FastQCFastQC Report
Fri 17 Jun 2016
SRR1526403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313576
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC28980.924177870755415No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC28730.9162053218358548No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC24730.7886445391228921No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC18550.5915631298313646No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT13280.42350179860703624No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC12050.3842768579228002No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10490.3345281526647447No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT9590.3058269765543281No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC8260.263413016302268No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC7690.24523560476567086No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC7070.22546368344516163No Hit
GTATACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG6870.2190856443095135No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.21111309538995332No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA6100.19453019363726817No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT4850.1546674490394673No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT4690.1495650177309488No Hit
GTATACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG4570.14573819424955992No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCCG4540.1447814883792127No Hit
GTATACGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG4510.14382478250886546No Hit
GTATACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTCTGGCTGCTCTTCCAGAGGT3730.11895042987983774No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC3680.1173559200959257No Hit
GTATACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3660.1167181161823609No Hit
GTATACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3620.11544250835523126No Hit
GTATACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.11448580248488406No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC3580.11416690052810163No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAGAGGTT3570.11384799857131922No Hit
GTATACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.11289129270097202No Hit
GTATACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTCTGGCTGCTCTTCCAGAGG3480.11097788096027757No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCCA3460.11034007704671277No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA3370.10746995943567109No Hit
GTATACGGGGGTGTTCACCCTGTTTCTCATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTG3280.10459984182462943No Hit
GTATACGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG3230.1030053320407174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA201.9775917E-458.1798558
AACGCCT406.9667294E-1058.1798558
TATATTG150.004638740358.1425446
TCCGTTA150.004638740358.1425448
CCGTTAG150.004638740358.1425449
ACACGAT150.00464168658.13321744
CTACGTA150.00464168658.13321739
TATTATC150.00464168658.13321744
TACGTAC150.00464168658.13321740
TATGGGT150.00467714458.021629
GCGATTA150.00467714458.021629
ACTTATG150.0046860457.99376327
CATAAGA150.004706845657.92891322
AATCGTA150.00473668857.8365219
GTTGCCC150.00473668857.8365219
TACGGAC351.6751073E-857.82734
CGACACA202.0379231E-457.827316
CACGTAT150.0047396857.827313
TGTACGA150.0047396857.827311
TATCGGC202.0379231E-457.827318
Produced by FastQC (version 0.11.5)