SRR1526404_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526404_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156091
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1622	1.0391374262449469	No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	916	0.5868371654996124	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	736	0.4715198185673741	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	595	0.38118789680378756	No Hit
CTGAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	532	0.3408268253775042	No Hit
CTGAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	420	0.2690738095085559	No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	397	0.25433881517832546	No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	396	0.25369816325092415	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	367	0.23511925735628575	No Hit
CTGAAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	286	0.18322645123677855	No Hit
CTGAAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	275	0.17617928003536398	No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT	252	0.16144428570513356	No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC	248	0.15888167799552824	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	229	0.1467092913749031	No Hit
CTGAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	229	0.1467092913749031	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	227	0.14542798752010044	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	219	0.14030277210088987	No Hit
CTGAAAGGGGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGA	205	0.13133364511727133	No Hit
CTGAAAGGGATCTTCTTCTCTTCTATCATCCAAATCAAATGTAAGAAGTCCCTCAC	202	0.12941168933506736	No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	201	0.12877103740766604	No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	200	0.12813038548026473	No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	193	0.12364582198845546	No Hit
CTGAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	190	0.12172386620625149	No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT	185	0.11852060656924487	No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG	184	0.11787995464184353	No Hit
CTGAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCG	181	0.11595799885963957	No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	171	0.10955147958562633	No Hit
CTGAAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	166	0.10634821994861972	No Hit
CTGAAAGGGCAAACTTCTTGATCTTGGGAGCCAGTAGACTGGTTCTTCACTTTACC	166	0.10634821994861972	No Hit
CTGAAAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT	164	0.10506691609381708	No Hit
CTGAAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGC	164	0.10506691609381708	No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	159	0.10186365645681046	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACTG	25	1.9100724E-5	50.72115	37
AAACATC	15	0.007953942	50.72115	37
CAAGACG	15	0.007953942	50.72115	37
AGGCTAT	15	0.007953942	50.72115	37
GATTTAT	20	3.9066683E-4	50.67146	35
ATATGAC	15	0.008005481	50.638382	34
ATTTCCC	20	3.9700698E-4	50.506516	33
CATTAAT	15	0.008088459	50.506508	33
ACATGAT	20	3.9956582E-4	50.440834	31
CTAGTGT	15	0.008130188	50.44083	31
CGGAGGT	15	0.008130188	50.44083	31
CATTATT	15	0.00823521	50.277378	40
AAACGCA	15	0.008245767	50.261093	48
TAACCTT	20	4.0667027E-4	50.261093	38
ATCCCTA	15	0.008245767	50.261093	48
CTCAAGT	15	0.008256334	50.24482	50
ACGCACC	15	0.008256334	50.24482	50
TTTTACG	20	4.073211E-4	50.24482	50
AACGCAC	15	0.008266912	50.22855	49
CCACACT	20	4.0862517E-4	50.2123	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content