FastQCFastQC Report
Fri 17 Jun 2016
SRR1526398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744958
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC198132.659613025163835No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC103531.3897427774451714No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC43320.5815092931413584No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT32430.43532655532258197No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC24130.32391087819716013No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC22150.29733219859374627No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC20550.27585447770209864No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT19680.2641759669672653No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT14640.19652114615857535No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTC14510.19477608133612903No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAGTGGCTAAGAGCACTGACTGCTCTTCC14450.19397066680269223No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC14410.19343372378040102No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC13560.18202368455671325No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC13200.17719119735609257No Hit
GAATTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG12800.17182176713318068No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12690.17034517382187989No Hit
GAATTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12290.164975743598968No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA11920.1600090206427745No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC11620.15598194797559056No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC10710.14376649421846602No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC10680.14336378695174762No Hit
GAATTAGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC10420.1398736573068549No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCT10040.13477269859508859No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC9700.13020868290561347No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC9080.12188606606010004No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC8710.11691934310390653No Hit
GAATTAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8560.11490580677031458No Hit
GAATTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT7630.10242188150204441No Hit
GAATTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC7540.10121375970188924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATT150.00803857750.60476736
CGTATAG150.00814321750.4394732
GTTCGAA204.101201E-450.20033646
TAGTTCG204.106686E-450.1867444
ACGATAC150.00830651950.1867445
TCGACAC150.00831541550.1731543
AATCGGC150.00831541550.1731543
ATCGGGT150.0084835349.9197229
CGATCGA150.00858560549.76888319
TAGACGC252.1498487E-549.7488415
TAATCGT150.00859928349.7488418
TAGGGAT40600.048.5012975
TAGGGGC84900.048.3521585
TTAGGGG502100.048.2122764
TAGGGGG275750.048.0916985
TAGGGGT54150.048.0879365
ATTAGGG761800.047.9883583
AATTAGG766850.047.9807362
GAATTAG768800.047.9658971
AGGGGGG200350.047.515086
Produced by FastQC (version 0.11.5)