FastQCFastQC Report
Fri 17 Jun 2016
SRR1526405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167005
Sequences flagged as poor quality0
Sequence length64
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC7230.4329211700248496No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6010.3598694649860783No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5490.3287326726744708No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5290.3167569833238526No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4870.2916080356875543No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4460.2670578725187869No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4290.2568785365707614No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC4290.2568785365707614No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA3900.2335259423370558No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3450.20658064129816475No Hit
CTGAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3100.1856231849345828No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC2330.13951678093470254No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC2320.13891799646717162No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA2210.13233136732433162No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1990.11915810903865155No Hit
CTGAAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG1970.11796054010358972No Hit
CTGAAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCG1950.1167629711685279No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC1910.11436783329840425No Hit
CTGAAAGGGGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACT1900.11376904883087333No Hit
CTGAAAGGGCAAACTTCTTGATCTTGGGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTAT1870.11197269542828059No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT1860.11137391096074968No Hit
CTGAAAGGGGGGAAATCGAGTGGAAACTCTTCCTCCAGTCTGGGAGAAATGGTATCAGGAACAT1810.10837998862309511No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1800.1077812041555642No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1790.10718241968803328No Hit
CTGAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1790.10718241968803328No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1770.10598485075297145No Hit
CTGAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.10299092841531692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG150.004579676358.31881337
AATCGTT150.004585165558.30117839
TAATCGT150.004585165558.30117838
GATTTAT150.004596158458.26593835
CGTAAAT258.436131E-658.23074733
GACGCGT150.00461820358.19559558
CGGCAAC150.004623726558.17803255
TTAGGAT150.00462925558.1604848
CGTGCCA150.00462925558.1604847
CGAATGA201.9781312E-458.1604848
CGTCCAA150.00462925558.1604852
ATAATGC150.004634788358.1429445
ATACTCG150.004634788358.1429444
TAGATTG150.004634788358.1429445
ATTATCT150.004634788358.1429446
TCTTGCC258.557745E-658.0903942
TACTACT150.004651417558.0903942
ACCATTC150.004656970558.0728943
TTTATAC150.004656970558.0728941
TCTAGTC150.004684809657.98556528
Produced by FastQC (version 0.11.5)