FastQCFastQC Report
Fri 17 Jun 2016
SRR1526400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850996
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC94361.1088183728243142No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC94251.1075257698038532No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC73140.8594634992408895No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC44640.5245618075760637No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT35130.41281040098895877No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC24430.28707537990777865No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC24010.2821399865569286No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT22070.25934316965062115No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC19370.22761564096658504No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC18290.21492462949297056No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT17320.20352622103981688No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAGTGGCTAAGAGCACTGACTGCTCTTCCAGAGGTCC16320.19177528449017386No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAGAGGTCC14420.16944850504585215No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT13010.1528796845108555No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT12890.15146957212489837No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC12520.14712172560153045No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC12210.1434789352711411No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA11880.13960112620975892No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.1379559950928089No Hit
GAATTAGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG11540.13560580778288028No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAAAGGTCC10290.12091713709582654No Hit
GAATTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG9900.11633427184146576No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9860.11586423437948004No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC9720.11421910326253003No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC9620.11304400960756573No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGT9280.1090486911806871No Hit
GAATTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9110.1070510319672478No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTCTGGCTGCTCTTCCAGAGGTC8840.10387827909884419No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCAAAGGTCC8760.10293820417487275No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAATATAAG8680.10199812925090129No Hit
GAATTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG8510.10000047003746199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC150.00470299857.94950525
TAGGGAT45600.056.4585575
TAGGGGC96300.056.081895
TAGGGGG325650.055.8111045
TTAGGGG584350.055.7453354
AATTAGG878150.055.6438262
ATTAGGG871600.055.6404953
GAATTAG881550.055.6195341
TAGGGGT62100.055.0592775
AGGGGGG242100.054.938756
TTAGGGT33550.054.9226534
TTAGGGC62750.054.811634
TTAGGGA194600.054.6730774
TAGGGAC31050.054.5934645
TAGGGCA30100.054.490555
TAGGGAG69700.054.326475
TAGGGTG14400.054.238285
TAGGGGA102900.054.2277345
ATACATC650.053.4729723
AGGGGAT14350.053.419356
Produced by FastQC (version 0.11.5)