FastQCFastQC Report
Fri 17 Jun 2016
SRR1526410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287706
Sequences flagged as poor quality0
Sequence length56
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49471.719463619111176No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT39401.3694535393770029No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19960.6937637727402279No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17980.6249435187309267No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13920.4838272403078142No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.3246369557812489No Hit
AATAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA9230.32081360833628775No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7540.26207308850006605No Hit
AATAAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG7160.24886516096292743No Hit
AATAAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6810.23669996454714187No Hit
AATAAAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5900.20507045386609943No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5790.20124710642113824No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA5770.2005519523402362No Hit
AATAAAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.1939479885716669No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA5540.19255768040986285No Hit
AATAAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG4790.16648940237603663No Hit
AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.1609281697288204No Hit
AATAAAGGGGCAGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4590.15953786156701633No Hit
AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.15571451412205514No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.14111627842311247No Hit
AATAAAGGGGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.14042112434221046No Hit
AATAAAGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.1393783932208574No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.12790835088597388No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.12512773456236576No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3490.12130438711740457No Hit
AATAAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.11574315447018832No Hit
AATAAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA3240.11261496110612917No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA3230.11226738406567817No Hit
AATAAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC3160.10983434478252103No Hit
AATAAAGGGCAGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.10531584325665785No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.1046206891757558No Hit
AATAAAGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.10357795805440277No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA2920.10149249581169666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAT203.916339E-450.66094637
ATACCTA203.9266123E-450.63406435
CTATACC203.9783007E-450.5000933
CCTAAGA150.00813915950.4378131
GTACTGG204.0026061E-450.43780531
CGCGCTG204.104561E-450.18142340
CCTAATT204.104561E-450.18142338
TAATTCA204.104561E-450.18142340
CTAATTC204.104561E-450.18142339
TCGTAAG150.00830480650.1814240
TCCGAAT204.108113E-450.17262346
CGAATGA204.108113E-450.17262348
CGTAAGG150.00837407850.0760941
TCGCTAT204.2049476E-449.93633330
CGGCATT150.00847879249.9189229
CGCCTAA150.00847879249.9189229
CCGGCAT150.00848463849.91021728
ATTCTGG301.0581371E-649.91021727
GGACTAT150.00859037649.7540868
CCCAGTG150.00859037649.75408614
Produced by FastQC (version 0.11.5)