FastQCFastQC Report
Fri 17 Jun 2016
SRR1526401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276414
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC59672.158718444073021No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC29131.0538540016062863No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC18100.6548148791305796No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT12780.46234995333087325No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC11760.42544878334671904No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC11170.40410398894412003No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG11000.39795379394676106No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC10270.3715441330757487No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9400.34006960573632306No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8990.3252367825073983No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7250.26228772782854703No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA6700.24239003813120896No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC4850.17546144551289009No Hit
GTATACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4530.1638846078708025No Hit
GTATACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA4500.16279927934185678No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC4440.16062862228396535No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT4110.14869000846556252No Hit
GTATACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.14796645611293205No Hit
GTATACGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC3730.1349425137655835No Hit
GTATACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTCTGGCTGCTCTT3600.13023942347348544No Hit
GTATACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3490.12625988553401782No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG3410.12336567612349593No Hit
GTATACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA3370.12191857141823495No Hit
GTATACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.11793903347876734No Hit
GTATACGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3180.11504482406824545No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3160.11432127171561499No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG3150.11395949553929974No Hit
GTATACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCA3140.1135977193629845No Hit
GTATACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3120.11287416701035403No Hit
GTATACGGGGGTGTTCACCCTGTTTCTCATAGCCTCGTTGTGTGGCTTTGGCCCTA3020.10925640524720166No Hit
GTATACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG3000.10853285289457118No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC2890.10455331495510357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGA150.00805713950.56664736
CATCGAT150.00806299750.5573534
TCATCGA150.00812175750.4645933
AACGTGC301.0105268E-650.2433348
GTCGGAC252.0330683E-550.20664646
TGAACGT301.0156327E-650.20664246
CCTCGCA150.00829994850.1883245
CACCTAT150.00830593750.1791644
GTAGCTT355.1306415E-850.17001349
CAAGTAA150.00831792550.1608640
AGTAATA150.00831792550.1608642
CGTTGTA150.00833593250.1334339
CCGTGTC150.00833593250.1334339
ATCCGAA150.00833593250.1334339
GTACGTA301.02591E-650.1334339
AGTACGT301.0271997E-650.12429438
AATACTC150.00837203250.0786641
TCCGACT252.0930866E-549.960430
TATGAAC150.00845064849.960430
ATTAGTT150.00846279149.94225329
Produced by FastQC (version 0.11.5)