FastQCFastQC Report
Fri 17 Jun 2016
SRR1526408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34512
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9442.735280482151136No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5831.6892675011590172No Hit
ATTCCAGGGGCTATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAA1120.3245248029670839No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC1070.3100370885489105No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1050.30424200278164115No Hit
ATTCCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.2897542883634678No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.2665739452943904No Hit
ATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.260778859527121No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.25498377375985165No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.24049605934167825No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG770.22311080203987016No Hit
ATTCCAGGGGAAACCCTGCTTGCTGGCTAGCTTACTGGCTCACCATGGCCAGCTAGTTTTCTTT730.2115206305053315No Hit
ATTCCAGGGCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT680.19703291608715812No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.19703291608715812No Hit
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.17675011590171535No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACG570.16515994436717663No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.16226240148354196No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG510.14777468706536856No Hit
ATTCCAGGGATCTTCACACACTGTAAGCCAAAGCAAATATCCCCCTTCTTACGTTGTCTTTGTC480.13908205841446453No Hit
ATTCCAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1332869726471952No Hit
ATTCCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.12749188687992583No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG440.12749188687992583No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.11879925822902179No Hit
ATTCCAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.10720908669448308No Hit
ATTCCAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1043115438108484No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.10141400092721373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTTG150.004503090458.4669634
GCATCGG150.004503090458.4669633
CATCGGA150.004503090458.4669634
GTTGATG150.004555793558.29575737
TGGTTGA150.004555793558.29575735
TTGATGC150.004555793558.29575738
ATCCTGG351.5344995E-858.2105353
TCCTGGC351.5344995E-858.2105354
GTGCATC150.004582316658.21052631
TTACTGG150.004582316658.21052632
ACTGACG150.004582316658.21052658
AACTGAC150.004582316658.21052657
TAACTGG150.004582316658.21052631
CTTACTG150.004582316658.21052631
TCATGGC201.947598E-458.21052654
ATCATGG201.947598E-458.21052653
CATGGCT201.947598E-458.21052655
ATCGAAC150.004582316658.21052653
TGATCCT351.5525075E-858.1255551
GATCCTG351.5525075E-858.1255552
Produced by FastQC (version 0.11.5)