FastQCFastQC Report
Fri 17 Jun 2016
SRR1526406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173651
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC9780.5631985994897812No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC8260.4756667108165228No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6280.36164490846583086No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5980.34436887780663517No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5670.32651697945879954No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC5330.30693747804504434No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA4870.28044756436761087No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC4800.2764164905471319No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4650.267778475217534No Hit
CTGAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4220.2430161646060201No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4070.23437814927642223No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC3030.174487909657877No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC2810.16181882050780014No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA2670.1537566728668421No Hit
CTGAAAGGGGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACT2390.1376323775849261No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2370.13648064220764639No Hit
CTGAAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG2310.13302543607580722No Hit
CTGAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2290.13187370069852752No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2260.13014609763260793No Hit
CTGAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2230.12841849456668836No Hit
CTGAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.12726675918940863No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2170.1249632884348492No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2100.1209322146143702No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG2100.1209322146143702No Hit
CTGAAAGGGCAAACTTCTTGATCTTGGGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTAT2100.1209322146143702No Hit
CTGAAAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG2090.12035634692573036No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2050.1180528761711709No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCG2020.11632527310525133No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT1970.11344593466205205No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA1950.11229419928477233No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1890.10883899315293319No Hit
CTGAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC1880.10826312546429331No Hit
CTGAAAGGGATCTTCTTCTCTTCTATCATCCAAATCAAATGTAAGAAGTCCCTCACATCCGATT1880.10826312546429331No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1860.10711139008701362No Hit
CTGAAAGGGGGGAAATCGAGTGGAAACTCTTCCTCCAGTCTGGGAGAAATGGTATCAGGAACAT1850.10653552239837374No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1840.10595965470973388No Hit
CTGAAAGGGGAAGAAGAAGGCCCCCAACAAGGCTCTGGCCTCCATCTTGGGGAAAAGCAACCTG1810.10423205164381431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG150.00462038858.1896957
CATATAC150.00462038858.1896958
TACATTC150.00462038858.1896958
ATGCGGG150.00462038858.1896957
AGATATA201.9762877E-458.17284848
TATATTA201.9762877E-458.17284851
AACGGTC201.9762877E-458.17284855
TCGTCCA201.9762877E-458.17284851
TGCTCGT201.9762877E-458.17284848
CTCACGC150.004625689758.1728456
TTCTTAC150.004625689758.1728450
TACGAAG150.004625689758.1728454
ATAAGTG150.004625689758.1728452
TTACGAA150.004625689758.1728453
ATAGTCT150.004625689758.1728448
ATAGATG150.00463099658.1560147
ACGTTCG150.004641622358.12236441
CTATCGC150.004641622358.12236441
GTGATAG150.004641622358.12236445
CGTTCGG150.004641622358.12236442
Produced by FastQC (version 0.11.5)