FastQCFastQC Report
Fri 17 Jun 2016
SRR1526397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66819
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG42116.302099702180517No Hit
AGTCGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC12451.8632424909082745No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.5058441461261018No Hit
AGTCGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.465436477648573No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2410.36067585566979454No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2280.34122031158802135No Hit
AGTCGTGGGGCTTGGAAAATTGTTTTTCAACTTTTTACTCGGAGGTAGTGTCTGCCTTCGTCAC2070.3097921249943878No Hit
AGTCGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.24843233212110327No Hit
AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1480.2214938864694174No Hit
AGTCGTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1320.19754860144569658No Hit
AGTCGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1280.19156228018976637No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.179589637677906No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG1120.16761699516604558No Hit
AGTCGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1070.16013409359613284No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1060.15863751328215028No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1040.1556443526541852No Hit
AGTCGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.15265119202622007No Hit
AGTCGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.13169906763046438No Hit
AGTCGTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.1287059070024993No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC860.1287059070024993No Hit
AGTCGTGGGTATGTGGTCAGCACAGGTAATTTGTAATGAATGAAGCAGTAGGCAGTTTCTAATG840.1257127463745342No Hit
AGTCGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA840.1257127463745342No Hit
AGTCGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGGGCAAAGAG810.12122300543258653No Hit
AGTCGTGGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAG790.11822984480462143No Hit
AGTCGTGGGGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGC780.11673326449063888No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC730.10925036292072615No Hit
AGTCGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC720.10775378260674359No Hit
AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.10775378260674359No Hit
AGTCGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGGGAGCAAAGAG700.1047606219787785No Hit
AGTCGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC700.1047606219787785No Hit
AGTCGTGGGGTTCATTTTAATTTGGTGTTCTCTCCCCTCCTCTCCCTTTACCCTCAGTACCAAG680.1017674613508134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC150.0046062258.1950346
AGCAACA150.0046062258.1950353
TCGTTGA150.0046062258.1950355
AGCCGAT150.0046062258.1950347
CGAACTG150.0046062258.1950354
GTTCTGG150.0046062258.1950348
CTTTTAT150.0046062258.1950353
TATGGTT150.0046062258.1950348
GCAACAC150.0046062258.1950354
TTGTCCC150.0046062258.1950353
ACACAAT150.0046062258.1950352
TATATTA150.0046062258.1950357
CGACATT150.0046062258.1950352
CAACACC150.0046062258.1950355
ATCAATA201.9636894E-458.1950354
TTATATA201.9636894E-458.1950351
GCCGATG150.0046062258.1950348
CACTCAG150.0046062258.1950345
AACATCG150.0046062258.1950349
AAGCAAC150.0046062258.1950352
Produced by FastQC (version 0.11.5)