##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526410_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 287706 Sequences flagged as poor quality 0 Sequence length 56 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.07869839349892 39.0 38.0 40.0 35.0 40.0 2 37.945687611659125 39.0 38.0 40.0 35.0 40.0 3 37.61340048521755 39.0 38.0 40.0 33.0 40.0 4 37.39586244291047 39.0 38.0 40.0 33.0 40.0 5 37.69848734471996 39.0 38.0 40.0 34.0 40.0 6 37.21336364205126 39.0 35.0 40.0 31.0 40.0 7 36.76362675787088 39.0 35.0 40.0 31.0 40.0 8 37.80170382265229 39.0 38.0 40.0 34.0 40.0 9 37.748364650024676 39.0 38.0 40.0 34.0 40.0 10 36.82399741402682 39.0 36.0 40.0 31.0 40.0 11 37.13650045532592 39.0 37.0 40.0 33.0 40.0 12 36.95018525856256 39.0 36.0 40.0 32.0 40.0 13 36.8985839711372 39.0 36.0 40.0 32.0 40.0 14 36.94355696440116 39.0 36.0 40.0 32.0 40.0 15 36.91736703440318 39.0 36.0 40.0 32.0 40.0 16 36.737266515123075 39.0 36.0 40.0 31.0 40.0 17 36.74813872494838 39.0 36.0 40.0 31.0 40.0 18 36.65362905187935 39.0 36.0 40.0 31.0 40.0 19 36.537580029613565 39.0 36.0 40.0 31.0 40.0 20 36.63816882512009 39.0 36.0 40.0 31.0 40.0 21 36.55111815533913 39.0 36.0 40.0 31.0 40.0 22 36.51860927474575 39.0 36.0 40.0 31.0 40.0 23 36.31781054270679 39.0 36.0 40.0 30.0 40.0 24 36.25954272764558 39.0 36.0 40.0 30.0 40.0 25 36.244756800344796 39.0 36.0 40.0 30.0 40.0 26 36.01245716112977 39.0 35.0 40.0 30.0 40.0 27 35.90009940703357 39.0 35.0 40.0 29.0 40.0 28 35.681490827441905 38.0 35.0 40.0 29.0 40.0 29 35.73545563874233 39.0 35.0 40.0 29.0 40.0 30 35.49627049835596 38.0 35.0 40.0 29.0 40.0 31 35.123594919813975 38.0 35.0 40.0 27.0 40.0 32 34.866982266619395 38.0 35.0 40.0 26.0 40.0 33 34.898736905035 38.0 35.0 40.0 26.0 40.0 34 34.91881990643226 38.0 35.0 40.0 26.0 40.0 35 34.69537653020792 38.0 35.0 40.0 26.0 40.0 36 34.25792301863708 38.0 34.0 40.0 23.0 40.0 37 33.81407061375154 38.0 34.0 40.0 17.0 40.0 38 33.90028362286501 38.0 34.0 40.0 20.0 40.0 39 33.76992832961426 38.0 33.0 40.0 19.0 40.0 40 33.29922212258347 38.0 33.0 40.0 11.0 40.0 41 32.96658046756063 38.0 33.0 40.0 4.0 40.0 42 32.87739219898091 38.0 33.0 40.0 4.0 40.0 43 32.71271714875602 38.0 32.0 40.0 4.0 40.0 44 32.22904631811641 38.0 32.0 40.0 4.0 40.0 45 32.24404079164147 38.0 32.0 40.0 4.0 40.0 46 31.882581524194837 38.0 32.0 40.0 4.0 40.0 47 31.68943643858661 37.0 31.0 40.0 4.0 40.0 48 31.24357156263686 37.0 31.0 40.0 4.0 40.0 49 31.16852620383308 37.0 31.0 40.0 4.0 40.0 50 30.865192244861074 37.0 30.0 39.0 4.0 40.0 51 30.295555184806712 36.0 30.0 39.0 4.0 40.0 52 30.093991783278764 36.0 30.0 39.0 4.0 40.0 53 29.709867017024322 36.0 29.0 39.0 4.0 40.0 54 29.284665596129383 36.0 27.0 39.0 4.0 40.0 55 28.742059602510896 35.0 23.0 39.0 4.0 40.0 56 16.845004970351678 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.1864200239080134 1 2 -0.46961956261715443 1 3 -0.8934148932381021 1 4 -0.8976022925953302 1 5 -0.8288243204827666 1 6 -1.2296635682183066 1 7 -1.9060672247495916 1 8 -1.8808151192423281 1 9 -1.9396724846718882 1 10 0.029079145877147994 1 11 -0.7450880394959682 1 12 -0.9685249578224955 1 13 -0.6571128265870243 1 14 -0.8835368025281554 1 15 -0.6521853844281225 1 16 -0.7312531732101846 1 17 -0.8065679559643399 1 18 -0.6213472698951463 1 19 -0.778566862631088 1 20 -0.80994283406487 1 21 -0.9039296547998319 1 22 -0.9472446552822191 1 23 -0.5090959182537276 1 24 -1.2040449163649924 1 25 -0.7531400509108721 1 26 -0.86735100492497 1 27 -1.3452489672846966 1 28 -1.2147781318320128 1 29 -0.62318096858845 1 30 -0.6107684997166984 1 31 -0.5656795950657383 1 32 -0.5643777137263228 1 33 -0.3712500817052984 1 34 -0.10135174728228691 1 35 -0.42416147255851655 1 36 -0.06486768685229549 1 37 0.0063560655307384195 1 38 -0.19977701481631271 1 39 -0.06081302189289772 1 40 -0.16526262385993817 1 41 0.0701087857820184 1 42 -0.01273939261395185 1 43 0.30012054589396797 1 44 0.3926552997082595 1 45 -0.17507488768939794 1 46 -0.7184677393781485 1 47 -0.5358480391899931 1 48 -0.3466489999783313 1 49 -0.40976361313655474 1 50 -0.7871391984456508 1 51 -0.6675252327792371 1 52 -0.985101134410499 1 53 -0.8991843802055044 1 54 -0.6175895086809469 1 55 -0.9948725505109444 1 56 0.41995882618709146 2 1 -0.21237787831414323 2 2 0.43752329452570393 2 3 0.22908100167158096 2 4 0.13718150598157308 2 5 0.6019228059540112 2 6 0.08437584064868275 2 7 0.5897318994978136 2 8 0.1206900805387292 2 9 -0.018873360424485952 2 10 0.7432553910878781 2 11 0.08274447281930009 2 12 0.10342359171226434 2 13 -0.2066749502870806 2 14 -0.32497084741047644 2 15 -0.47804745339363564 2 16 -0.5718388327613582 2 17 -0.40935941737649273 2 18 -0.5092098862060439 2 19 -0.5933588713886166 2 20 -0.7036346786187835 2 21 -0.47468772814411153 2 22 -0.37202571713224586 2 23 -0.36071058710977866 2 24 -0.7865845989046747 2 25 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0.6021350429368582 3 10 0.9167848028525825 3 11 0.3645722039117416 3 12 0.3442849362500766 3 13 0.36191748668770884 3 14 0.3740837744382617 3 15 0.11607019366518756 3 16 -0.09232202603867279 3 17 0.2968380616151052 3 18 -0.018138457634613303 3 19 -0.009325257943238796 3 20 -0.1055254349213044 3 21 -0.0856120978920103 3 22 -0.575597145703675 3 23 0.11397428683980593 3 24 -0.1754501451231647 3 25 -0.20513464185069807 3 26 -0.02997496255400023 3 27 0.2410283597380669 3 28 -0.26433301125504727 3 29 -0.002829379677024235 3 30 -0.18243854704597595 3 31 -0.027773915552550932 3 32 -0.009667323823236984 3 33 -0.29886164357142775 3 34 -0.05560011329536252 3 35 -0.5950680535817341 3 36 0.09313603187657549 3 37 0.13542694675679456 3 38 0.12009001245436224 3 39 -0.04199400003128062 3 40 -0.007723746239925333 3 41 0.19123510987487435 3 42 -0.03661532229617137 3 43 -0.09822011220835236 3 44 0.04367231571006869 3 45 0.41307588986300203 3 46 0.1826056210004836 3 47 0.2396055330434983 3 48 0.5069119646373998 3 49 0.328361809446271 3 50 0.7029864495146825 3 51 0.4237385715930877 3 52 0.4691472316025731 3 53 0.13622806063289872 3 54 0.16221666666275425 3 55 0.041469572545175026 3 56 -0.35501581884333433 4 1 0.32349168690324603 4 2 0.8057427562234238 4 3 0.13394642610222718 4 4 0.21854796560890577 4 5 0.7928648349395218 4 6 0.5207112443753914 4 7 0.5634896634729643 4 8 0.42607310331305825 4 9 0.2726380267390738 4 10 0.47731336210237174 4 11 -0.08454331185978958 4 12 -0.6746302385154834 4 13 -0.29062940163283457 4 14 -0.5004366859197944 4 15 -0.6635546997704509 4 16 -0.5112798265501795 4 17 -0.6863780509168649 4 18 -0.5896964286491055 4 19 -0.1117336125894397 4 20 -0.7694214281011895 4 21 -0.37173741758510204 4 22 -0.5647275804862844 4 23 -0.5235470260331709 4 24 -0.5832719695050912 4 25 -0.329601820026312 4 26 -0.4146190375753136 4 27 -0.7507129019840093 4 28 -0.7744566259182974 4 29 -1.031601758193645 4 30 -0.6481248215557827 4 31 -0.6119064816224551 4 32 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-1.3924377535188484 120 54 -1.7050041347012694 120 55 -1.62818089633803 120 56 -0.7871876005995162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 801.0 5 182.0 6 248.0 7 384.0 8 572.0 9 689.0 10 434.0 11 708.0 12 638.0 13 650.0 14 879.0 15 1024.0 16 1083.0 17 1293.0 18 1508.0 19 1829.0 20 1785.0 21 2169.0 22 2518.0 23 2578.0 24 3079.0 25 3827.0 26 4180.0 27 4559.0 28 5668.0 29 6207.0 30 6717.0 31 8425.0 32 10542.0 33 12307.0 34 15776.0 35 18874.0 36 22901.0 37 35625.0 38 46665.0 39 60382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.05585906392686 0.6572472814652789 0.1815254396427913 0.10536821496506853 8 99.16442479475576 0.5561232647216255 0.12895108200732694 0.1505008585152899 9 97.48006645673013 1.4299319444154797 0.40875059957039483 0.6812509992839914 10 39.795833246439074 37.31691379394243 8.489916790056515 14.397336169561983 11 29.39146211757836 38.08853482374368 16.511647306625512 16.00835575205244 12 45.06753421895963 26.108596970518516 15.422688438892482 13.401180371629371 13 28.087353061806148 25.670302322509787 21.92515971165009 24.31718490403398 14 27.917040311985154 37.40207016885292 18.705901162992777 15.974988356169145 15 33.142165961085276 30.907940745066142 20.2015251680535 15.748368125795082 16 23.939716238104175 32.80536380888824 25.86007938659604 17.394840566411546 17 32.10325818717719 30.991011657733935 17.742417606862563 19.163312548226312 18 25.64492919855686 32.99340298777224 21.27623337712804 20.085434436542858 19 21.04439949114721 35.73752372213301 24.31718490403398 18.900891882685798 20 21.938367639187227 38.05968592938625 18.061493329996594 21.94045310142993 21 21.111134282913806 33.79734868233544 25.503117766052846 19.588399268697906 22 22.5111746018505 36.23699192926112 19.95092212188832 21.300911347000064 23 22.523843950727134 40.64455134443734 20.582265105803184 16.24933959903234 24 19.773657831258298 41.69464661842298 23.009600077857257 15.522095472461473 25 20.983799234297358 39.86278553023691 20.871830001495244 18.28158523397049 26 22.693853986979022 39.45801569194814 22.540829670023925 15.307300651048914 27 19.75605936700712 38.17653506864092 24.787639413289718 17.279766151062237 28 21.214093083949543 41.08568943018704 25.073858199217053 12.626359286646368 29 23.69129222381975 37.822237849391996 25.79782697241766 12.6886429543706 30 20.620834073748874 40.46397050881356 27.31384211204839 11.60135330538918 31 23.359858963549325 37.2495801657039 28.597857974064343 10.792702896682439 32 19.18491734456559 36.74880488714857 33.48477821983103 10.58149954845481 33 20.58815323233169 37.18646243129377 30.372338941435785 11.853045394938757 34 19.165649307614498 38.26223446928738 32.24493355192019 10.327182671177928 35 17.23776992348461 41.42158829625029 32.201361436661095 9.139280343604005 36 16.791034357592597 39.4696980273353 32.50254785073085 11.23671976434126 37 15.253699676817945 39.692888937446476 34.53924529764821 10.514166088087377 38 15.292399907784523 40.28488993523959 34.77012497991519 9.652585177060702 39 14.716278614889172 40.23897842138721 34.172656844870104 10.872086118853518 40 14.323420790086288 40.460679467208095 35.374302067106576 9.841597675599042 41 13.25305837119888 42.02901083537225 34.22719328905977 10.490737504369102 42 13.227818450070972 40.876703516463536 34.34897526798263 11.546502765482858 43 14.105596059389036 40.64139880494256 34.73572997858972 10.517275157078686 44 13.468813539154992 41.41186845798235 34.75707701514266 10.362240987719993 45 13.30512360132579 42.9085423688475 33.85167840905511 9.934655620771602 46 13.075247885272534 42.90550949526637 33.40043975127444 10.618802868186657 47 13.461794252511464 42.5667681752949 34.018691588785046 9.95274598340859 48 14.255016335516077 42.67706157017261 33.00475342484962 10.0631686694617 49 13.39287904325065 43.87682517879482 32.98269343303561 9.747602344918933 50 12.981470060551692 44.18136073110563 33.05498710473201 9.782182103610674 51 12.632556184203612 44.261416384942805 33.457583772705775 9.648443658147803 52 13.442179350569234 44.175522965621674 31.978366328304634 10.403931355504458 53 13.415788477314319 44.35504792354669 32.08318433913184 10.14597926000715 54 13.393808909335728 44.64801671903052 31.728638352782767 10.229536018850988 55 13.528176101952285 44.89028696875011 30.828713006701662 10.75282392259594 56 13.638069188013958 45.24257885785423 30.286676134869282 10.832675819262533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 197.0 1 126.5 2 56.0 3 108.5 4 1082.5 5 2004.0 6 2535.0 7 3066.0 8 5057.0 9 7048.0 10 5562.0 11 4076.0 12 3957.0 13 4327.5 14 4817.0 15 5643.0 16 6469.0 17 7936.0 18 9403.0 19 10738.0 20 12073.0 21 13281.5 22 15567.5 23 16645.0 24 17335.5 25 18026.0 26 17818.0 27 17610.0 28 15780.0 29 14673.0 30 15396.0 31 12918.0 32 10440.0 33 9548.5 34 8657.0 35 8355.0 36 8053.0 37 7975.0 38 7813.5 39 7730.0 40 7955.5 41 8181.0 42 8829.0 43 9477.0 44 10294.0 45 11111.0 46 10936.0 47 10775.5 48 10790.0 49 10255.0 50 9720.0 51 8766.5 52 7813.0 53 7393.0 54 6452.0 55 5931.0 56 5316.5 57 4702.0 58 3938.5 59 3175.0 60 2733.5 61 2292.0 62 1896.5 63 1308.5 64 1116.0 65 952.0 66 788.0 67 634.5 68 481.0 69 402.5 70 324.0 71 254.5 72 148.5 73 112.0 74 87.0 75 62.0 76 55.5 77 49.0 78 37.5 79 21.0 80 16.0 81 10.5 82 5.0 83 4.5 84 4.0 85 5.0 86 6.0 87 3.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.049703516784495284 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 6.95154080902032E-4 24 0.0 25 0.04414228413727903 26 0.05908809687667271 27 0.11852377079379646 28 0.11504800038928628 29 0.1588427074861143 30 0.24504181351796625 31 0.23913300383029898 32 0.31733783793177756 33 0.33993034556109364 34 0.35313827309823226 35 0.38129201337476454 36 0.413616678136709 37 1.3781429653882782 38 0.49355939744044264 39 0.48799816479322644 40 0.7094047395605236 41 0.5582087269643317 42 0.5839294279577069 43 0.6471884493197917 44 0.6802082681626382 45 0.6913307334570707 46 0.7264360145426234 47 0.7007153135492482 48 0.8473928246195769 49 0.8074214649677102 50 0.8098545042508672 51 0.7858716884597472 52 0.8376606674869485 53 0.8550395195094993 54 0.8765892960174623 55 0.8873641842714438 56 0.8831932597860316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 287706.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.54501087611466 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61164268732983 41.55716251806202 2 12.086978301122906 12.943947691859762 3 4.470489397294906 7.181172101991332 4 1.9734010419986143 4.226631210270073 5 1.039257610931643 2.7823530040209885 6 0.6521806306801442 2.0952611377775785 7 0.40871904448647023 1.531940597761227 8 0.2941581589608988 1.2600561456687354 9 0.24919457503804648 1.2008813607612874 >10 1.0979881890216756 10.017070070949705 >50 0.05074578387150266 1.8561539570656076 >100 0.05535903695073017 5.958395354572772 >500 0.006590361541753591 2.4228808116373504 >1k 0.0032951807708767955 4.966094037601577 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4947 1.719463619111176 No Hit AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 3940 1.3694535393770029 No Hit AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1996 0.6937637727402279 No Hit AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1798 0.6249435187309267 No Hit AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1392 0.4838272403078142 No Hit AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.3246369557812489 No Hit AATAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 923 0.32081360833628775 No Hit AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 754 0.26207308850006605 No Hit AATAAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 716 0.24886516096292743 No Hit AATAAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 681 0.23669996454714187 No Hit AATAAAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 590 0.20507045386609943 No Hit AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 579 0.20124710642113824 No Hit AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA 577 0.2005519523402362 No Hit AATAAAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 558 0.1939479885716669 No Hit AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 554 0.19255768040986285 No Hit AATAAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 479 0.16648940237603663 No Hit AATAAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 463 0.1609281697288204 No Hit AATAAAGGGGCAGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 459 0.15953786156701633 No Hit AATAAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 448 0.15571451412205514 No Hit AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 406 0.14111627842311247 No Hit AATAAAGGGGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 404 0.14042112434221046 No Hit AATAAAGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 401 0.1393783932208574 No Hit AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 368 0.12790835088597388 No Hit AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 360 0.12512773456236576 No Hit AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 349 0.12130438711740457 No Hit AATAAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 333 0.11574315447018832 No Hit AATAAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 324 0.11261496110612917 No Hit AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA 323 0.11226738406567817 No Hit AATAAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 316 0.10983434478252103 No Hit AATAAAGGGCAGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 303 0.10531584325665785 No Hit AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 301 0.1046206891757558 No Hit AATAAAGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 298 0.10357795805440277 No Hit AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 292 0.10149249581169666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.024330392831571117 0.0 0.0 0.0 0.0 7 0.024330392831571117 0.0 0.0 0.0 0.0 8 0.027458586195630262 0.0 0.0 0.0 0.0 9 0.034062549964199565 0.0 0.0 0.0 0.0 10 0.08376606674869484 0.0 0.0 0.0 0.0 11 0.1265180427241698 0.0 0.0 0.0 0.0 12 0.13972597026130842 0.0 0.0 0.0 0.0 13 0.15849513044566327 0.0 0.0 0.0 0.0 14 0.18873433296490166 0.0 0.0 0.0 0.0 15 0.20993653243241364 0.0 0.0 0.0 0.0 16 0.22905326965721953 0.0 0.0 0.0 0.0 17 0.24226119719435812 0.0 0.0 0.0 0.0 18 0.25824974105510484 0.0 0.0 0.0 0.0 19 0.2801470946035189 0.0 0.0 0.0 0.0 20 0.30969114304185524 0.0 0.0 0.0 0.0 21 0.32533210986215094 0.0 0.0 0.0 0.0 22 0.34097307668244664 0.0 0.0 0.0 0.0 23 0.3663462006353708 0.0 0.0 0.0 0.0 24 0.38928628530513787 0.0 0.0 0.0 0.0 25 0.4087505995703948 0.0 0.0 0.0 0.0 26 0.435861608725574 0.0 0.0 0.0 0.0 27 0.46227746379985124 0.0 0.0 0.0 0.0 28 0.5057245938562283 0.0 0.0 0.0 0.0 29 0.5276219474046422 0.0 0.0 0.0 0.0 30 0.5543853795193705 0.0 0.0 0.0 0.0 31 0.5714166545014703 0.0 0.0 0.0 0.0 32 0.5846245820386089 0.0 0.0 0.0 0.0 33 0.6037413192634148 0.0 0.0 0.0 0.0 34 0.6218153253668676 0.0 0.0 0.0 0.0 35 0.6656100324636955 0.0 0.0 0.0 0.0 36 0.6861170778503055 0.0 0.0 0.0 0.0 37 0.6996725824278951 0.0 0.0 0.0 0.0 38 0.717051434450446 0.0 0.0 0.0 0.0 39 0.7340827094325457 0.0 0.0 0.0 0.0 40 0.7525042925764496 0.0 0.0 0.0 0.0 41 0.7684928364371963 0.0 0.0 0.0 0.0 42 0.7907377670260614 0.0 0.0 0.0 0.0 43 0.8115923894531223 0.0 0.0 0.0 0.0 44 0.826885779232967 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTAAT 20 3.916339E-4 50.660946 37 ATACCTA 20 3.9266123E-4 50.634064 35 CTATACC 20 3.9783007E-4 50.50009 33 CCTAAGA 15 0.008139159 50.43781 31 GTACTGG 20 4.0026061E-4 50.437805 31 CGCGCTG 20 4.104561E-4 50.181423 40 CCTAATT 20 4.104561E-4 50.181423 38 TAATTCA 20 4.104561E-4 50.181423 40 CTAATTC 20 4.104561E-4 50.181423 39 TCGTAAG 15 0.008304806 50.18142 40 TCCGAAT 20 4.108113E-4 50.172623 46 CGAATGA 20 4.108113E-4 50.172623 48 CGTAAGG 15 0.008374078 50.07609 41 TCGCTAT 20 4.2049476E-4 49.936333 30 CGGCATT 15 0.008478792 49.91892 29 CGCCTAA 15 0.008478792 49.91892 29 CCGGCAT 15 0.008484638 49.910217 28 ATTCTGG 30 1.0581371E-6 49.910217 27 GGACTAT 15 0.008590376 49.754086 8 CCCAGTG 15 0.008590376 49.754086 14 >>END_MODULE