FastQCFastQC Report
Fri 17 Jun 2016
SRR1526409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35538
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11953.3625977826551865No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7252.0400697844560756No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.43052507175417865No Hit
ATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.4248972930384377No Hit
ATTCCAGGGGCTATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAA1470.41364173560695594No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1270.357363948449547No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC1250.35173616973380606No Hit
ATTCCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.34329450166019476No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.34329450166019476No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.3067139400078789No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1070.301086161292138No Hit
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.2729472677134335No Hit
ATTCCAGGGGAAACCCTGCTTGCTGGCTAGCTTACTGGCTCACCATGGCCAGCTAGTTTTCTTT800.22511114862963588No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.2222972592717654No Hit
ATTCCAGGGCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT720.20260003376667227No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1969722550509314No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG690.19415836569306094No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACG680.1913444763351905No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.18571669761944962No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG590.16601947211435647No Hit
ATTCCAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.163205582756486No Hit
ATTCCAGGGATCTTCACACACTGTAAGCCAAAGCAAATATCCCCCTTCTTACGTTGTCTTTGTC550.15476391468287465No Hit
ATTCCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.14913613596713376No Hit
ATTCCAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.14350835725139288No Hit
ATTCCAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.1322527998199111No Hit
ATTCCAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.1266250211041702No Hit
ATTCCAGGGGTGCAGTGCTATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.1153694636726884No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.1153694636726884No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG390.1097416849569475No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.10692779559907704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCACT201.9304405E-458.31960358
CTGACGG351.513763E-858.31960358
TATTTCA201.9304405E-458.31960356
TTATTTC258.224739E-658.319655
AGTTTTC258.224739E-658.319655
CAGCTAG258.294473E-658.23687750
GCTAGTT258.294473E-658.23687752
AGCTAGT258.294473E-658.23687751
GGATCAT201.9440717E-458.23687748
CATTTAT201.9440717E-458.23687752
GGCCAGC258.294473E-658.23687747
CTAGTTT258.294473E-658.23687753
TCATTTA201.9440717E-458.23687751
TGGCCAG258.294473E-658.23687746
TAAGAGT150.004601080458.15439238
AGCATTT150.004601080458.15439244
GCATTTG150.004601080458.15439245
TGCGAAA201.9577795E-458.1543944
TTCTATA201.9577795E-458.1543941
TCTCTTC201.9577795E-458.1543937
Produced by FastQC (version 0.11.5)