FastQCFastQC Report
Fri 17 Jun 2016
SRR1526407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30371
Sequences flagged as poor quality0
Sequence length56
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA13534.454907642158639No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7972.6242138882486583No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.5004774291264693No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.4379177504856607No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA1190.39182114517138056No Hit
ATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.3786506865101577No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1100.3621876131836291No Hit
ATTCCAGGGGCTATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA1070.35230976918771195No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.3391393105264891No Hit
ATTCCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.33255408119587765No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA840.2765796318856804No Hit
ATTCCAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA820.26999440255506896No Hit
ATTCCAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.24694609989792896No Hit
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.23048302657140035No Hit
ATTCCAGGGCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT670.22060518257548317No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG660.21731256791017745No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.20743472391426032No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.19097165058773172No Hit
ATTCCAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA520.17121596259589741No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA490.16133811859998023No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC450.14816765993875738No Hit
ATTCCAGGGGAAACCCTGCTTGCTGGCTAGCTTACTGGCTCACCATGGCCAGCTAG440.14487504527345166No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.14487504527345166No Hit
ATTCCAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.1382898159428402No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.13170458661222878No Hit
ATTCCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12841197194692305No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA380.12511935728161733No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA370.12182674261631161No Hit
ATTCCAGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT350.11524151328570018No Hit
ATTCCAGGGGTGCAGTGCTATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.11194889862039445No Hit
ATTCCAGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.10865628395508872No Hit
ATTCCAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.10865628395508872No Hit
ATTCCAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.10536366928978302No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAG320.10536366928978302No Hit
ATTCCAGGGATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAA310.1020710546244773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAA150.00797492250.58695634
CCGGGCA150.00802789950.50250233
ATAGGAT150.0083512850.0016545
AACCGCG150.00840610549.9191427
AGGGACT451.3096724E-1049.8369066
AAGTATC150.00846119749.83690310
AGGGGGC355.196125E-849.8369036
AGGGGCC204.186281E-449.8369036
AGGGGAT204.186281E-449.8369036
AGGGGAC204.186281E-449.8369036
AAACCGC150.00846119749.83690326
GGATGAG150.00846119749.8369038
GTGTTCT150.00846119749.83690313
GGGTTCC150.00846119749.8369037
GGCATGC204.186281E-449.8369038
GAAGTAT150.00846119749.8369039
TGTTCTG150.00846119749.83690314
AGGGTTT150.00846119749.8369036
AGGGTTA150.00846119749.8369036
AGGGCGG150.00846119749.8369036
Produced by FastQC (version 0.11.5)