FastQCFastQC Report
Fri 17 Jun 2016
SRR1526399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820712
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC76620.9335796235463841No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC75890.9246849077386465No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC59710.7275390149041321No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT37610.4582606322315258No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC34060.4150055074130755No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC19860.24198500813927418No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC19780.24101024476308378No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT18230.2221242043493942No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC14890.18142783339344376No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC14510.1767977073565392No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT14260.1737515718059441No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAGTGGCTAAGAGCACTGACTGCTCTTCCAGAGGTCC12950.15778982152082582No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAGAGGTCC11710.14268098918987415No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT11080.1350047276023745No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT10980.13378627338213647No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC10690.13025275614344617No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.12062696780356567No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC9720.11843375020713723No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA9650.11758083225297059No Hit
GAATTAGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG9600.11697160514285157No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAAAGGTCC8460.10308122703213796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTA150.0047586157.77749619
TAGGGGC92150.055.837225
TAGGGAT39300.055.7961735
TAGGGGG311450.055.7866485
TTAGGGG557700.055.745164
AATTAGG844650.055.5851142
ATTAGGG838550.055.5691383
GAATTAG850050.055.527841
TAGGGAG65950.055.371655
TAGGGAC31050.055.26885
AGGGGGG229100.054.9941066
TTAGGGA184350.054.8347634
TTAGGGC63850.054.7041854
TAGGGGA95650.054.669965
TAGGGGT61400.054.3932045
TTAGGGT35350.053.9398424
TAGGGCA30950.053.767155
TAGGGCC9300.053.7425585
AGGGATA4100.053.5531356
TAGGGTC9200.053.3846365
Produced by FastQC (version 0.11.5)