##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526408_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34512 Sequences flagged as poor quality 0 Sequence length 64 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.1987424663885 39.0 38.0 40.0 35.0 40.0 2 38.08168173388966 39.0 38.0 40.0 35.0 40.0 3 38.153656699119146 39.0 38.0 40.0 35.0 40.0 4 37.68193671766342 39.0 38.0 40.0 33.0 40.0 5 38.14113931386184 39.0 38.0 40.0 35.0 40.0 6 37.6438919796013 39.0 38.0 40.0 33.0 40.0 7 38.11074408901252 39.0 38.0 40.0 35.0 40.0 8 37.95694251274919 39.0 38.0 40.0 35.0 40.0 9 38.08933124710246 39.0 38.0 40.0 35.0 40.0 10 37.39919448307835 39.0 38.0 40.0 33.0 40.0 11 37.38270746407047 39.0 38.0 40.0 33.0 40.0 12 37.300591098748264 39.0 37.0 40.0 32.0 40.0 13 37.136764024107556 39.0 37.0 40.0 32.0 40.0 14 37.257881316643484 39.0 37.0 40.0 33.0 40.0 15 37.020659480760315 39.0 37.0 40.0 32.0 40.0 16 36.87989684747334 39.0 36.0 40.0 32.0 40.0 17 36.75040565600371 39.0 37.0 40.0 31.0 40.0 18 36.87381200741771 39.0 36.0 40.0 31.0 40.0 19 36.764226935558646 39.0 36.0 40.0 31.0 40.0 20 36.820091562355124 39.0 36.0 40.0 31.0 40.0 21 36.840374362540565 39.0 36.0 40.0 32.0 40.0 22 36.657017848864164 39.0 36.0 40.0 31.0 40.0 23 36.45190078813167 38.0 36.0 40.0 31.0 40.0 24 36.34570584144645 38.0 36.0 40.0 30.0 40.0 25 36.3160060268892 39.0 36.0 40.0 30.0 40.0 26 36.55021441817339 39.0 36.0 40.0 31.0 40.0 27 36.09428604543347 39.0 36.0 40.0 29.0 40.0 28 35.9598980064905 39.0 36.0 40.0 29.0 40.0 29 35.98438224385721 39.0 36.0 40.0 29.0 40.0 30 35.84703871117293 39.0 35.0 40.0 29.0 40.0 31 35.76483541956421 39.0 36.0 40.0 29.0 40.0 32 35.770022021325914 39.0 35.0 40.0 29.0 40.0 33 35.812268196569306 39.0 35.0 40.0 29.0 40.0 34 35.34593764487715 39.0 35.0 40.0 27.0 40.0 35 35.400121696801115 39.0 35.0 40.0 27.0 40.0 36 35.10161682892907 39.0 35.0 40.0 26.0 40.0 37 34.98052851182197 39.0 35.0 40.0 26.0 40.0 38 35.007446685210944 39.0 35.0 40.0 26.0 40.0 39 34.54131896152063 39.0 35.0 40.0 23.0 40.0 40 34.741481223922115 39.0 35.0 40.0 25.0 40.0 41 34.66017617060732 39.0 35.0 40.0 25.0 40.0 42 34.38180922577654 39.0 35.0 40.0 22.0 40.0 43 34.44772832637923 39.0 35.0 40.0 23.0 40.0 44 34.215374362540565 39.0 34.0 40.0 22.0 40.0 45 34.08550649049606 39.0 34.0 40.0 21.0 40.0 46 33.95589939731108 39.0 35.0 40.0 17.0 40.0 47 33.815339592025964 39.0 34.0 40.0 16.0 40.0 48 33.68990496059342 39.0 34.0 40.0 15.0 40.0 49 33.400875057950856 39.0 33.0 40.0 11.0 40.0 50 33.08738989337042 38.0 33.0 40.0 4.0 40.0 51 32.63685095039406 38.0 33.0 40.0 4.0 40.0 52 32.52436833565137 38.0 33.0 40.0 4.0 40.0 53 32.12789754288364 38.0 32.0 40.0 4.0 40.0 54 31.72580551692165 38.0 32.0 39.0 4.0 40.0 55 31.25657742234585 37.0 31.0 39.0 4.0 40.0 56 30.279844691701438 37.0 30.0 39.0 4.0 40.0 57 29.834955957348168 36.0 28.0 39.0 4.0 40.0 58 29.533292767732963 36.0 27.0 39.0 4.0 40.0 59 29.045984005563284 36.0 24.0 39.0 4.0 40.0 60 28.580290913305518 35.0 21.0 39.0 4.0 40.0 61 27.971401251738527 35.0 4.0 39.0 4.0 40.0 62 27.759880621233194 35.0 4.0 39.0 4.0 40.0 63 27.3419969865554 35.0 4.0 39.0 4.0 40.0 64 27.276396615669913 35.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.5878548025677901 2 2 0.11845884868323253 2 3 -0.15231624799575627 2 4 0.019693105214287243 2 5 -0.1520111548275267 2 6 0.2115048542246214 2 7 -0.09375428863083357 2 8 0.25800971653596605 2 9 -0.13043364489806208 2 10 -0.7380745496465835 2 11 -1.0344435888371137 2 12 -0.6292766798477132 2 13 -0.41985683640630356 2 14 -0.8253196133167364 2 15 -2.018551766637252 2 16 -2.70429387067972 2 17 -1.0613969574317679 2 18 -1.615371086076756 2 19 -1.5820404417978793 2 20 -2.2742419954951956 2 21 -1.7652016338451162 2 22 -2.017635493208985 2 23 -1.4880974301938394 2 24 -2.2409990715202994 2 25 -2.0769847330206233 2 26 -2.09726508281161 2 27 -1.4859002830849732 2 28 -2.38195149848098 2 29 -1.9463640774039348 2 30 -1.5052670555521672 2 31 -0.7188126182070533 2 32 -2.249090844391148 2 33 -2.8875535614816243 2 34 -1.9365633433496399 2 35 -3.09349186764765 2 36 -2.380218090804142 2 37 -1.5015131517523557 2 38 -2.5363591829037873 2 39 -6.382978937003855 2 40 -2.0633925335599628 2 41 -2.092922615523257 2 42 -1.3903634492418888 2 43 -1.8315392109604574 2 44 -2.1282750288111387 2 45 -2.157296101999897 2 46 -2.0429609548981276 2 47 -3.4224144750389947 2 48 -2.893913763192476 2 49 -1.7831414619079133 2 50 -1.9577386443673284 2 51 -3.0014811558073866 2 52 -3.2011219431197944 2 53 -3.6939832178439893 2 54 -3.870065047161127 2 55 -4.057984650284727 2 56 -3.4973811783986086 2 57 -3.433852285821981 2 58 -3.128441210845466 2 59 -3.367206985411581 2 60 -2.942282141275289 2 61 -2.0985925128127185 2 62 -2.108079869069787 2 63 -1.7877927535889029 2 64 -2.16835101981005 3 1 -0.5824493971623852 3 2 -0.5989156686912835 3 3 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-0.2934918676476457 119 36 1.1340676234815774 119 37 0.1556297053905027 119 38 0.6922122456676405 119 39 1.3884496344247168 119 40 1.4508931807257497 119 41 0.935648813048175 119 42 0.03820797932953468 119 43 0.6541750747538302 119 44 0.15743925690314597 119 45 1.1569896122858125 119 46 -1.2429609548981304 119 47 -0.5081287607532801 119 48 -0.6939137631924766 119 49 -0.9831414619079126 119 50 0.27083278420410295 119 51 1.3985188441926155 119 52 1.9703066283087765 119 53 0.1060167821560114 119 54 0.9870778099817272 119 55 0.5134439211438462 119 56 1.1597616787442497 119 57 0.937576285606589 119 58 0.7858445034402486 119 59 -0.11006412826872491 119 60 0.20057500158185348 119 61 1.0156932014729954 119 62 0.6062058452159285 119 63 1.383635817839668 119 64 0.83164898018995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 62.0 5 8.0 6 16.0 7 31.0 8 57.0 9 44.0 10 63.0 11 67.0 12 53.0 13 88.0 14 90.0 15 106.0 16 154.0 17 170.0 18 139.0 19 190.0 20 212.0 21 231.0 22 290.0 23 289.0 24 290.0 25 364.0 26 446.0 27 494.0 28 583.0 29 721.0 30 899.0 31 1092.0 32 1421.0 33 1787.0 34 2126.0 35 2270.0 36 2920.0 37 3937.0 38 5903.0 39 6853.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 98.79462216040797 0.9388038942976357 0.14777468706536856 0.11879925822902179 8 98.41113366193099 1.032183241519281 0.35952449985503043 0.1971585966946941 9 96.43022716736208 2.3093416782568386 0.6055864626796477 0.6548446917014372 10 40.68150208623088 37.491307371349095 7.858136300417246 13.969054242002782 11 21.980760315252663 33.48400556328234 25.75046360686138 18.784770514603615 12 48.1368799258229 18.30667593880389 21.40125173852573 12.155192396847474 13 23.200625869262865 22.687760778859527 31.28766805748725 22.823945294390356 14 31.00081131200742 27.09202596198424 25.14487714418173 16.76228558182661 15 36.44114491198562 22.6285416002088 24.635327552707132 16.294985935098456 16 18.447671216995623 24.5485899776831 25.278961249746402 31.724777555574878 17 24.213334880078886 23.1489805980105 17.487891882486007 35.14979263942461 18 24.681270282800185 36.71186833565137 18.834028743625407 19.77283263792304 19 22.554473806212332 26.744320815948075 31.079044969865556 19.622160407974036 20 22.37356904796406 30.99550789740617 17.66990291262136 28.961020142008405 21 24.524802967083914 30.119958275382473 24.637807139545664 20.71743161798795 22 22.566063977746868 33.382591562355124 20.41029207232267 23.641052387575336 23 29.554937413073713 31.310848400556328 21.45050996754752 17.683704218822438 24 20.353520718632282 40.53028107794842 22.48623587365981 16.62996232975949 25 20.822823345220492 25.82992664753124 29.427966715954884 23.919283291293382 26 23.45814977973568 25.431833990262 23.663923023417574 27.44609320658474 27 18.548738348964836 24.103487325415955 25.755683962948982 31.592090362670227 28 17.864917423736685 29.5126694337213 27.483818535396047 25.138594607145965 29 21.31642336825609 26.60997765330702 28.70534289113968 23.368256087297212 30 15.733697230126008 36.891005168108705 32.75942163637419 14.615875965391092 31 19.335347432024168 32.98861259586335 34.67057866604694 13.005461306065536 32 12.684682592667848 32.06873530521611 42.64317436358886 12.603407738527183 33 13.81854089219331 33.92483736059479 37.35188197026022 14.904739776951672 34 14.178084184192919 34.09837973063385 38.29304476830439 13.430491316868837 35 13.074800290486566 37.15032679738562 39.02106027596224 10.753812636165577 36 12.476744186046512 33.625 39.75581395348837 14.142441860465116 37 12.346217607538609 32.143791990227726 43.082337201524005 12.427653200709653 38 10.779923229033383 32.96207979527742 44.39630103524485 11.861695940444342 39 11.033637716252882 33.36931470081979 42.87423053359395 12.72281704933337 40 11.526380082601362 33.724041649700425 43.90378686522018 10.84579140247804 41 9.881250363816289 35.150474416438676 43.36690144944409 11.601373770300949 42 10.816558394266904 34.48888629941445 43.40897835523057 11.285576951088066 43 12.836046951912156 33.317799201934 42.62662744298488 11.219526403168963 44 10.542405450257665 33.65650566279442 44.91513087023612 10.885958016711793 45 11.184248849536903 36.71870449117493 41.79239238073047 10.304654278557699 46 10.939229738390724 36.23783720794733 42.644059896288525 10.178873157373419 47 11.16322428409124 36.967984385469165 42.403938590613805 9.464852739825794 48 12.432274978153218 37.28226041363239 41.334110107777455 8.951354500436935 49 10.249985432084378 37.61144455451314 42.27609113688013 9.862478876522347 50 9.960031508008285 38.62064941505966 41.35717828281355 10.062140794118505 51 9.361702127659575 39.475371611774996 42.238414456426696 8.924511804138735 52 11.018159559273617 38.14381904567581 40.85463608010027 9.983385314950302 53 10.675025513923313 39.367254701851586 40.30325120279924 9.654468581425864 54 10.81955742149917 38.98947491180501 40.72130382809995 9.469663838595878 55 11.670020120724347 39.22083223981571 39.31122969702272 9.797917942437232 56 12.207572487019428 39.72055305991483 38.612099644128115 9.459774808937635 57 11.56740360496996 40.23508137432188 39.24050632911392 8.957008691594236 58 12.369027814260281 39.36316142777924 37.466071272217846 10.801739485742639 59 11.858272772378367 39.255173218924206 38.50801155765694 10.378542451040481 60 12.691274383833665 38.69290970680995 37.0926293657283 11.52318654362808 61 12.834052981678143 38.94270043178901 36.6728906523515 11.55035593418135 62 11.749088256746901 39.328956965718454 35.7490882567469 13.172866520787746 63 12.75199113107915 40.584065116550455 34.7025702366018 11.961373515768592 64 14.719626168224298 40.11682242990654 33.93983644859813 11.22371495327103 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 33.5 3 67.0 4 42.5 5 22.0 6 26.0 7 80.5 8 135.0 9 149.5 10 163.5 11 163.0 12 173.0 13 270.0 14 357.0 15 431.0 16 537.5 17 570.0 18 748.0 19 926.0 20 1027.0 21 1286.0 22 1444.0 23 1588.0 24 1952.5 25 2173.0 26 2461.5 27 2195.5 28 1641.0 29 1697.5 30 1500.5 31 1247.0 32 1193.0 33 1139.0 34 1123.0 35 1151.5 36 1196.0 37 1145.0 38 1013.5 39 933.0 40 836.0 41 759.0 42 779.0 43 864.5 44 950.0 45 914.0 46 863.0 47 848.0 48 797.5 49 816.0 50 885.0 51 910.0 52 1001.0 53 1067.0 54 1088.5 55 932.0 56 754.0 57 689.5 58 625.0 59 559.0 60 445.0 61 397.0 62 317.5 63 220.0 64 202.0 65 166.5 66 104.5 67 78.0 68 73.0 69 68.0 70 59.0 71 46.5 72 43.0 73 33.5 74 15.0 75 6.0 76 6.5 77 5.0 78 3.0 79 1.5 80 1.0 81 2.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.06374594343996291 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08402874362540565 16 0.0260778859527121 17 0.08982382939267501 18 0.0 19 0.0 20 0.020282800185442745 21 0.0 22 0.0 23 0.0 24 0.005795085767269355 25 0.06084840055632823 26 0.02318034306907742 27 0.2115206305053315 28 0.170955030134446 29 0.1593648585999073 30 0.20282800185442745 31 0.25498377375985165 32 0.17675011590171535 33 0.23180343069077425 34 0.3911682892906815 35 0.25208623087621695 36 0.3245248029670839 37 0.3737830319888734 38 0.35929531757070005 39 0.6809225776541493 40 0.3766805748725081 41 0.4462216040797403 42 0.5360454334724154 43 0.5186601761706073 44 0.4780945757997218 45 0.5157626332869727 46 0.53894297635605 47 0.5360454334724154 48 0.5273528048215114 49 0.5505331478905887 50 0.6809225776541493 51 0.585303662494205 52 0.5939962911451089 53 0.6287668057487251 54 0.6171766342141863 55 0.6345618915159944 56 0.6664348632359759 57 0.6548446917014372 58 0.7214881780250348 59 0.7214881780250348 60 0.7765414928140937 61 0.683820120537784 62 0.6867176634214186 63 0.6809225776541493 64 0.7881316643486325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 34512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.29937413073714 #Duplication Level Percentage of deduplicated Percentage of total 1 83.45725744671263 53.66249420491423 2 8.467396692352755 10.88896615669912 3 3.312153575773962 6.389082058414465 4 1.73043125591456 4.450625869262865 5 0.9418232616826642 3.0279323133982383 6 0.5047091163084133 1.9471488178025034 7 0.30643053490153666 1.3792304126101067 8 0.23883556396737415 1.2285581826611034 9 0.18475958722004418 1.0691933240611962 >10 0.7750890000450633 7.800185442744553 >50 0.05858230814294083 2.7932313398238295 >100 0.0135189941868325 0.9388038942976357 >500 0.009012662791221666 4.424547983310153 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 944 2.735280482151136 No Hit ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 1.6892675011590172 No Hit ATTCCAGGGGCTATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAA 112 0.3245248029670839 No Hit ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC 107 0.3100370885489105 No Hit ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG 105 0.30424200278164115 No Hit ATTCCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.2897542883634678 No Hit ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.2665739452943904 No Hit ATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.260778859527121 No Hit ATTCCAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.25498377375985165 No Hit ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.24049605934167825 No Hit ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 77 0.22311080203987016 No Hit ATTCCAGGGGAAACCCTGCTTGCTGGCTAGCTTACTGGCTCACCATGGCCAGCTAGTTTTCTTT 73 0.2115206305053315 No Hit ATTCCAGGGCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT 68 0.19703291608715812 No Hit ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.19703291608715812 No Hit ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.17675011590171535 No Hit ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACG 57 0.16515994436717663 No Hit ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.16226240148354196 No Hit ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG 51 0.14777468706536856 No Hit ATTCCAGGGATCTTCACACACTGTAAGCCAAAGCAAATATCCCCCTTCTTACGTTGTCTTTGTC 48 0.13908205841446453 No Hit ATTCCAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.1332869726471952 No Hit ATTCCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.12749188687992583 No Hit ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG 44 0.12749188687992583 No Hit ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.11879925822902179 No Hit ATTCCAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37 0.10720908669448308 No Hit ATTCCAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.1043115438108484 No Hit ATTCCAGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.10141400092721373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.011590171534538712 0.0 0.0 0.0 0.0 7 0.014487714418173389 0.0 0.0 0.0 0.0 8 0.014487714418173389 0.0 0.0 0.0 0.0 9 0.014487714418173389 0.0 0.0 0.0 0.0 10 0.0260778859527121 0.0 0.0 0.0 0.0 11 0.0521557719054242 0.0 0.0 0.0 0.0 12 0.0521557719054242 0.0 0.0 0.0 0.0 13 0.05505331478905888 0.0 0.0 0.0 0.0 14 0.0666434863235976 0.0 0.0 0.0 0.0 15 0.06954102920723226 0.0 0.0 0.0 0.0 16 0.08402874362540566 0.0 0.0 0.0 0.0 17 0.08692628650904033 0.0 0.0 0.0 0.0 18 0.09561891515994436 0.0 0.0 0.0 0.0 19 0.11010662957811776 0.0 0.0 0.0 0.0 20 0.12459434399629114 0.0 0.0 0.0 0.0 21 0.1303894297635605 0.0 0.0 0.0 0.0 22 0.1825452016689847 0.0 0.0 0.0 0.0 23 0.2086230876216968 0.0 0.0 0.0 0.0 24 0.22021325915623552 0.0 0.0 0.0 0.0 25 0.22890588780713955 0.0 0.0 0.0 0.0 26 0.24339360222531292 0.0 0.0 0.0 0.0 27 0.25208623087621695 0.0 0.0 0.0 0.0 28 0.3013444598980065 0.0 0.0 0.0 0.0 29 0.3013444598980065 0.0 0.0 0.0 0.0 30 0.33321743161798795 0.0 0.0 0.0 0.0 31 0.341910060268892 0.0 0.0 0.0 0.0 32 0.35929531757070005 0.0 0.0 0.0 0.0 33 0.3737830319888734 0.0 0.0 0.0 0.0 34 0.38537320352341214 0.0 0.0 0.0 0.0 35 0.43463143254520165 0.0 0.0 0.0 0.0 36 0.4578117756142791 0.0 0.0 0.0 0.0 37 0.46360686138154844 0.0 0.0 0.0 0.0 38 0.48678720445062584 0.0 0.0 0.0 0.0 39 0.5041724617524339 0.0 0.0 0.0 0.0 40 0.5186601761706073 0.0 0.0 0.0 0.0 41 0.5534306907742235 0.0 0.0 0.0 0.0 42 0.5824061196105702 0.0 0.0 0.0 0.0 43 0.6200741770978211 0.0 0.0 0.0 0.0 44 0.6461520630505332 0.0 0.0 0.0 0.0 45 0.6693324061196105 0.0 0.0 0.0 0.0 46 0.7041029207232267 0.0 0.0 0.0 0.0 47 0.7243857209086695 0.0 0.0 0.0 0.0 48 0.7475660639777468 0.0 0.0 0.0 0.0 49 0.791029207232267 0.0 0.0 0.0 0.0 50 0.8113120074177098 0.0 0.0 0.0 0.0 51 0.8344923504867872 0.0 0.0 0.0 0.0 52 0.846082522021326 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGTTG 15 0.0045030904 58.46696 34 GCATCGG 15 0.0045030904 58.46696 33 CATCGGA 15 0.0045030904 58.46696 34 GTTGATG 15 0.0045557935 58.295757 37 TGGTTGA 15 0.0045557935 58.295757 35 TTGATGC 15 0.0045557935 58.295757 38 ATCCTGG 35 1.5344995E-8 58.21053 53 TCCTGGC 35 1.5344995E-8 58.21053 54 GTGCATC 15 0.0045823166 58.210526 31 TTACTGG 15 0.0045823166 58.210526 32 ACTGACG 15 0.0045823166 58.210526 58 AACTGAC 15 0.0045823166 58.210526 57 TAACTGG 15 0.0045823166 58.210526 31 CTTACTG 15 0.0045823166 58.210526 31 TCATGGC 20 1.947598E-4 58.210526 54 ATCATGG 20 1.947598E-4 58.210526 53 CATGGCT 20 1.947598E-4 58.210526 55 ATCGAAC 15 0.0045823166 58.210526 53 TGATCCT 35 1.5525075E-8 58.12555 51 GATCCTG 35 1.5525075E-8 58.12555 52 >>END_MODULE