FastQCFastQC Report
Fri 17 Jun 2016
SRR1526412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346202
Sequences flagged as poor quality0
Sequence length64
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60831.7570666836124575No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36921.0664294255954616No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25340.7319426230928764No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24000.6932368963784149No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18210.5259934951271223No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9330.2694958434671088No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.25880844131460823No Hit
AATAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8720.2518760723508241No Hit
AATAAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8200.23685593959595844No Hit
AATAAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG7920.22876817580487693No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.22876817580487693No Hit
AATAAAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.1842854749539286No Hit
AATAAAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6160.17793080340379316No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6130.17706425728332015No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5720.16522146030352222No Hit
AATAAAGGGGCAGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.16117757840798147No Hit
AATAAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG5430.15684484780561636No Hit
AATAAAGGGGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.1438466559985211No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4680.1351811947937909No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.1337369512596692No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.13084846419142582No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4390.12680458229588507No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4150.11987221333210091No Hit
AATAAAGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4060.11727257497068186No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.11698372626385752No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.1161171801433845No Hit
AATAAAGGGCAGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.11525063402291148No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3780.10918481117960036No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.10629632411135695No Hit
AATAAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG3650.10542977799088393No Hit
AATAAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG3610.10427438316358657No Hit
AATAAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.10283013962946487No Hit
AATAAAGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3530.10196359350899185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGA150.00462571158.18515858
ATTTGGC201.9841004E-458.1429446
TAGCGCA150.00464169158.13450643
AGGTTAC201.9855243E-458.13450243
CGTGCAA150.004647027258.11763842
AAGGTTA201.9883747E-458.11763842
GAAAGGT201.9898011E-458.10920740
ACGTGTG150.004692566657.97467828
CGGCGTA150.004700637457.9495227
ATACGTG150.004706023757.93276226
CCGAACA150.004708718557.9243924
ACATACG150.004708718557.9243924
GCCGAAC150.00471141457.91601623
ACTTAAT150.0047222157.8825520
GGATTAC202.0315226E-457.8658338
GACAGTA258.776489E-657.865839
ATACTTA150.004727614657.8658318
CAGGGTA150.004727614657.8658313
ATATCCG150.004727614657.8658310
TGACAAT150.004727614657.8658311
Produced by FastQC (version 0.11.5)