FastQCFastQC Report
Fri 17 Jun 2016
SRR1526411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340819
Sequences flagged as poor quality0
Sequence length64
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47391.3904741226281399No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29370.8617477312004318No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19770.5800732940358372No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18010.5284329805556616No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14030.4116554534811733No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.22269885188325766No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.22123179752302544No Hit
AATAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6720.1971721060152163No Hit
AATAAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.1915972994463337No Hit
AATAAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG6380.1871961363656369No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5600.16431008834601357No Hit
AATAAAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.16313644485782774No Hit
AATAAAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5450.15990892526531678No Hit
AATAAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4860.14259768381457608No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4860.14259768381457608No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.1405438077102509No Hit
AATAAAGGGGCAGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.11765775969062757No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3880.11384341835402369No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790.11120272050560562No Hit
AATAAAGGGGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.11061589876151272No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.10328062696035138No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.10328062696035138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA258.429703E-658.26029238
CTCACGC150.00463181458.1655656
AATTCGT201.9816852E-458.15696749
AGGATAT201.9831337E-458.1483752
ATGAGCA150.004645384458.122642
GGTTACT258.54879E-658.122644
CATGAGC150.00464810258.11401441
AGTAATG150.00464810258.11401441
GAATGCT201.9976655E-458.06256531
AGCCGTT150.00468080658.01120828
ATGTCTA202.026983E-457.8917214
GTATGTC202.026983E-457.8917212
CTACTAC150.00473844957.8321616
ACTAGTA150.004743966757.81516321
AAGTTAT202.0417696E-457.8066710
AAGTCAC202.0417696E-457.8066710
GACGCAC150.004752251357.7896929
GGACTAA150.00476054757.764248
GGATTAT303.8609323E-757.764248
TACTAGT150.004766083357.74728820
Produced by FastQC (version 0.11.5)