGAGCAAACTGGGAGGTATAAGCCCACCTGCTGACC 56 0.14449375580555268 No Hit TCGTAAGGGCACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATT 52 0.13417277324801322 No Hit TCGTAAGGGGCTCCTCCTCGCCCGGCCCCAGCTGCCAGCACCATGTCCGCAGGGGG 52 0.13417277324801322 No Hit TCGTAAGGGATTACTACCTATTAGCATCCCAGGACAAATTCTCGACACCCTACTTT 50 0.12901228196924347 No Hit TCGTAAGGGGTGGTTGTCTTGCCTTCAATAAAATAGTGCATAGATGTGTTAAACTA 50 0.12901228196924347 No Hit TCGTAAGGGGGGACGCTTTGATGTAAAAATTGAAATTAAGAGCTGATCACAAGCAC 48 0.12385179069047372 No Hit TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 48 0.12385179069047372 No Hit TCGTAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 48 0.12385179069047372 No Hit TCGTAAGGGGGACTGCTGGTCACAGTTGCAAATCAAAGCATTTGTAACCAAGAGAA 46 0.118691299411704 No Hit TCGTAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 45 0.11611105377231912 No Hit TCGTAAGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTAACCA 44 0.11353080813293426 No Hit TCGTAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCC 43 0.11095056249354937 No Hit TCGTAAGGGGTAACATAGTTATTTATCAGCACTTTATGGAAGCAAGCATTGTTGAC 43 0.11095056249354937 No Hit TCGTAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.10837031685416451 No Hit TCGTAAGGGGAGCCCACCACATATTCACAGTAGGATTAGATGTAGACACACGAGCT 41 0.10579007121477965 No Hit TCGTAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.10579007121477965 No Hit TCGTAAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 39 0.1006295799360099 No Hit TCGTAAGGGGAGCACCCAGGACTTGCTGCTGTCCAGGCCTGTTCCTCCCCCAGTAT 39 0.1006295799360099 No Hit TCGTAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 39 0.1006295799360099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0077407369181546086 0.0 0.0 0.0 0.0 11 0.0077407369181546086 0.0 0.0 0.0 0.0 12 0.0077407369181546086 0.0 0.0 0.0 0.0 13 0.010320982557539478 0.0 0.0 0.0 0.0 14 0.015481473836309217 0.0 0.0 0.0 0.0 15 0.015481473836309217 0.0 0.0 0.0 0.0 16 0.020641965115078955 0.0 0.0 0.0 0.0 17 0.025802456393848695 0.0 0.0 0.0 0.0 18 0.025802456393848695 0.0 0.0 0.0 0.0 19 0.025802456393848695 0.0 0.0 0.0 0.0 20 0.025802456393848695 0.0 0.0 0.0 0.0 21 0.028382702033233564 0.0 0.0 0.0 0.0 22 0.033543193312003304 0.0 0.0 0.0 0.0 23 0.038703684590773044 0.0 0.0 0.0 0.0 24 0.04128393023015791 0.0 0.0 0.0 0.0 25 0.04128393023015791 0.0 0.0 0.0 0.0 26 0.04386417586954278 0.0 0.0 0.0 0.0 27 0.049024667148312516 0.0 0.0 0.0 0.0 28 0.054185158427082256 0.0 0.0 0.0 0.0 29 0.05676540406646713 0.0 0.0 0.0 0.0 30 0.07224687790277634 0.0 0.0 0.0 0.0 31 0.07998761482093095 0.0 0.0 0.0 0.0 32 0.08256786046031582 0.0 0.0 0.0 0.0 33 0.08256786046031582 0.0 0.0 0.0 0.0 34 0.0851481060997007 0.0 0.0 0.0 0.0 35 0.0928888430178553 0.0 0.0 0.0 0.0 36 0.09546908865724017 0.0 0.0 0.0 0.0 37 0.09546908865724017 0.0 0.0 0.0 0.0 38 0.09546908865724017 0.0 0.0 0.0 0.0 39 0.10579007121477965 0.0 0.0 0.0 0.0 40 0.10837031685416451 0.0 0.0 0.0 0.0 41 0.11095056249354938 0.0 0.0 0.0 0.0 42 0.11095056249354938 0.0 0.0 0.0 0.0 43 0.11095056249354938 0.0 0.0 0.0 0.0 44 0.11353080813293426 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGAT 35 4.5622073E-8 50.72464 37 GCTCGTG 25 1.8819863E-5 50.72464 37 GGCTCGT 25 1.8819863E-5 50.72464 36 CCAGCAC 15 0.007906037 50.724636 35 AAATATA 15 0.007906037 50.724636 35 ATAGGCA 15 0.007906037 50.724636 35 CTCTCCC 20 3.849325E-4 50.724636 36 ACACCAG 15 0.007906037 50.724636 36 TACACCA 15 0.007906037 50.724636 35 AGAGCGT 15 0.007906037 50.724636 37 AATAGGC 15 0.007906037 50.724636 34 CGGGAAG 15 0.007906037 50.724636 34 CACCAGT 15 0.007906037 50.724636 37 TGGATCG 15 0.007906037 50.724636 34 AGGCATT 15 0.007906037 50.724636 37 AGCACCA 15 0.007906037 50.724636 37 TCTCCCG 20 3.849325E-4 50.724636 37 AATATAA 15 0.007906037 50.724636 36 CTCATGT 20 3.849325E-4 50.724636 35 ATCTGAA 15 0.007906037 50.724636 34 >>END_MODULE