FastQCFastQC Report
Fri 17 Jun 2016
SRR1526402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302139
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC23410.7748089455515508No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC22680.7506478806112419No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC20210.6688974280049911No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC15910.5265788263018015No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT14530.4809044843598476No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC9380.3104531358083531No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8680.2872849913450432No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT7970.26378587338940024No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC6690.2214212663707764No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC5830.19295754603013845No Hit
GTATACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG5620.18600710269114545No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC5410.17905665935215248No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.17309913649015848No Hit
GTATACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG4920.16283895822783553No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA4900.16217701124316952No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT3680.12179824517854365No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT3630.12014337771687866No Hit
GTATACGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG3560.11782656327054768No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAGAGGTT3350.11087611993155469No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCCG3310.1095522259622227No Hit
GTATACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3240.1072354115158917No Hit
GTATACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTCTGGCTGCTCTTCCAGAGGT3200.1059115175465597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAT150.00459989358.2656136
ACCCGTT201.9631216E-458.2656136
TACCCGT201.9663648E-458.24616235
ATGACGC201.9712375E-458.21701439
AACGTTG150.004645675458.12006850
CTTATCC150.004645675458.12006828
TATTAGT150.004645675458.12006828
CTCGAAT150.004645675458.12006855
TTACATG150.004645675458.12006850
ATACAAC150.004645675458.12006855
AGTCTAC201.987549E-458.12006428
TTATCTC201.9908242E-458.1007247
GAGGTAA150.004651805458.1007248
CTATTAG150.00465487258.09104527
CATAGAC150.00465794158.08138331
CGAATAC150.00465794158.08138352
ATTAAGC1150.058.07171251
AACGATA150.00466408358.06205444
CTAGTCT201.999031E-458.052426
GCTAGTC201.999031E-458.052425
Produced by FastQC (version 0.11.5)